Recombinant Rat GM-CSF Protein, CF

Images

 
There are currently no images for GM-CSF (518-GM/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Rat GM-CSF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using DA3 mouse myeloma cells. The ED50 for this effect is 5‑30 pg/mL.
Source
E. coli-derived rat GM-CSF protein
Ala1-Lys127, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met & Ala1
Protein/Peptide Type
Recombinant Proteins
Gene
Csf2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
518-GM/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat GM-CSF Protein, CF

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • molgramostin
  • sargramostim

Background

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3 - 5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices (8 - 10). Mature rat GM-CSF shares 56% ‑ 69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM‑CSF R alpha (13). Rat GM‑CSF is active on mouse cells, although mouse GM‑CSF is only weakly active on rat cells (14, 15).

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
  7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
  8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
  9. Diederichs, K. et al. (1991) Science 254:1779.
  10. Smith, L.R. et al. (1994) Immunogenetics 39:80.
  11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
  12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  14. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
  15. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.

Customers Who Viewed This Item Also Viewed...

NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
518-GM/CF
Species: Rt
Applications: Bioactivity

Publications for GM-CSF (518-GM/CF)(7)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(4)
In Vivo
(3)
All Applications
Filter By Species
Rat
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using 518-GM/CF Applications Species
JE Lim, E Chung, Y Son A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN? Sci Rep, 2017;7(1):9417. 2017 [PMID: 28842601] (Bioassay, Rat) Bioassay Rat
HT Lee, SP Liu, CH Lin, SW Lee, CY Hsu, HK Sytwu, CH Hsieh, WC Shyu A Crucial Role of CXCL14 for Promoting Regulatory T Cells Activation in Stroke Theranostics, 2017;7(4):855-875. 2017 [PMID: 28382159] (In Vivo, Rat) In Vivo Rat
Lazzaro M, Bettegazzi B, Barbariga M, Codazzi F, Zacchetti D, Alessio M Ceruloplasmin potentiates nitric oxide synthase activity and cytokine secretion in activated microglia. J Neuroinflammation, 2014;11(0):164. 2014 [PMID: 25224679] (Bioassay, Rat) Bioassay Rat
Slater, Bernard, Vilson, Fernandi, Guo, Yan, Weinreich, Daniel, Hwang, Shelly, Bernstein, Steven L Optic nerve inflammation and demyelination in a rodent model of nonarteritic anterior ischemic optic neuropathy. Invest Ophthalmol Vis Sci, 2013;54(13):7952-61. 2013 [PMID: 24065807] (In Vivo, Rat) In Vivo Rat
Quan Y, Jiang J, Dingledine R EP2 receptor signaling pathways regulate classical activation of microglia. J Biol Chem, 2013;288(13):9293-302. 2013 [PMID: 23404506] (Bioassay, Rat) Bioassay Rat
Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes. Cell. Signal., 2009;21(1):151-60. 2009 [PMID: 18950705] (Bioassay, Rat) Bioassay Rat
Reed JA, Clegg DJ, Smith KB, Tolod-Richer EG, Matter EK, Picard LS, Seeley RJ GM-CSF action in the CNS decreases food intake and body weight. J. Clin. Invest., 2005;115(11):3035-44. 2005 [PMID: 16276414] (In Vivo, Rat) In Vivo Rat

Reviews for GM-CSF (518-GM/CF) (0)

There are no reviews for GM-CSF (518-GM/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for GM-CSF (518-GM/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Albumin Antibody
NB600-41532

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Rat GM-CSF Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Csf2
Entrez
Uniprot