Recombinant Mouse NGFR/TNFRSF16 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse NGFR/TNFRSF16 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit beta -NGF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.5-1.5 µg/mL in the presence of 2 ng/mL of rm beta -NGF.
• Source: Mouse myeloma cell line, NS0-derived mouse NGFR/TNFRSF16 protein
  

  Mouse NGF R (Gly20 - Asn243) Accession # Q9Z0W1.1	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q9Z0W1.1
• N-terminal Sequence: Gly20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ngfr
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50.4 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 75-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NGFR/TNFRSF16 Fc Chimera Protein, CF

• CD271 antigen
• CD271
• Gp80-LNGFR
• member 16
• nerve growth factor receptor
• NGF R
• NGF receptor
• NGFR
• p75 ICD
• p75 NTR
• TNFRSF16
• TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
• tumor necrosis factor receptor superfamily member 16

Background

The low affinity nerve growth factor receptor (NGF R), also named p75 neurotrophin receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family and has been designated TNFRSF16. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3 and NT-4 approximately equally with low affinity. The cytoplasmic region of the receptor contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in the amniotic fluid of humans and rats (1 - 3).

1. Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
2. Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
3. Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.

Publications for NGFR/TNFRSF16/p75NTR (1157-NR)(3)

Publications using 1157-NR

• Shepheard S, Chataway T, Schultz D, Rush R, Rogers M The extracellular domain of neurotrophin receptor p75 as a candidate biomarker for amyotrophic lateral sclerosis. PLoS ONE, 2014-01-27;9(1):e87398. 2014-01-27 [PMID: 24475283] (ELISA (Standard), Human)
• Hasebe N, Fujita Y, Ueno M, Yoshimura K, Fujino Y, Yamashita T Soluble beta-amyloid Precursor Protein Alpha binds to p75 neurotrophin receptor to promote neurite outgrowth. PLoS ONE, 2013-12-16;8(12):e82321. 2013-12-16 [PMID: 24358169] (Functional ELISA, Human)
• Vaegter CB, Jansen P, Fjorback AW Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling. Nat. Neurosci., 2010-11-21;14(1):54-61. 2010-11-21 [PMID: 21102451] (Surface Plasmon Resonance)

Bioinformatics

• Gene Symbol: Ngfr
• Uniprot:
  • Q9Z0W1.1()