![Western Blot: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Western-Blot-NBP2-73857-img0001.jpg "Western Blot: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC, CyTOF-ready |
| Clone | OTI2B2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-234 of human RFC2(NP_002905) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RFC2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 35.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A2 Antibody [NBP1-59069] - overexpressed mouse sequences tissue](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Immunohistochemistry-Paraffin-NBP1-59069-img0005.jpg)
![Western Blot: GPHA2 Antibody [NBP3-17255] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/images2/GPHA2-Antibody-Western-Blot-NBP3-17255-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GPHA2 Antibody [NBP3-17255] - Staining of human cell line Hep G2 shows localization to vesicles.](http://images.novusbio.com/images2/GPHA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17255-img0001.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HNRNPC antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(CL2593)-Western-Blot-NBP2-36776-img0015.jpg)
![Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human rectum shows strong nuclear immunoreactivity in glandular and lamina propria cells.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(CL2593)-Immunohistochemistry-NBP2-36776-img0001.jpg)
![Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human small intestine shows strong nuclear positivity in glandular epithelium and in lamina propria cells.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(CL2593)-Immunohistochemistry-NBP2-36776-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/images2/SPANXB1-Antibody-Immunocytochemistry-Immunofluorescence-H00728695-B01P-img0003.jpg)
![Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/SPANXB1-Antibody-Western-Blot-H00728695-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human cerebral cortex shows moderate postivity in neuropil.](http://images.novusbio.com/images2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0005.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human fallopian tube shows moderate cytoplasmic postivity in glandular cells.](http://images.novusbio.com/images2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0006.jpg)
![Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human placenta shows moderate granular cytoplasmic postivity in trophoblastic cells.](http://images.novusbio.com/images2/PLEKHM1-Antibody-Immunohistochemistry-Paraffin-NBP2-38449-img0007.jpg)
![Western Blot: ARHGAP4 Antibody [NBP1-88527] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/images2/ARHGAP4-Antibody-Western-Blot-NBP1-88527-img0009.jpg)
![Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/images2/ARHGAP4-Antibody-Immunohistochemistry-Paraffin-NBP1-88527-img0010.jpg)
![Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human spleen shows high expression.](http://images.novusbio.com/images2/ARHGAP4-Antibody-Immunohistochemistry-Paraffin-NBP1-88527-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PFDN4 Antibody [NBP2-93935] - Analysis of L929 cells using PFDN4 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/PFDN4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93935-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PFDN4 Antibody [NBP2-93935] - Analysis of U-2 OS cells using PFDN4 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/PFDN4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93935-img0002.jpg)
![Western Blot: PFDN4 Antibody [NBP2-93935] - Analysis of extracts of various cell lines, using PFDN4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/images2/PFDN4-Antibody-Western-Blot-NBP2-93935-img0006.jpg)
![Immunohistochemistry-Frozen: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review.](http://images.novusbio.com/images2/beta-Amyloid-Antibody-(MOAB-2)-Immunohistochemistry-Frozen-NBP2-13075-img0018.jpg)
![Immunohistochemistry: beta Amyloid Antibody (MOAB-2) - BSA Free [NBP2-13075] - A plaque load in neocortex and hippocampus in APP/PS1 mice exposed to differential housing. Representative 10x MOAB-2 staining in hippocampus of APP/PS1 mice for each housing group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28584278/) licensed under a CC-BY licence.](http://images.novusbio.com/images2/beta-Amyloid-Antibody-(MOAB-2)-Immunohistochemistry-NBP2-13075-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).](http://images.novusbio.com/images2/beta-Amyloid-Antibody-(MOAB-2)-Immunocytochemistry-Immunofluorescence-NBP2-13075-img0009.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human kidney shows high expression.](http://images.novusbio.com/images2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0005.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/images2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A4 Antibody [NBP1-89330] - Staining in human kidney and lymph node tissues using anti-ATP6V0A4 antibody. Corresponding ATP6V0A4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/ATP6V0A4-Antibody-Immunohistochemistry-Paraffin-NBP1-89330-img0007.jpg)
![Immunohistochemistry: PLP2 Antibody [NBP3-06290] - Immunochemical staining of human PLP2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/images2/PLP2-Antibody-Immunohistochemistry-NBP3-06290-img0001.jpg)
![Immunohistochemistry-Paraffin: PLP2 Antibody [NBP3-06290] - Immunochemical staining of human PLP2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/images2/PLP2-Antibody-Immunohistochemistry-Paraffin-NBP3-06290-img0002.jpg)
![ELISA: Dopamine D2R/DRD2 Antibody [NBP2-88996] - HEK293 cells expressing dopamine receptor (D2) were treated with indicated concentrations of agonist (Quinpirol) and antagonist (Siperone) and probed with Dopamine Receptor (D2) pAb (1:1000 of a stock solution of 1ug/uL) by cellular ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean -+SEM (n=3).](http://images.novusbio.com/images2/Dopamine-D2R-DRD2-Antibody-ELISA-NBP2-88996-img0001.jpg)
![Immunohistochemistry: Dopamine D2R/DRD2 Antibody [NBP2-88996] - Rat cerebral cortex. Neurons are labeled with Dopamine Receptor (D2) pAb in conjunction with Cy3 conjugated rabbit IgG antibody (H+L).](http://images.novusbio.com/images2/Dopamine-D2R-DRD2-Antibody-Immunohistochemistry-NBP2-88996-img0002.jpg)
![Western Blot: Dopamine D2R/DRD2 Antibody [NBP2-88996] - MW marker (1) and 10ug of rat heart membrane extract (2) probed with Dopamine Receptor (D2) pAb at 4.0ug/ml.](http://images.novusbio.com/images2/Dopamine-D2R-DRD2-Antibody-Western-Blot-NBP2-88996-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for RFC2 Antibody (NBP2-73857)Discover more about diseases related to RFC2 Antibody (NBP2-73857).
| Pathways for RFC2 Antibody (NBP2-73857)View related products by pathway.
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PTMs for RFC2 Antibody (NBP2-73857)Learn more about PTMs related to RFC2 Antibody (NBP2-73857).
| Research Areas for RFC2 Antibody (NBP2-73857)Find related products by research area.
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| Gene Symbol | RFC2 |
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0012.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human prostate tissue.")
![Flow Cytometry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RFC2 antibody.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-73857-img0002.jpg "Flow Cytometry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RFC2 antibody.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0003.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Adenocarcinoma of Human endometrium tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0004.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Adenocarcinoma of Human ovary tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0005.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Carcinoma of Human prostate tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0006.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Carcinoma of Human thyroid tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0007.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human breast tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0008.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human Kidney tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0009.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human lymphoma tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0010.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human Ovary tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0011.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human pancreas tissue.")
![Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(OTI2B2)-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73857-img0013.jpg "Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human tonsil tissue.")
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0002.jpg)
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TCIRG1 Antibody [NBP1-89333] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/TCIRG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89333-img0007.jpg)
![Immunohistochemistry-Paraffin: TCIRG1 Antibody [NBP1-89333] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/images2/TCIRG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89333-img0006.jpg)
![Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/images2/BBS9-Antibody-Immunohistochemistry-Paraffin-NBP1-88927-img0002.jpg)
![Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.](http://images.novusbio.com/images2/MS4A1-CD20-Antibody-(AISB12)-Western-Blot-NBP1-43435-img0002.jpg)
![Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/images2/MS4A1-CD20-Antibody-(AISB12)-Flow-Cytometry-NBP1-43435-img0004.jpg)
![Immunohistochemistry-Paraffin: gamma Sarcoglycan Antibody [NBP1-90299] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/images2/gamma-Sarcoglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-90299-img0007.jpg)
![Immunohistochemistry-Paraffin: gamma Sarcoglycan Antibody [NBP1-90299] - Staining in human heart muscle and prostate tissues using anti-SGCG antibody. Corresponding SGCG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/gamma-Sarcoglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-90299-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Sarcoglycan Antibody [NBP1-90299] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/gamma-Sarcoglycan-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90299-img0010.jpg)
![Complement Component C2 Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Complement Component C2 Antibody [Unconjugated]")
![Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-1/ApoA1 Antibody NBP2-52979 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .](http://images.novusbio.com/images2/Apolipoprotein-A-I-ApoA1-Antibody-Flow-Cytometry-NBP2-52979-img0004.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Western blot analysis using Apolipoprotein A-I/ApoA1 antibody. Harvested from mouse, separated on a 4-12 gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 3% BSA/TBST. Probed with 1:8000 anti-apoA1 in 1%BSA/TBST, and detect edwith an anti-rabbit HRP secondary antibody using chemiluminescence. WB dilution 1: 8000, block before incubation overnight. Image from verified customer review.](http://images.novusbio.com/images2/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NBP2-52979-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP2-52979] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Apolipoprotein A-1/ApoA1 Antibody (NBP2-52979) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/images2/Apolipoprotein-A-I-ApoA1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0006.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-(MG1)-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")