Recombinant Mouse sTNF RI/TNFRSF1A Protein

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse sTNF RI/TNFRSF1A Protein Summary

Details of Functionality
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.1-0.6 µg/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-alpha.
Source
E. coli-derived mouse TNF RI/TNFRSF1A protein
Ile22-Ala212, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Tnfrsf1a
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
425-R1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse sTNF RI/TNFRSF1A Protein

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa extracellular domain (ECD) with a PLAD (pre-ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of mouse TNF RI shares 70%, 88%, 67%, 70% and 64% aa sequence identity with human, rat, canine, feline and porcine TNF RI, respectively. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro-inflammatory stimuli (7). TNF-alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8, 9, 16). Release of the 28-34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10-12). Full-length TNF RI may also be released in exosome-like vesicles (13). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (10, 14, 15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR-associated periodic syndrome) (7).

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Chan, F.K. et al. (2000) Science 288:2351.
  4. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
  5. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  6. Banner, D.W et al. (1993) Cell 73: 431.
  7. Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
  8. Legler, D.F. et al. (2003) Immunity 18:655.
  9. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  10. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  11. Jin, L. et al. (2000) J. Immunol. 165:5153.
  12. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  13. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  14. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  15. McDermott, M.F. et al. (1999) Cell 97:133.

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Publications for TNF RI/TNFRSF1A (425-R1)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), Enzyme Assay, In Vivo, Surface Plasmon Resonance.


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Bioassay
(2)
ELISA (Standard)
(4)
Enzyme Assay
(1)
In Vivo
(3)
Surface Plasmon Resonance
(1)
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Filter By Species
Human
(1)
Mouse
(2)
Rat
(3)
N/A
(5)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 425-R1 Applications Species
D Song, J Meng, J Cheng, Z Fan, P Chen, H Ruan, Z Tu, N Kang, N Li, Y Xu, X Wang, F Shu, L Mu, T Li, W Ren, X Lin, J Zhu, X Fang, MW Amrein, W Wu, LT Yan, J Lü, T Xia, Y Shi Pseudomonas aeruginosa quorum-sensing metabolite induces host immune cell death through cell surface lipid domain dissolution Nat Microbiol, 2018;0(0):. 2018 [PMID: 30510173] (In Vivo, Mouse) In Vivo Mouse
Goodall L, Ovecka M, Rycroft D, Friel S, Sanderson A, Mistry P, Davies M, Stoop A Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension. PLoS ONE, 2015;10(9):e0137065. 2015 [PMID: 26352810] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1. J Biol Chem, 2015;290(7):4022-37. 2015 [PMID: 25538244] (Enzyme Assay, Human) Enzyme Assay Human
Huie JR, Baumbauer KM, Lee KH, Bresnahan JC, Beattie MS, Ferguson AR, Grau JW Glial tumor necrosis factor alpha (TNF-alpha) generates metaplastic inhibition of spinal learning. PLoS ONE, 2012;7(6):e39751. 2012 [PMID: 22745823] (In Vivo, Rat) In Vivo Rat
Mitchell HM, White DM, Domowicz MS, Kraig RP Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by blockade of interleukin-11. J. Neurochem., 2011;117(2):187-96. 2011 [PMID: 21070241] (Bioassay, Rat) Bioassay Rat
Kaneko M, Stellwagen D, Malenka RC, Stryker MP Tumor necrosis factor-alpha mediates one component of competitive, experience-dependent plasticity in developing visual cortex. Neuron, 2008;58(5):673-80. 2008 [PMID: 18549780] (In Vivo, Mouse) In Vivo Mouse
Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins. J. Biol. Chem., 2006;281(41):30684-96. 2006 [PMID: 16882655] (Bioassay, Rat) Bioassay Rat
Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006;177(3):1967-74. 2006 [PMID: 16849510] (ELISA (Standard), N/A) ELISA (Standard) N/A
Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005;302(1):172-81. 2005 [PMID: 16023134] (ELISA (Standard), N/A) ELISA (Standard) N/A
Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2004;231(1):103-11. 2004 [PMID: 15919375] (ELISA (Standard), N/A) ELISA (Standard) N/A
Show All 12 Publications.

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Bioinformatics

Gene Symbol Tnfrsf1a
Uniprot