Recombinant Mouse TIM-4 Protein, CF

Recombinant Mouse TIM-4 (Catalog # 2826-TI) inhibits anti-CD3-induced proliferation of stimulated human Tcells. Human T cells cultured for 72 hours with PHA are incubated for anadditional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and rmTIM-4.The ED50 for this effect is 0.25-1 µg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TIM-4 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and rmTIM-4.
  The ED₅₀ for this effect is 0.25-1 µg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse TIM-4 protein
  Ala22-Thr279, with a C-terminal 6-His tag
• Accession #: Q6U7R4
• N-terminal Sequence: Ala22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Timd4
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 28.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TIM-4 Protein, CF

• FLJ27515
• Smuckler
• T-cell immunoglobulin and mucin domain containing 4
• T-cell immunoglobulin and mucin domain-containing protein 4
• T-cell membrane protein 4
• TIM4
• TIM-4
• TIMD4
• TIMD-4

Background

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The mouse TIM-4 cDNA encodes a 343 amino acid (aa) precursor that includes a 22 aa signal sequence, a 257 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1, -2, or -3. Within the ECD, mouse TIM-4 shares 27 - 33% aa sequence identity with mouse TIM-1, -2, and -3. The ECD of mouse TIM-4 shares 45% aa sequence identity with that of human and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4⁺ T cells (5, 8). The interaction ofTIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).

1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Publications for TIM-4 (2826-TI)(1)

Publication using 2826-TI

• Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells. Blood, 2007-07-09;110(7):2565-8. 2007-07-09 [PMID: 17620455] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Timd4
• Uniprot:
  • Q6U7R4()