Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF

Images

 
Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater ...read more
1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.05-0.25 µg/mL.
Source
E. coli-derived mouse Sonic Hedgehog/Shh protein
Cys25-Gly198 (Cys25Ile-Ile), with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Shh
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
464-SH/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • ShhNC
  • SMMCI
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Echelard, Y. et al. (1993) Cell 75:1417.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

Customers Who Viewed This Item Also Viewed...

NB600-600
Species: Hu, Mu, Ca
Applications: WB, ChIP, ELISA, Flow, IB, IHC, IHC-P, Flow-IC, KD
NBP1-71662
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00003077-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NLS2666
Species: Hu, Mu, Rt, Po, Bt, Bv, Ca, Eq, Ha, Pm, Rb
Applications: ICC/IF, IHC, IHC-P
NBP2-55775
Species: Hu
Applications: WB, ICC/IF
NLS2666
Species: Hu, Mu, Rt, Po, Bt, Bv, Ca, Eq, Ha, Pm, Rb
Applications: ICC/IF, IHC, IHC-P
AF3690
Species: Hu, Mu
Applications: WB, ChIP, ICC
NB600-874
Species: Hu, Mu, Rt
Applications: WB, ChIP, ELISA, IHC, IHC-P, Flow-IC
AF482
Species: Mu
Applications: WB, IHC
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-80532
Species: Hu, Pm
Applications: WB, IHC, IHC-P
7460-F4/CF
Species: Hu
464-SH/CF
Species: Mu
Applications: Bioactivity

Publications for Sonic Hedgehog/Shh (464-SH/CF)(39)

We have publications tested in 4 confirmed species: Human, Mouse, Chicken, Zebrafish.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Tissue Culture.


Filter By Application
Bioassay
(32)
Cell Culture
(4)
Differentiation
(1)
In Vivo
(1)
Tissue Culture
(1)
All Applications
Filter By Species
Human
(21)
Mouse
(15)
Chicken
(1)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using 464-SH/CF Applications Species
J Aldaregia, P Errarte, A Olazagoiti, M Gimeno, JJ Uriz, TR Gershon, I Garcia, A Matheu Erbb4 Is Required for Cerebellar Developmentand Malignant Phenotype of Medulloblastoma Cancers (Basel), 2020;12(4):. 2020 [PMID: 32316671] (Bioassay, Mouse) Bioassay Mouse
S Kasiri, B Chen, AN Wilson, A Reczek, S Mazambani, J Gadhvi, E Noel, U Marriam, B Mino, W Lu, L Girard, LM Solis, K Luby-Phelp, J Bishop, JW Kim, J Kim Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species Oncogene, 2020;39(16):3258-3275. 2020 [PMID: 32108165] (Bioassay, Human) Bioassay Human
Y Wada, A Kikuchi, A Kaga, N Shimizu, J Ito, R Onuma, F Fujishima, E Totsune, R Sato, T Niihori, M Shirota, R Funayama, K Sato, T Nakazawa, K Nakayama, Y Aoki, S Aiba, K Nakagawa, S Kure Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice PLoS Genet., 2020;16(2):e1008628. 2020 [PMID: 32101538] (Cell Culture, Mouse) Cell Culture Mouse
S Mahajani, A Raina, C Fokken, S Kügler, M Bähr Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors Cell Death Dis, 2019;10(12):898. 2019 [PMID: 31776327] (Cell Culture, Human) Cell Culture Human
D Leitner, M Ramamoorth, M Dejosez, TP Zwaka Immature mDA neurons ameliorate motor deficits in a 6-OHDA Parkinson&#039;s disease mouse model and are functional after cryopreservation Stem Cell Res, 2019;41(0):101617. 2019 [PMID: 31731178] (Cell Culture, Human) Cell Culture Human
IR de Luzy, JC Niclis, CW Gantner, JA Kauhausen, CPJ Hunt, C Ermine, CW Pouton, LH Thompson, CL Parish Isolation of LMX1a ventral midbrain progenitors improves the safety and predictability of human pluripotent stem cell-derived neural transplants in Parkinsonian Disease J. Neurosci., 2019;0(0):. 2019 [PMID: 31641054] (Bioassay, Human) Bioassay Human
M Riessland, B Kolisnyk, TW Kim, J Cheng, J Ni, JA Pearson, EJ Park, K Dam, D Acehan, LS Ramos-Espi, W Wang, J Zhang, JW Shim, G Ciceri, L Brichta, L Studer, P Greengard Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons Cell Stem Cell, 2019;25(4):514-530.e8. 2019 [PMID: 31543366] (Bioassay, Human) Bioassay Human
C Penas, ME Maloof, V Stathias, J Long, SK Tan, J Mier, Y Fang, C Valdes, J Rodriguez-, CM Chiang, DJ Robbins, DJ Liebl, JK Lee, ME Hatten, J Clarke, NG Ayad Time series modeling of cell cycle exit identifies Brd4 dependent regulation of cerebellar neurogenesis Nat Commun, 2019;10(1):3028. 2019 [PMID: 31292434] (Cell Culture, Mouse) Cell Culture Mouse
Y Xiang, Y Tanaka, B Cakir, B Patterson, KY Kim, P Sun, YJ Kang, M Zhong, X Liu, P Patra, SH Lee, SM Weissman, IH Park hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids Cell Stem Cell, 2019;0(0):. 2019 [PMID: 30799279] (Bioassay, Mouse) Bioassay Mouse
S Grabow, AJ Kueh, F Ke, HK Vanyai, BN Sheikh, MA Dengler, W Chiang, S Eccles, IM Smyth, LK Jones, FJ de Sauvage, M Scott, L Whitehead, AK Voss, A Strasser Subtle Changes in the Levels of BCL-2 Proteins Cause Severe Craniofacial Abnormalities Cell Rep, 2018;24(12):3285-3295.e4. 2018 [PMID: 30232009] (Bioassay, Mouse) Bioassay Mouse
Show All 39 Publications.

Reviews for Sonic Hedgehog/Shh (464-SH/CF) (0)

There are no reviews for Sonic Hedgehog/Shh (464-SH/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Sonic Hedgehog/Shh (464-SH/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Sonic Hedgehog/Shh Products

Bioinformatics Tool for Sonic Hedgehog/Shh (464-SH/CF)

Discover related pathways, diseases and genes to Sonic Hedgehog/Shh (464-SH/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Sonic Hedgehog/Shh (464-SH/CF)

Discover more about diseases related to Sonic Hedgehog/Shh (464-SH/CF).
 

Pathways for Sonic Hedgehog/Shh (464-SH/CF)

View related products by pathway.

PTMs for Sonic Hedgehog/Shh (464-SH/CF)

Learn more about PTMs related to Sonic Hedgehog/Shh (464-SH/CF).

Blogs on Sonic Hedgehog/Shh

There are no specific blogs for Sonic Hedgehog/Shh, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GLI-1 Antibody
NB600-600

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Shh
Uniprot