Recombinant Rat Reg3B Protein, CF

1 μg/lane of Recombinant Rat Reg3B (Catalog # 8288-RG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 15.6 kDa and 14.5 kDa, respectively.

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat Reg3B Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED₅₀ for this effect is 0.2-1.0 μg/mL.
• Source: E. coli-derived rat Reg3B protein
  Glu27-Gly175, with an N-terminal Met
• Accession #: P25031
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Reg3b
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 16 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Reg3B Protein, CF

• PAP1
• Reg3B

Background

Rat Reg3B (Islets of Langerhans regenerating protein 3), also known as Reg-III alpha, Lithostathine 3, pancreatitis associated protein 2 and Lithostatine 3 is a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). Reg3B has been categorized in the type 3 subclass of the Reg gene family (3). The rat Reg3B cDNA encodes 174 amino acids (aa) including the 25 aa signal sequence and the 149 aa secreted mature protein. Rat Reg3B shares approximately 83% aa sequence identity with mouse Reg3B and 55% with humans Reg3B (1, 2). Reg proteins are thought to be stress response proteins that stimulate proliferation, particularly in  beta  cells of pancreatic islets (1). Reg3B is expressed primarily by acinar cells in the normal exocrine pancreas, and is up-regulated in NOD (non-obese diabetic) islets, hyperplastic islets, regenerating islets or in response to pancreatic injury or pancreatitis (1-6). IFN-beta causes Reg3B up-regulation in early-onset diabetic NOD mice or in a NOD insulinoma cell line (5). Reg3B is also up-regulated in mice lacking the pancreas-expressed keratin K8, and is thought to be regulated by keratin filament organization (4). It is proposed to serve as an autoantigen in NOD mouse immune-mediated type I diabetes models (5).

1. Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.
2. Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
3. Tebar LA. et al. (2008) PNAS. 105:11400.
4. Zhong, B. et al. (2007) Mol. Biol. Cell 18:4969.
5. Gurr, W. et al. (2007) Diabetes 56:34.
6. Lu, Y. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 291:E50.
7. Baeza, N. et al. (1997) FEBS Lett. 416:364.
8. Planas, R. et al. (2006) Diabetologia 49:2379.

Bioinformatics

• Gene Symbol: Reg3b
• Uniprot:
  • P25031()