Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein, CF

When Recombinant Human Plexin B2 (Catalog # 5329-PB) is coated at 5 μg/mL, 100 μL/well, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera (Catalog # 5235-S4B) binds with an ED50 of 4-24 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 (Catalog # 5329-PB) is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera binds with an ED₅₀ of 4-24 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Semaphorin 4D/CD100 protein
  

  Mouse Semaphorin 4D/CD100 (Phe24-Met711) Accession # NP_038688.2	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: NP_038688.2
• N-terminal Sequence: Phe24
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW:

  103 kDa

  .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-135 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein, CF

• A8
• BB18
• CD100 antigen
• CD100
• CD100M-sema-G
• COLL-4
• FLJ33485
• FLJ34282
• FLJ39737
• FLJ46484
• immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
• MGC169138
• MGC169141
• M-sema-G
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
• SEMA4D
• SEMAJ
• Semaphorin 4D
• semaphorin-4D

Background

Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a 150 kDa type I transmembrane glycoprotein of the Class 4 family of transmembrane immune and nervous system semaphorins (1-3). The mouse Sema4D cDNA encodes 861 amino acids (aa) including a 23 aa signal sequence, a 710 aa extracellular domain (ECD) with sema, PSI and immunoglobulin-like domains, a 21 aa transmembrane domain and a 107 aa cytoplasmic domain. Within the ECD portion expressed (aa 24-711), mouse Sema4D shares 93%, 87%, 82% and 78% aa identity with rat, human, porcine and canine Sema4D, respectively. Sema4D is active as a homodimer that occurs via a membrane-proximal intermolecular disulfide (C707) and hydrophobic interactions involving F244 and F246 (4, 5). Proteolysis by metalloproteinases such as TACE/ADAM17 and MMP-14 (MT1-MMP) produces a soluble, active 120 kDa form that is also active as a dimer (5-7). Sema4D, produced by T cells and activated B and dendritic cells, acts through its low affinity receptor CD72 in the immune system (1, 2). CD72 inhibits the antigen presenting cells that express it; this inhibition is relieved by Sema4D binding (8). Sema4D thus causes enhanced B cell survival, differentiation of macrophages and DC, and enhanced T cell antigen priming (2, 8, 9). Sema4D and its high affinity receptor, Plexin B1, are expressed in complimentary patterns in several regions of the brain, and assist in guidance of developing neurons (10). Lower-affinity Sema4D/Plexin B2 binding also enhances neuronal radial and tangential migration (11). Sema4D produced by tumor-associated macrophages binds endothelial cell Plexin B1 to promote tumor angiogenesis, while Sema4D-induced neovascularization and platelet activation is involved in atherosclerosis (3, 6, 7, 12-14). Receptors associated in cis with plexins, such as Met and ErbB2, can alter Sema4D-mediated activities (3).

1. Furuyama, T. et al. (1996) J. Biol. Chem. 271:33376.
2. Kumanogoh, A. and H. Kikutani (2004) Cell. Mol. Life Sci. 61:292.
3. Ch’ng, E.S. and A. Kumanogoh (2010) Mol. Cancer 9:251.
4. Janssen, B.J.C. et al. (2010) Nature 467:1118.
5. Elhabazi, A. et al. (2001) J. Immunol. 166:4341.
6. Zhu, L. et al. (2007) Proc. Natl. Acad. Sci. USA 104:1621.
7. Basile, J.R. et al. (2007) J. Biol. Chem. 282:6899.
8. Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
9. Ishida, I. et al. (2003) Int. Immunol. 15:1027.
10. Worzfield, T. et al. (2004) Eur. J. Neurosci. 19:2622.
11. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
12. Sierra, J.R. et al. (2008) J. Exp. Med. 205:1673.
13. Yukawa, K. et al. (2010) Int. J. Mol. Med. 26:39.
14. Zhu, L. et al. (2009) Atheroscler. Thromb. Vasc. Biol. 29:1039.

Publications for Semaphorin 4D/CD100 (5235-S4B)(3)

Publications using 5235-S4B

• N Vilallongu, J Schaeffer, AM Hesse, C Delpech, B Blot, A Paccard, E Plissonnie, B Excoffier, Y Couté, S Belin, H Nawabi Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system Nature Communications, 2022-10-13;13(1):6040. 2022-10-13 [PMID: 36229455] (Bioassay, Mouse)
• A Mediero, T Wilder, L Shah, BN Cronstein Adenosine Areceptor (A2AR) stimulation modulates expression of semaphorins 4D and 3A, regulators of bone homeostasis FASEB J., 2018-02-02;0(0):fj201700217R. 2018-02-02 [PMID: 29394106] (Differentiation, Differentiation, Mouse)
• Zielonka M, Krishnan R, Swiercz J, Offermanns S The IkappaB kinase complex is required for plexin-B-mediated activation of RhoA. PLoS ONE, 2014-08-19;9(8):e105661. 2014-08-19 [PMID: 25137062] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • NP_038688.2()