Recombinant Mouse sTNF RI/TNFRSF1A Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse sTNF RI/TNFRSF1A Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 0.1-0.6 µg/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-alpha .
• Source: E. coli-derived mouse TNF RI/TNFRSF1A protein
  Ile22-Ala212, with an N-terminal Met
• Accession #: P25118
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfrsf1a
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse sTNF RI/TNFRSF1A Protein, CF

• CD120a antigen
• CD120a
• FPF
• p55
• p55-R
• p60
• TNF RI
• TNFARMGC19588
• TNF-R
• TNF-R1
• TNFR1TBP1
• TNFR55
• TNF-R55
• TNFR60
• TNFRI
• TNF-RI
• TNF-R-I
• TNFR-I
• TNFRSF1A
• tumor necrosis factor binding protein 1
• Tumor necrosis factor receptor 1
• tumor necrosis factor receptor 1A isoform beta
• tumor necrosis factor receptor superfamily member 1A
• tumor necrosis factor receptor superfamily, member 1A
• tumor necrosis factor receptor type 1
• Tumor necrosis factor receptor type I
• tumor necrosis factor-alpha receptor

Background

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa extracellular domain (ECD) with a PLAD (pre-ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of mouse TNF RI shares 70%, 88%, 67%, 70% and 64% aa sequence identity with human, rat, canine, feline and porcine TNF RI, respectively. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro-inflammatory stimuli (7). TNF-alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8, 9, 16). Release of the 28-34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10-12). Full-length TNF RI may also be released in exosome-like vesicles (13). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (10, 14, 15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR-associated periodic syndrome) (7).

1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
3. Chan, F.K. et al. (2000) Science 288:2351.
4. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
5. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
6. Banner, D.W et al. (1993) Cell 73: 431.
7. Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
8. Legler, D.F. et al. (2003) Immunity 18:655.
9. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
10. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
11. Jin, L. et al. (2000) J. Immunol. 165:5153.
12. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
13. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
14. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
15. McDermott, M.F. et al. (1999) Cell 97:133.

Publications for TNF RI/TNFRSF1A (425-R1/CF)(16)

Publications using 425-R1/CF

• D Kleidonas, A Vlachos Scavenging Tumor Necrosis Factor alpha Does Not Affect Inhibition of Dentate Granule Cells Following In Vitro Entorhinal Cortex Lesion Cells, 2021-11-19;10(11):. 2021-11-19 [PMID: 34831454] (Bioassay, Mouse)
• C Dykstra-Ai, KMS Koh, J Nguyen, M Xue, S Roy, JM Krueger A wake-like state in vitro induced by transmembrane TNF/soluble TNF receptor reverse signaling Brain, Behavior, and Immunity, 2021-02-09;0(0):. 2021-02-09 [PMID: 33571627] (Bioassay, Mouse)
• JJ Muldoon, Y Chuang, N Bagheri, JN Leonard Macrophages employ quorum licensing to regulate collective activation Nat Commun, 2020-02-13;11(1):878. 2020-02-13 [PMID: 32054845] (Bioassay, Mouse)
• D Song, J Meng, J Cheng, Z Fan, P Chen, H Ruan, Z Tu, N Kang, N Li, Y Xu, X Wang, F Shu, L Mu, T Li, W Ren, X Lin, J Zhu, X Fang, MW Amrein, W Wu, LT Yan, J Lü, T Xia, Y Shi Pseudomonas aeruginosa quorum-sensing metabolite induces host immune cell death through cell surface lipid domain dissolution Nat Microbiol, 2018-12-03;0(0):. 2018-12-03 [PMID: 30510173] (In Vivo, Mouse)
• Goodall L, Ovecka M, Rycroft D, Friel S, Sanderson A, Mistry P, Davies M, Stoop A Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension. PLoS ONE, 2015-09-09;10(9):e0137065. 2015-09-09 [PMID: 26352810] (Surface Plasmon Resonance)
• Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1. J Biol Chem, 2014-12-23;290(7):4022-37. 2014-12-23 [PMID: 25538244] (Enzyme Assay, Human)
• Freeman B, Meyer C, Slifka M Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions. J Virol, 2014-04-16;88(13):7474-84. 2014-04-16 [PMID: 24741101] (Bioassay, Mouse)
• Huie JR, Baumbauer KM, Lee KH, Bresnahan JC, Beattie MS, Ferguson AR, Grau JW Glial tumor necrosis factor alpha (TNF-alpha) generates metaplastic inhibition of spinal learning. PLoS ONE, 2012-06-20;7(6):e39751. 2012-06-20 [PMID: 22745823] (In Vivo, Rat)
• Mitchell HM, White DM, Domowicz MS, Kraig RP Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by blockade of interleukin-11. J. Neurochem., 2010-12-01;117(2):187-96. 2010-12-01 [PMID: 21070241] (Bioassay, Rat)
• Kaneko M, Stellwagen D, Malenka RC, Stryker MP Tumor necrosis factor-alpha mediates one component of competitive, experience-dependent plasticity in developing visual cortex. Neuron, 2008-06-12;58(5):673-80. 2008-06-12 [PMID: 18549780] (In Vivo, Mouse)
• Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins. J. Biol. Chem., 2006-08-01;281(41):30684-96. 2006-08-01 [PMID: 16882655] (Bioassay, Rat)
• Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA (Standard))
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA (Standard))
• Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2005-02-26;231(1):103-11. 2005-02-26 [PMID: 15919375] (ELISA (Standard))
• Lambertsen KL, Gregersen R, Meldgaard M, Clausen BH, Heibol EK, Ladeby R, Knudsen J, Frandsen A, Owens T, Finsen B A role for interferon-gamma in focal cerebral ischemia in mice. J. Neuropathol. Exp. Neurol., 2004-09-01;63(9):942-55. 2004-09-01 [PMID: 15453093] (In Vivo, Mouse)
• LaBue M, Colburn KK, Green LM Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity. Thyroid, 2004-04-01;14(4):249-62. 2004-04-01 [PMID: 15142358] (ELISA (Standard))

Bioinformatics

• Gene Symbol: Tnfrsf1a
• Uniprot:
  • P25118()