Recombinant Rat IFN-gamma Protein


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Reactivity RtSpecies Glossary
Applications Bioactivity

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Recombinant Rat IFN-gamma Protein Summary

Details of Functionality
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.1‑0.6 ng/mL.
E. coli-derived rat IFN-gamma protein
Glu23-Cys156, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
585-IF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and PEG with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma


Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37%‑45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma . IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN‑ gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Dijkema, R. et al. (1985) EMBO J. 4:761.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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Publications for IFN-gamma (585-IF)(38)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), In Vivo, Stimulation.

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Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using 585-IF Applications Species
AA Perez-Sern, RS Dos Santos, C Ripoll, A Nadal, DL Eizirik, L Marroqui BCL-XL Overexpression Protects Pancreatic beta-Cells against Cytokine- and Palmitate-Induced Apoptosis International Journal of Molecular Sciences, 2023-03-16;24(6):. 2023-03-16 [PMID: 36982731] (Bioassay, Rat) Bioassay Rat
A Badia-Sote, J de Vries, W Dykstra, LM Broersen, JM Verkuyl, AB Smit, MHG Verheijen High-Throughput Analysis of Astrocyte Cultures Shows Prevention of Reactive Astrogliosis by the Multi-Nutrient Combination Fortasyn Connect Cells, 2022-04-22;11(9):. 2022-04-22 [PMID: 35563732] (Bioassay, Mouse) Bioassay Mouse
S Kim, Y Son Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia International Journal of Molecular Sciences, 2021-08-16;22(16):. 2021-08-16 [PMID: 34445510] (Bioassay, Rat) Bioassay Rat
N Javeed, TK Her, MR Brown, P Vanderboom, K Rakshit, AM Egan, A Vella, I Lanza, AV Matveyenko Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes Cell Reports, 2021-08-24;36(8):109613. 2021-08-24 [PMID: 34433033] (Stimulation, Mouse) Stimulation Mouse
KM Pusic, RP Kraig, AD Pusic IFNgamma-stimulated dendritic cell extracellular vesicles can be nasally administered to the brain and enter oligodendrocytes PLoS ONE, 2021-08-13;16(8):e0255778. 2021-08-13 [PMID: 34388189] (Bioassay, Rat) Bioassay Rat
Y Wang, LO Sangaré, TC Paredes-Sa, MA Hassan, S Krishnamur, AM Furuta, BM Markus, S Lourido, JPJ Saeij Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFN&amp;gamma-activated murine macrophages Nat Commun, 2020-10-16;11(1):5258. 2020-10-16 [PMID: 33067458] (Bioassay, Rat) Bioassay Rat
S Vig, M Buitinga, D Rondas, I Crèvecoeur, M van Zandvo, E Waelkens, DL Eizirik, C Gysemans, P Baatsen, C Mathieu, L Overbergh Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells Cell Death Dis, 2019-04-05;10(4):309. 2019-04-05 [PMID: 30952835] (Bioassay, Rat) Bioassay Rat
S Lively, LC Schlichter Microglia Responses to Pro-inflammatory Stimuli (LPS, IFN?+TNF?) and Reprogramming by Resolving Cytokines (IL-4, IL-10) Front Cell Neurosci, 2018-07-24;12(0):215. 2018-07-24 [PMID: 30087595] (Bioassay, Rat) Bioassay Rat
TR Nardelli, EC Vanzela, KC Benedicto, F Brozzi, A Fujita, AK Cardozo, D Eizirik, AC Boschero, F Ortis Prolactin protects against cytokine-induced beta cell death by NFkB and JNK inhibition J. Mol. Endocrinol., 2018-04-09;0(0):. 2018-04-09 [PMID: 29632026] (Bioassay, Rat) Bioassay Rat
T Erkens, N Goeminne, A Kegels, M Byloos, P Vinken Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat J Pharmacol Toxicol Methods, 2018-01-31;91(0):43-49. 2018-01-31 [PMID: 29371052] (ELISA (Standard), Rat) ELISA (Standard) Rat
Show All 38 Publications.

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  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

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Blogs on IFN-gamma.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Gene Symbol Ifng