Renin Antibody

Images

 
Western Blot: Renin Antibody [NBP1-31559] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renin antibody [N2C2], Internal diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Renin Antibody [NBP1-31559] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Renin protein stained by Renin antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: Renin Antibody [NBP1-31559] - Paraffin-embedded OVCA xenograft, using antibody at 1:100 dilution.
Western Blot: Renin Antibody [NBP1-31559] - A. 30 ug K562 whole cell lysate/extract 10 % SDS-PAGERenin antibody [N2C2], Internal dilution: 1:1000

Product Details

Summary
Reactivity Hu, Rt, Mk, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Renin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Renin. The exact sequence is proprietary.
Localization
Secreted, Membrane
Predicted Species
Primate (100%), Monkey (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
REN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Rhesus Monkey (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Renin Antibody

  • angiotensin-forming enzyme
  • Angiotensinogenase
  • EC 3.4.23
  • EC 3.4.23.15
  • FLJ10761
  • HNFJ2
  • REN
  • renin precursor, renal
  • Renin

Background

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Renin also occurs in other organs than the kidney, e.g., in the brain, where it is implicated in the regulation of numerous activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol REN
Entrez