Recombinant Rat Fc gamma RIIA/CD32a Protein, CF

When Recombinant Rat Fc gamma RIIA/CD32a (Catalog # 9696‑CD) isimmobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated RatIgG with an ED50 of 1.25‑6.25 μg/mL.

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat Fc gamma RIIA/CD32a Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma RIIA/CD32a is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 1.25-6.25 μg/mL
• Source: Human embryonic kidney cell, HEK293-derived rat Fc gamma RIIA/CD32a protein
  Asp37-His220, with a C-terminal 6-His tag
• Accession #: NP_446295
• N-terminal Sequence: Asp37
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 29-44 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Fc gamma RIIA/CD32a Protein, CF

• CD32 antigen
• CD32a
• CD32MGC23887
• CDw32fc-gamma-RIIa
• Fc fragment of IgG, low affinity IIa, receptor (CD32)
• Fc fragment of IgG, low affinity IIa, receptor for (CD32)
• Fc gamma RIIA
• FCG2
• Fc-gamma RII-a
• Fc-gamma-RIIa
• FcGR
• FCGR2
• FCGR2A
• FCGR2A1
• FcgRIIA
• FCRIIA
• fcRII-a
• IGFR2MGC30032
• IgG Fc receptor II-a
• Immunoglobulin G Fc receptor II
• low affinity immunoglobulin gamma Fc region receptor II-a

Background

Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma  Rs, based on close relationships in their extracellular domains: Fc gamma  RI/CD64, Fc gamma  RII/CD32, and Fc gamma  RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). In humans, there are three Fc gamma  RII genes that encode multiple transcripts that differ in their cytoplasmic tails. Only one murine Fc gamma  RII gene has been identified which has two transcripts that are homologues of the human Fc gamma  RIIB (4). Fc gamma  RIIA is not expressed in mice. Approximately 40 kDa, Fc gamma  RIIA is a type 1 transmembrane glycoprotein that is expressed on different cell types, including, neutrophils, monocytes, dendritic cells, and is the only Fc gamma  R expressed on human platelets (5). The mature protein consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fc gamma RIIA shares 52% amino acid sequence identity with human Fc gamma RIIA. The Fc gamma  RI/CD64 proteins are high affinity receptors (~10^-8-10^-9 M) capable of binding monomeric IgG, whereas the Fc gamma RII/CD32 and Fc gamma  RIII/CD16 proteins bind IgG with lower affinities (~10^-6-10^-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 6). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 7). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (7).

1. Van de Winkel, J. and P. Capel (1993) Immunol. Today. 14:215.
2. Raghavan, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
3. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
4. Ravetch, J.V. and J.P. Kinet (1991) Annu. Rev. Immunol. 9:457.
5. Arman, M. and K. Krauel (2015) J. Thromb. Haemost. 13:893.
6. Takai, T. (2002) Nature Rev. Immunol. 2:580.
7. Ravetch, J. and L. Lanier (2000) Science 290:84.

FAQs for Fc gamma RIIA/CD32a (9696-CD). (Showing 1 - 1 of 1 FAQ).

1. Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
   • For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

Bioinformatics

• Uniprot:
  • NP_446295()