Recombinant Mouse S100A8 Protein, CF

Recombinant Mouse S100A8 (Catalog # 9877-S8) inducesCXCL1/KC secretion in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 forthis effect is 1.5-9 μg/mL.

1 μg/lane of Recombinant Mouse S100A8 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 7 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse S100A8 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.5-9 μg/mL.
• Source: E. coli-derived mouse S100A8 protein
  Met1-Glu89
• Accession #: P27005
• N-terminal Sequence: Met1 & Pro2
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 7 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and TCEP with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse S100A8 Protein, CF

• 60B8AG
• CAGACP-10
• Calgranulin A
• calgranulin-A
• Calprotectin L1L subunit
• CFAG
• CFAGL1Ag
• CGLA
• Cystic fibrosis antigen
• Leukocyte L1 complex light chain
• MA387
• MIF
• Migration inhibitory factor-related protein 8
• MRP8
• MRP-8
• MRP8S100 calcium binding protein A8 (calgranulin A)
• NIF
• P8
• protein S100-A8
• S100 calcium binding protein A8
• S100 calcium-binding protein A8 (calgranulin A)
• S100 calcium-binding protein A8
• S100A8
• Urinary stone protein band A

Background

Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins (1, 2). Mouse S100A8 is an 89 amino acid protein that contains short sequential modules, including an N-terminal alpha -helix, a Ca⁺⁺-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 forms homodimer, and also heterodimerizes with S100A9. In the presence of Ca⁺⁺, the heterodimers form heterotetramers (3, 4). The dimeric S100 A8/A9 complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. Like S100A9, S100A8 is up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (5-7). It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. In addition, S100A8 was shown to activate lung alveolar epithelial cells, osteoclasts, and chondrocytes in TLR4-dependent manner, inducing proinflammatory cytokines and chemokines including, IL-6, IL-8, KC, and MCP-1 (8-10). In osteoarthritic chondrocytes, S100A8 and A9 also promoted expression of matrix metalloproteinases (MMP-1, -3, -9, and -13) potentially affecting to cartilage breakdown (10). The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively (11, 12).

1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
2. Kerkhoff, C. et al. (2012) Exp. Dermatol. 21:822.
3. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
4. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
5. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
6. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
7. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
8. Chakraborty D. et al. (2017) Front Immunol. 8:1493
9. Grevers L.C. Arthritis Rheum. 63:1365.
10. Schelbergen R.F. Arthritis Rheum. 64:1477.
11. Lagasse, E. and I.L. Weissman (1992) Blood 79:1907.
12. Lackmann, M. et al. (1993) J. Immunol. 150:2981.

Publications for S100A8 (9877-S8)(3)

Publications using 9877-S8

• Kitamura, RA;Hummel, D;Ustione, A;Piston, DW;Urano, F; Dual role of neuroplastin in pancreatic ? cells: Regulating insulin secretion and promoting islet inflammation Proceedings of the National Academy of Sciences of the United States of America 2024-08-13 [PMID: 39666939] (Bioassay, Rat)
• Franz, S;Torregrossa, M;Anderegg, U;Ertel, A;Saalbach, A; Dysregulated S100A9 Expression Impairs Matrix Deposition in Chronic Wounds International journal of molecular sciences 2024-09-16 [PMID: 39337466] (Bioassay, Mouse)
• N Maehara, K Taniguchi, A Okuno, H Ando, A Hirota, Z Li, CT Wang, S Arai, T Miyazaki AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke Cell Reports, 2021-09-14;36(11):109693. 2021-09-14 [PMID: 34525359] (Bioassay, Human)

FAQs for S100A8 (9877-S8). (Showing 1 - 2 of 2 FAQs).

1. Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
   • Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
   • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Bioinformatics

• Uniprot:
  • P27005()