Recombinant Mouse Osteopontin/OPN Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Mouse Osteopontin/OPN Protein Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261. When 1 x 105 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED50  for this effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold.
Source
Mouse myeloma cell line, NS0-derived mouse Osteopontin/OPN protein
Leu17-Asn294 (Glu99Gly), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Leu17
Protein/Peptide Type
Recombinant Proteins
Gene
Spp1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
31.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30 kDa and a 65 kDa doublet, reducing conditions
Publications
Read Publications using
441-OP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Osteopontin/OPN Protein

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1-3). Mouse OPN is synthesized as a 294 amino acid (aa) precursor protein with a 16 aa signal peptide and a 278 aa mature protein (3). Mature mouse OPN shares 79% and 64% aa sequence identity with rat and human OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1-4). Depending on tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45-75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6-8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SLAYGLR motif and at sites closer to the C-terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell ad­hesion and migration (1, 2, 4, 6). Its expression is up-regulat­ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).
  1. Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
  2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
  3. Miyazaki, Y. et al. (1990) J. Biol. Chem. 265:14432.
  4. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
  5. Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
  6. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
  7. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
  8. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  9. Takafuji, V. et al. (2007) Oncogene 26:6361.
  10. Kiefer, F.W. et al. (2010) Diabetes 59:935.

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441-OP
Species: Mu
Applications: Bioactivity

Publications for Osteopontin/OPN (441-OP)(26)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Feline, N/A.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(11)
Cell Culture
(1)
ELISA (Standard)
(1)
In Vivo
(10)
Surface Plasmon Resonance
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(21)
Rat
(1)
Feline
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using 441-OP Applications Species
PG Hitscheric, LH Xie, D Del Re, EJ Lee The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models Physiol Rep, 2019;7(13):e14137. 2019 [PMID: 31301118] (Cell Culture, Mouse) Cell Culture Mouse
R Rogall, M Rabenstein, S Vay, A Bach, A Pikhovych, J Baermann, M Hoehn, S Couillard-, GR Fink, M Schroeter, MA Rueger Bioluminescence imaging visualizes osteopontin-induced neurogenesis and neuroblast migration in the mouse brain after stroke Stem Cell Res Ther, 2018;9(1):182. 2018 [PMID: 29973246] (In Vivo, Mouse) In Vivo Mouse
Q Zeng, X Luo, M Han, W Liu, H Li Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity EBioMedicine, 2018;0(0):. 2018 [PMID: 29885866] (In Vivo, Mouse) In Vivo Mouse
RM Natoli, H Yu, MC Meislin, P Abbasnia, P Roper, A Vuchkovska, X Xiao, SR Stock, JJ Callaci Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro J Orthop Surg Res, 2018;13(1):101. 2018 [PMID: 29699560] (Bioassay, Mouse) Bioassay Mouse
MB Alvarez, L Xu, P Childress, KA Maupin, SFG Mohamad, B Chitteti, E Himes, DJ Olivos Iii, YH Cheng, SJ Conway, EF Srour, MA Kacena Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis Stem Cells Dev., 2018;0(0):. 2018 [PMID: 29631496] (Bioassay, Mouse) Bioassay Mouse
G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis Immunol. Cell Biol., 2018;0(0):. 2018 [PMID: 29359350] (In Vivo, Mouse) In Vivo Mouse
M Quattrocel, J Capote, JC Ohiri, JL Warner, AH Vo, JU Earley, M Hadhazy, AR Demonbreun, MJ Spencer, EM McNally Genetic modifiers of muscular dystrophy act on sarcolemmal resealing and recovery from injury PLoS Genet., 2017;13(10):e1007070. 2017 [PMID: 29065150] (In Vivo, Mouse) In Vivo Mouse
W Wang, P Li, W Li, J Jiang, Y Cui, S Li, Z Wang Osteopontin activates mesenchymal stem cells to repair skin wound PLoS ONE, 2017;12(9):e0185346. 2017 [PMID: 28957406] (Bioassay, Mouse) Bioassay Mouse
AM Paul, D Acharya, L Duty, EA Thompson, L Le, DS Stokic, AA Leis, F Bai Osteopontin facilitates West Nile virus neuroinvasion via neutrophil Trojan horse transport Sci Rep, 2017;7(1):4722. 2017 [PMID: 28680095] (Bioassay, Mouse) Bioassay Mouse
CH Chen, YM Sue, CY Cheng, YC Chen, CT Liu, YH Hsu, PA Hwang, NJ Huang, TH Chen Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway Sci Rep, 2017;7(0):40183. 2017 [PMID: 28098144] (Bioassay, Rat) Bioassay Rat
Show All 26 Publications.

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Bioinformatics

Gene Symbol Spp1
Uniprot