Recombinant Mouse TNF-alpha (aa 80-235) Protein

Images

 
There are currently no images for TNF-alpha (410-MT).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse TNF-alpha (aa 80-235) Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.008-0.05 ng/mL.
Source
E. coli-derived mouse TNF-alpha protein
Leu80-Leu235, with an N-terminal Met
Accession #
N-terminal Sequence
Met & Ser84
Structure / Form
A small amount of recombinant protein lacking the N-terminal methionine and four additional amino acid residues may be also present.
Protein/Peptide Type
Recombinant Proteins
Gene
Tnf
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
410-MT in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TNF-alpha (aa 80-235) Protein

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Mouse TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, rat, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Fransen, L. et al. (1985) Nucleic Acids Res. 13:4417.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

Customers Who Viewed This Item Also Viewed...

NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC-P, KO
6507-IL/CF
Species: Hu
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB100-64358
Species: Hu
Applications: Flow, IHC, IHC-P
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
410-MT
Species: Mu
Applications: Bioactivity

Publications for TNF-alpha (410-MT)(201)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 7 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, In vivo, Neutralization, Western Blot.


Filter By Application
Bioassay
(117)
Cell Culture
(2)
ELISA (Standard)
(12)
In Vivo
(61)
In vivo
(2)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(182)
Rat
(1)
N/A
(11)
All Species
Showing Publications 1 - 10 of 201. Show All 201 Publications.
Publications using 410-MT Applications Species
G Zhao, Y Zhong, W Su, S Liu, X Song, T Hou, X Mu, MC Gong, Z Guo Transcriptional suppression of CPI-17 gene expression in vascular smooth muscle cells by TNF, KLF4, and Sp1 is associated with LPS-induced vascular hypocontractility, hypotension, and mortality Mol. Cell. Biol., 2019;0(0):. 2019 [PMID: 30936247] (Bioassay, Mouse) Bioassay Mouse
A Arredouani, A Diane, N Khattab, I Bensmail, I Aoude, M Chikri, R Mohammad, AB Abou-Samra, M Dehbi DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation Sci Rep, 2019;9(1):4772. 2019 [PMID: 30886231] (Bioassay, Mouse) Bioassay Mouse
M Qiu, K Huang, Y Liu, Y Yang, H Tang, X Liu, C Wang, H Chen, Y Xiong, J Zhang, J Yang Modulation of intestinal microbiota by glycyrrhizic acid prevents high-fat diet-enhanced pre-metastatic niche formation and metastasis Mucosal Immunol, 2019;0(0):. 2019 [PMID: 30755716] (Bioassay, Mouse) Bioassay Mouse
P Zizza, R Dinami, M Porru, C Cingolani, E Salvati, A Rizzo, C D'Angelo, E Petti, CA Amoreo, M Mottolese, I Sperduti, A Chambery, R Russo, P Ostano, G Chiorino, G Blandino, A Sacconi, J Cherfils-V, C Leonetti, E Gilson, A Biroccio TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment Nucleic Acids Res., 2019;0(0):. 2019 [PMID: 30698737] (In Vivo, Mouse) In Vivo Mouse
K Paduch, A Debus, B Rai, U Schleicher, C Bogdan Resolution of Cutaneous Leishmaniasis and Persistence of Leishmania major in the Absence of Arginase 1 J. Immunol., 2019;0(0):. 2019 [PMID: 30665936] (Bioassay, Mouse) Bioassay Mouse
AE Vendrov, A Sumida, C Canugovi, A Lozhkin, T Hayami, NR Madamanchi, MS Runge NOXA1-dependent NADPH oxidase regulates redox signaling and phenotype of vascular smooth muscle cell during atherogenesis Redox Biol, 2018;21(0):101063. 2018 [PMID: 30576919] (Bioassay, Mouse) Bioassay Mouse
F Dou, X Chu, B Zhang, L Liang, G Lu, J Ding, S Chen EriB targeted inhibition of microglia activity attenuates MPP+ induced DA neuron injury through the NF-?B signaling pathway Mol Brain, 2018;11(1):75. 2018 [PMID: 30563578] (ELISA (Standard), Mouse) ELISA (Standard) Mouse
A Aguilar-Va, N Haji, D De Gregori, E Matta-Cama, MJ Eslamizade, J Popic, V Sharma, R Cao, C Rummel, A Tanti, S Wiebe, N Nuñez, S Comai, R Nadon, G Luheshi, N Mechawar, G Turecki, JC Lacaille, G Gobbi, N Sonenberg Translational control of depression-like behavior via phosphorylation of eukaryotic translation initiation factor 4E Nat Commun, 2018;9(1):2459. 2018 [PMID: 29941989] (Bioassay, Mouse) Bioassay Mouse
E Archer-Lah, C Lan, RT Jagoe Physiological culture conditions alter myotube morphology and responses to atrophy treatments: implications for in�vitro research on muscle wasting Physiol Rep, 2018;6(12):e13726. 2018 [PMID: 29932505] (Bioassay, Mouse) Bioassay Mouse
I Martins, SQ Raza, L Voisin, H Dakhli, A Allouch, F Law, D Sabino, D De Jong, M Thoreau, E Mintet, D Dugué, M Piacentini, ML Gougeon, F Jaulin, P Bertrand, C Brenner, DM Ojcius, G Kroemer, N Modjtahedi, E Deutsch, JL Perfettini Anticancer chemotherapy and radiotherapy trigger both non-cell-autonomous and cell-autonomous death Cell Death Dis, 2018;9(7):716. 2018 [PMID: 29915308] (Bioassay, Mouse) Bioassay Mouse
Show All 201 Publications.

Reviews for TNF-alpha (410-MT) (0)

There are no reviews for TNF-alpha (410-MT). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for TNF-alpha (410-MT). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Additional TNF-alpha Products

Bioinformatics Tool for TNF-alpha (410-MT)

Discover related pathways, diseases and genes to TNF-alpha (410-MT). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TNF-alpha (410-MT)

Discover more about diseases related to TNF-alpha (410-MT).
 

Pathways for TNF-alpha (410-MT)

View related products by pathway.

PTMs for TNF-alpha (410-MT)

Learn more about PTMs related to TNF-alpha (410-MT).

Blogs on TNF-alpha.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse TNF-alpha (aa 80-235) Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Tnf
Uniprot