Human RENBP ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. RENBP (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of human RENBP. |
| Standard Curve Range |
0.195 to 50 ug/mL |
| Sensitivity |
0.17 ug/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
CV% < 7.2% |
| Intra-Assay |
CV% < 4.5% |
| Spike Recovery |
97% |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
RENBP |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated RBP: 1 vial, lyophilized.
- Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine (8 mL).
- MIX Diluent Concentrate (10×): A 10-fold concentrated buffered protein base (30 mL).
- RBP Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human RBP.
- RBP Standard: Human RBP in a buffered protein base (100 µg, lyophilized).
- Sealing Tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes, which can be cut to fit the format of the individual assay.
- Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction (12 mL).
- Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate (80 µL).
- Wash Buffer Concentrate (20×): A 20-fold concentrated buffered surfactant (30 mL, 1 bottle).
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human RENBP ELISA Kit (Colorimetric)
Background
RENBP, also known as N-acylglucosamine 2-epimerase, is a 49kDa 427 amino acid protein with a shorter 29kDa 254 isoform produced by alternative splicing. RENBP functions as a catalyzer in the conversion of N-acetylglucosamine to N-acetylmannosamine. Current research is being conducted on RENBP in relation to sialuria, Wilms tumor, allergic rhinitis, myopathy, vascular disease, hypertension, nephropathy, proteinuria and aldosteronism. RENBP has been linked to the amino sugar and nucleotide sugar metabolism pathways where it interacts with HEXA, HEXB, GNE, REN and ZBED1.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for RENBP ELISA Kit (KA1838). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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