Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF

Recombinant Mouse S100A8/S100A9 (Catalog #8916-S8) induces IL-6 secretion by A375 human melanoma cells. The ED50 for this effect is1.5-6 μg/mL.

1 μg/lane of Recombinant Mouse S100A8/S100A9 was resolved withSDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by silver staining, showing R bands at6.3, 10.8 kDa and NR band at 6, 9.6 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF Summary

• Details of Functionality: Measured by its ability to induce IL-6 secretion by A375 human melanoma cells. Hibino, T. et al. (2013) Cancer Res. 73:172. The ED₅₀ for this effect is 1.5-6 μg/mL.
• Source: E. coli-derived mouse S100A8/S100A9 Heterodimer protein
  

  Mouse S100A8 (Pro2-Glu89) Accession # P27005

  Mouse S100A9 (Ala2-Lys113) Accession # P31725
  N-terminus	C-terminus

• N-terminal Sequence: Pro2 (S100A8), Ala2 (S100A9)
• Structure / Form: Non-covalent heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 10 kDa (S100A8), 13 kDa (S100A9).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 7 kDa and 12 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF

• 60B8AG
• CAGA
• calgranulin A
• Calprotectin L1L subunit
• Calprotectin
• CFAG
• CGLA
• CP-10
• Cystic fibrosis antigen
• L1AG
• Leukocyte L1 complex light chain
• MA387
• MIF
• Migration inhibitory factor-related protein 8
• MRP8
• NIF
• p8
• protein S100-A8
• S100-A8
• S100A8/S100A9 Heterodimer
• Urinary stone protein band A

Background

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). The 10 kDa S100A8 and 14 kDa S100A9 each contain two EF-hand calcium binding motifs. Mouse S100A8 shares 57% and 80% amino acid (aa) sequence identity with human and rat S100A8, respectively. Mouse S100A9 shares 57% and 79% amino acid sequence identity with human and rat S100A9, respectively (3, 4). S100A8 and S100A9 can associate into non-covalent homodimers and 34-35 kDa heterodimers known as S100A8/9 or Calprotectin. The presence of calcium and zinc also induces the formation of the tetramer (5, 6). S100A8 and S100A9 are up-regulated in neutrophils, monocytes, Schwann cells, and keratinocytes at sites of inflammation (7-13). The S100A8/9 heterodimer is elevated in rheumatoid arthritis synovial fluid and in the serum of cardiovascular disease, atopic dermatitis, and psoriatic arthritis patients (1, 9, 13-15). It exerts its effects through the receptors RAGE and TLR4 (11, 16). The heterodimer promotes neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (5, 8, 9, 12). It promotes astrocyte proliferation (16) and the suppression of tumor growth by promoting the influx and activation of NK cells (10, 11). The S100A8/9 heterodimer additionally binds to unsaturated fatty acids such as arachidonic acid (7), and it sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (17).

1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
2. Kerkhoff, C. et al. (2012) Exp. Dermatol. 21:822.
3. Lagasse, E. and I.L. Weissman (1992) Blood 79:1907.
4. Lackmann, M. et al. (1993) J. Immunol. 150:2981.
5. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
6. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
7. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
8. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
9. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
10. Zhang, L. et al. (2015) Mol. Med. Rep. 11:4093.
11. Narumi, K. et al. (2015) J. Immunol. 194:5539.
12. Chernov, A.V. et al. (2015) J. Biol. Chem. 290:11771.
13. Jin, S. et al. (2014) Exp. Dermatol. 23:938.
14. Vogl, T. et al. (2014) Nat. Commun. 5:4593.
15. Hansson, C. et al. (2014) J. Immunol. Res. 2014:696415.
16. Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.
17. Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841.

Publications for S100A8/S100A9 Heterodimer (8916-S8)(7)

Publications using 8916-S8

• Franz, S;Torregrossa, M;Anderegg, U;Ertel, A;Saalbach, A; Dysregulated S100A9 Expression Impairs Matrix Deposition in Chronic Wounds International journal of molecular sciences 2024-09-16 [PMID: 39337466] (Bioassay, Mouse)
• Chen, J;Chen, C;Wang, L;Feng, X;Chen, Y;Zhang, R;Cheng, Y;Liu, Z;Chen, Q; Identification of S100A8/A9 involved in thromboangiitis obliterans development using tandem mass tags-labeled quantitative proteomics analysis Cellular signalling 2024-04-30 [PMID: 38697446] (Bioassay, Rat)
• N Maehara, K Taniguchi, A Okuno, H Ando, A Hirota, Z Li, CT Wang, S Arai, T Miyazaki AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke Cell Reports, 2021-09-14;36(11):109693. 2021-09-14 [PMID: 34525359] (Bioassay, Human)
• M Wu, L Xu, Y Wang, N Zhou, F Zhen, Y Zhang, X Qu, H Fan, S Liu, Y Chen, R Yao S100A8/A9 induces microglia activation and promotes the apoptosis of oligodendrocyte precursor cells by activating the NF-?B signaling pathway Brain Res. Bull., 2018-09-25;0(0):. 2018-09-25 [PMID: 30266587] (Bioassay, Mouse)
• H Gong, WJ Su, ZY Cao, YJ Lian, W Peng, YZ Liu, Y Zhang, LL Liu, R Wu, B Wang, T Zhang, YX Wang, CL Jiang Hippocampal Mrp8/14 signaling plays a critical role in the manifestation of depressive-like behaviors in mice J Neuroinflammation, 2018-09-04;15(1):252. 2018-09-04 [PMID: 30180864] (In Vivo, Mouse)
• A Basu, S Munir, MA Mulaw, K Singh, B Herold, D Crisan, A Sindrilaru, N Treiber, M Wlaschek, M Huber-Lang, F Gebhard, K Scharffett A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing Sci Rep, 2018-04-18;8(1):6205. 2018-04-18 [PMID: 29670130] (Bioassay, Mouse)
• S Son, I Hwang, SH Han, JS Shin, OS Shin, JW Yu Advanced glycation end products impair NLRP3 inflammasome-mediated innate immune responses in macrophages J. Biol. Chem., 2017-10-19;0(0):. 2017-10-19 [PMID: 29051224] (Bioassay, Mouse)