Recombinant Rat CCL20/MIP-3 alpha Protein

• Reactivity: Rt
• Applications: Bioactivity

Recombinant Rat CCL20/MIP-3 alpha Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR6. The ED₅₀ for this effect is 0.5‑3 µg/mL.
• Source: E. coli-derived rat CCL20/MIP-3 alpha protein
  Ala26-Met96
• Accession #: P97884.1
• N-terminal Sequence: Ala26
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ccl20
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CCL20/MIP-3 alpha Protein

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

CCL20, also known as MIP-3 alpha , LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Rat CCL20 cDNA encodes a 96 amino acid (aa) precursor protein with a 25 aa putative signal peptide that is predicted to be cleaved to form the 71 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20 ‑ 28% aa sequence identity). Rat MIP-3 alpha shares approximately 70 and 61% aa sequence homology with mouse and human CCL20, respectively.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly up‑regulated by inflammatory signals and down‑regulated by the anti-inflammatory cytokine IL‑10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR‑L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.

1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
2. Hromas, R. et al. (1997) Blood 89:3315.
3. Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857.
4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Publications for CCL20/MIP-3 alpha (540-RM)(1)

Publication using 540-RM

• HT Lee, SP Liu, CH Lin, SW Lee, CY Hsu, HK Sytwu, CH Hsieh, WC Shyu A Crucial Role of CXCL14 for Promoting Regulatory T Cells Activation in Stroke Theranostics, 2017-02-08;7(4):855-875. 2017-02-08 [PMID: 28382159] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Ccl20
• Uniprot:
  • P97884.1()