Recombinant Mouse Resistin Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse Resistin Protein Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Resistin is used at 0.5 μg/mL, it binds to Biotinylated Recombinant Mouse Leptin with an ED₅₀ of 50-300 ng/mL.
• Source: E. coli-derived mouse Resistin protein
  Ser21-Ser114
• Accession #: Q99P87.1
• N-terminal Sequence: Ser21
• Structure / Form: Monomer and disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Retn
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 10 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Resistin Protein

• Adipose tissue-specific secretory factor
• ADSF
• ADSFMGC126609
• C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1
• C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
• Cysteine-rich secreted protein A12-alpha-like 2
• Cysteine-rich secreted protein FIZZ3
• FIZZ3
• FIZZ3MGC126603
• found in inflammatory zone 3
• HXCP1
• Resistin
• RETN
• RETN1
• RSTNXCP1

Background

Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a member of a family of secreted cysteine-rich peptide hormones that also includes Resistin-like molecules RELM alpha, beta, and gamma. These molecules play important roles in inflammation, glucose metabolism, and insulin resistance (1, 2). Mature mouse Resistin is a 12 kDa protein with an N-terminal alpha -helical domain and a C-terminal beta -sandwich domain that is stabilized by multiple intrachain disulfide bonds (3). Resistin circulates as noncovalent trimers and disulfide-linked hexamers, with the trimeric form showing greater bioactivity (3, 4). Resistin can also form multimers with RELM beta  (4). Mature mouse Resistin shares 56% and 72% amino acid (aa) sequence identity with human and rat Resistin, respectively. It shares 34% - 42% aa sequence identity with mouse RELM alpha , beta , and gamma . In rodents, Resistin is expressed by adipocytes and in the pituitary and arcuate nucleus of the hypothalamus (5 - 9). It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet (5, 6). This is in contrast to human Resistin which is produced by macrophages and monocytes but not by adipocytes (9 - 12). Mouse Resistin induces proinflammatory molecule production in adipocytes and promotes hepatic gluconeogenesis and insulin resistance (5, 7). Human Resistin promotes lipolysis by human and mouse adipocytes, but mouse Resistin does not promote lipolysis by adipocytes of either species (10). Both mouse and human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo (13 - 16).

1. Adeghate, E. (2004) Cell. Mol. Life Sci. 61:2485.
2. Asano, T. et al. (2006) Curr. Diabetes Rev. 2:449.
3. Patel, S.D. et al. (2004) Science 304:1154.
4. Chen, J. et al. (2002) J. Endocrinol. 175:499.
5. Steppan, C.M. et al. (2001) Nature 409:307.
6. Kim, K-H. et al. (2001) J. Biol. Chem. 276:11252.
7. Banerjee, R.R. et al. (2004) Science 303:1195.
8. Rajala, M.W. et al. (2002) Mol. Endocrinol. 16:1920.
9. Morash, B.A. et al. (2002) FEBS Lett. 526:26.
10. Ort, T. et al. (2005) Endocrinology 146:2200.
11. Nagaev, I. et al. (2006) Plos ONE 1:e31.
12. Savage, D.B. et al. (2001) Diabetes 50:2199.
13. Mu, H. et al. (2006) Cardiovasc. Res. 70:146.
14. Robertson, S.A. et al. (2008) Regul. Pept. August 5 epub.
15. Bokarewa, M. et al. (2005) J. Immunol. 174:5789.
16. Burnett, M.S. et al. (2005) Atherosclerosis 182:241.

Publications for Resistin (1069-RN)(2)

Publications using 1069-RN

• Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE, 2015-01-28;10(1):e0117908. 2015-01-28 [PMID: 25629162] (Bioassay, Mouse)
• Blagoev B, Kratchmarova I, Nielsen MM, Fernandez MM, Voldby J, Andersen JS, Kristiansen K, Pandey A, Mann M Inhibition of adipocyte differentiation by resistin-like molecule alpha. Biochemical characterization of its oligomeric nature. J. Biol. Chem., 2002-08-19;277(44):42011-6. 2002-08-19 [PMID: 12189153] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Retn
• Uniprot:
  • Q99P87.1()