Reelin Antibody (G10) - BSA Free

Immunohistochemistry-Frozen: Reelin Antibody (G10) [NB600-1081] - Frozen mouse brain coronal section stained with Reelin Antibody (G10). IHC-Fr image submitted by a verified customer review.

Immunohistochemistry: Reelin Antibody (G10) [NB600-1081] - Mouse anti-Reelin staining of embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone, positive for reelin using NB600-1081.

Immunohistochemistry: Reelin Antibody (G10) [NB600-1081] - Mouse anti-Reelin. Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS. The blue stain is Hoechst nuclear counter stain. Photo courtesy of Dr. Jason Emsley, Harvard Medical School, Boston, MA.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Reelin Antibody (G10) - BSA Free Summary

• Immunogen: Recombinant fusion protein, corresponding to amino acids 164-496 of Mouse Reelin (derived from E15-E17 embryonic brain).
• Localization: Secreted
• Specificity: Reelin (G10)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RELN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 27915032).
• Theoretical MW: 388 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Phosphate and 0.25M NaCl
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Reelin Antibody (G10) - BSA Free

• EC 3.4.21
• EC 3.4.21.-
• Reeler
• Reelin
• RELN
• RL
• RLPRO1598

Background

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Reelin Antibody (NB600-1081)(12)

Publications using NB600-1081

• Wang ZH, Liu Y, Chaitankar V et al. Yin Yang 1 sustains biosynthetic demands during brain development in a stage-specific manner Nat Commun 2019-05-16 [PMID: 31097699] (IHC-P, Mouse)
• Carvajal AE, Vazquez-Carretero MD, Garcia-Miranda P et al. Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis. Biochim. Biophys. Acta. 2016-11-30 [PMID: 27915032] (ICC/IF, WB, Mouse)
• Shiraki A, Tanaka T, Watanabe Y et al. Immunohistochemistry of aberrant neuronal development induced by 6-propyl-2-thiouracil in rats. Toxicol. Lett. 2016-08-20 [PMID: 27553673] (IF/IHC)
• Akane H, Shiraki A, Imatanaka N et al. Glycidol induces axonopathy and aberrations of hippocampal neurogenesis affecting late-stage differentiation by exposure to rats in a framework of 28-day toxicity study. Toxicol Lett. 2013-11-01 [PMID: 24185127] (IF/IHC, Rat)
• Falk S, Joosten E, Kaartinen V et al. Smad4 and Trim33/Tif1gamma Redundantly Regulate Neural Stem Cells in the Developing Cortex. Cereb Cortex. 2013-06-13 [PMID: 23765158] (IHC-P, Mouse)
• Akane H, Shiraki A, Imatanaka N et al. Glycidol induces axonopathy by adult stage-exposure and aberration of hippocampal neurogenesis affecting late-stage differentiation by developmental exposure in rats. Toxicol Sci 2013-04-17 [PMID: 23596259] (IHC-P, Rat)
• Wang L, Ohishi T, Akane H et al. Reversible effect of developmental exposure to chlorpyrifos on late-stage neurogenesis in the hippocampal dentate gyrus in mouse offspring. Reprod Toxicol 2013-02-18 [PMID: 23428981] (IHC-P, Mouse)
• Ohishi T, Wang L, Akane H et al. Reversible effect of maternal exposure to chlorpyrifos on the intermediate granular cell progenitors in the hippocampal dentate gyrus of rat offspring Reprod Toxicol 2012-10-22 [PMID: 23099338] (IF/IHC, Rat)
• Ohishi T, Wang L, Akane H, Shiraki A, Goto K, Ikarashi Y, Suzuki K, Mitsumori K, Shibutani M. Reversible aberration of neurogenesis affecting late-stage differentiation in the hippocampal dentate gyrus of rat offspring after maternal exposure to manganese chloride. Reproductive Toxicology, 10.1016/j.reprotox.2012.04.00. 2012-05-04 [PMID: 22561194] (IF/IHC, Rat)
• Saegusa Y, Fujimoto H, Woo GH et al. Transient aberration of neuronal development in the hippocampal dentate gyrus after developmental exposure to brominated flame retardants in rats Arch Toxicol 2012-03-14 [PMID: 22415764] (IF/IHC, Mouse)
• Ogawa B, Ohishi T, Wang L et al. Disruptive neuronal development by acrylamide in the hippocampal dentate hilus after developmental exposure in rats Arch Toxicol 2011-08-01 [PMID: 21120452] (IF/IHC, Rat)
• Saegusa Y, Woo GH, Fujimoto H et al. Sustained production of Reelin-expressing interneurons in the hippocampal dentate hilus after developmental exposure to anti-thyroid agents in rats. Reprod Toxicol 2010-07-01 [PMID: 20347957] (IF/IHC, Rat)

Review for Reelin Antibody (NB600-1081) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Bhavin Shah Immunofluorescence in frozen sections Mouse 11/25/2021

Summary

• Application: Other
• Sample Tested: 20 um fixed frozen mouse brain coronal section
• Species: Mouse
• Lot: 3494056

Comments

• Comments: Antigen Retrievel: Using 10mM sodium citrate and boiling in a microwave for 2 min. The antibody works without the antigen retrieval also.

Bioinformatics

• Gene Symbol: RELN
• Entrez:
  • 5649(Human)
  • 19699(Mouse)
  • 24718(Rat)
• Uniprot:
  • P78509()