Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF


Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater more
1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.

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Applications Bioactivity

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Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.05-0.25 µg/mL.
E. coli-derived mouse Sonic Hedgehog/Shh protein
Cys25-Gly198 (Cys25Ile-Ile), with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
464-SH/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • ShhNC
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT


Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Echelard, Y. et al. (1993) Cell 75:1417.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

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Publications for Sonic Hedgehog/Shh (464-SH/CF)(47)

We have publications tested in 6 confirmed species: Human, Mouse, Chicken, Primate - Rhesus macaque, Simian, Zebrafish.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Tissue Culture.

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Showing Publications 1 - 10 of 47. Show All 47 Publications.
Publications using 464-SH/CF Applications Species
AM Earley, LF Burbulla, D Krainc, R Awatramani Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neurons Scientific Reports, 2021;11(1):22257. 2021 [PMID: 34782629] (Bioassay, Human) Bioassay Human
IR de Luzy, KCL Law, N Moriarty, CPJ Hunt, JC Durnall, LH Thompson, A Nagy, CL Parish Human stem cells harboring a suicide gene improve the�safety and standardisation of neural transplants in Parkinsonian rats Nature Communications, 2021;12(1):3275. 2021 [PMID: 34045451] (Bioassay, Human) Bioassay Human
V Padmanabha, H Liu, G Ciceri, J Jungverdor, G Frishman, J Tchieu, GY Cederquist, I Rothenaign, K Schorpp, L Klepper, RM Walsh, TW Kim, D Cornacchia, A Ruepp, J Mayer, K Hadian, D Frishman, L Studer, M Vincendeau Activation of HERV-K(HML-2) disrupts cortical patterning and neuronal differentiation by increasing NTRK3 Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33951478] (Bioassay, Human) Bioassay Human
Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys Nature Medicine, 2021;0(0):. 2021 [PMID: 33649496] (Bioassay, Primate - Rhesus macaque) Bioassay Primate - Rhesus macaque
J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01 Cell Stem Cell, 2021;28(2):217-229.e7. 2021 [PMID: 33545080] (Bioassay, Human) Bioassay Human
JW Kim, X Yin, A Jhaldiyal, MR Khan, I Martin, Z Xie, T Perez-Rose, M Kumar, L Abalde-Atr, J Xu, L Chen, SM Eacker, DJ Surmeier, NT Ingolia, TM Dawson, VL Dawson Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis Cell Stem Cell, 2020;27(4):633-645.e7. 2020 [PMID: 32846140] (Bioassay, Human) Bioassay Human
J Aldaregia, P Errarte, A Olazagoiti, M Gimeno, JJ Uriz, TR Gershon, I Garcia, A Matheu Erbb4 Is Required for Cerebellar Developmentand Malignant Phenotype of Medulloblastoma Cancers (Basel), 2020;12(4):. 2020 [PMID: 32316671] (Bioassay, Mouse) Bioassay Mouse
S Kasiri, B Chen, AN Wilson, A Reczek, S Mazambani, J Gadhvi, E Noel, U Marriam, B Mino, W Lu, L Girard, LM Solis, K Luby-Phelp, J Bishop, JW Kim, J Kim Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species Oncogene, 2020;39(16):3258-3275. 2020 [PMID: 32108165] (Bioassay, Human) Bioassay Human
SC Vermilyea, A Babinski, N Tran, S To, S Guthrie, JH Kluss, JK Schmidt, GJ Wiepz, MG Meyer, ME Murphy, MR Cookson, ME Emborg, TG Golos In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson&#039;s Disease in Common Marmosets Sci Rep, 2020;10(1):3447. 2020 [PMID: 32103062] (Cell Culture, Simian) Cell Culture Simian
Y Wada, A Kikuchi, A Kaga, N Shimizu, J Ito, R Onuma, F Fujishima, E Totsune, R Sato, T Niihori, M Shirota, R Funayama, K Sato, T Nakazawa, K Nakayama, Y Aoki, S Aiba, K Nakagawa, S Kure Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice PLoS Genet., 2020;16(2):e1008628. 2020 [PMID: 32101538] (Cell Culture, Mouse) Cell Culture Mouse
Show All 47 Publications.

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Gene Symbol Shh