Reg3B Antibody (518630)

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg3B Monoclonal Antibody (Catalog # MAB5110) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Reg3B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 518630
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Reg3B Antibody (518630) Summary

• Immunogen: E. coli-derived recombinant mouse Reg3B
  
  Glu27-Gly175
  Accession # P35230
• Specificity: Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) Reg3A, rmReg3D, recombinant human Reg3D, recombinant rat (rr) Reg3B, or rrReg3G.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Reg3b
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg3B Antibody (518630)

• PAP1
• Reg3B

Background

Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted, 17 kDa member of the Reg gene family, C-type lectin gene superfamily.  It is found in pancreatic acinar cells, intestinal enterocytes and developing spinal motoneurons.  Reg3B may play a role in intestinal epithelial cell renewal. It contains one C-type lectin domain.  Mature mouse Reg3B is 91% and 67% aa identical to rat and human Reg3B, respectively.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Reg3B Antibody (MAB5110)(9)

Publications using MAB5110

• FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):. 2021-03-03 [PMID: 33676046] (Neutralization, Mouse)
• WC Liu, PH Chen, LW Chen Supplementation of endogenous Ahr ligands reverses insulin resistance and associated inflammation in an insulin-dependent diabetic mouse model J. Nutr. Biochem., 2020-04-01;83(0):108384. 2020-04-01 [PMID: 32512500] (Western Blot, Mouse)
• TB Tsay, MC Yang, WH Chang, PH Chen, LW Chen Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model J Transl Med, 2018-08-13;16(1):225. 2018-08-13 [PMID: 30103798] (Mouse)
• S Bluemel, L Wang, C Martino, S Lee, Y Wang, B Williams, A Horvath, V Stadlbauer, K Zengler, B Schnabl The Role of Intestinal C-type Regenerating Islet Derived-3 Lectins for Nonalcoholic Steatohepatitis Hepatol Commun, 2018-02-28;2(4):393-406. 2018-02-28 [PMID: 29619418] (Western Blot, Mouse)
• S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration Cell Stem Cell, 2017-12-14;22(1):35-49.e7. 2017-12-14 [PMID: 29249464] (IHC, Mouse)
• Caroline A. Lindemans, Marco Calafiore, Anna M. Mertelsmann, Margaret H. O’Connor, Jarrod A. Dudakov, Robert R. Jenq et al. Interleukin-22 promotes intestinal-stem-cell-mediated epithelial regeneration Nature 12/24/2015 [PMID: 26649819]
• Wu Y, Hsu C, Chen P, Fung C, Chen L Toll-like receptor stimulation induces nondefensin protein expression and reverses antibiotic-induced gut defense impairment. Infect Immun, 2014-03-04;82(5):1994-2005. 2014-03-04 [PMID: 24595141] (Western Blot, Mouse)
• Zechner D, Spitzner M, Bobrowski A, Knapp N, Kuhla A, Vollmar B Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice. Diabetologia, 2012-02-12;55(5):1526-34. 2012-02-12 [PMID: 22327285] (IHC, Western Blot, Mouse)
• Marleen T. J. van Ampting, Linda M. P. Loonen, Arjan J. Schonewille, Irene Konings, Carolien Vink, Juan Iovanna et al. Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice Infection and Immunity 3/1/2012 [PMID: 22252863]

Bioinformatics

• Gene Symbol: Reg3b
• Uniprot:
  • P35230()