Recombinant Mouse Lipocalin-2/NGAL Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse Lipocalin-2/NGAL Protein, CF Summary

Details of Functionality
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. >1.0 µM of Fe(DHBA)3 can be bound under the described conditions.
Mouse myeloma cell line, NS0-derived mouse Lipocalin-2/NGAL protein
Gln21-Asn200 with a C-terminal 10 His tag
Accession #
N-terminal Sequence
No results obtained: Gln21 predicted
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
26-27 kDa doublet, reducing conditions
Read Publications using
1857-LC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in MES and NaCl.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (TCN)
  • Ligand Buffer: 0.1 M Tris, pH 8.0
  • Recombinant Mouse Lipocalin‑2/NGAL (rmLipocalin‑2) (Catalog # 1857-LC)
  • Iron III (Fe3+) (Sigma, Catalog # 16596)
  • 2,3,Dihydroxybenzoic Acid (DHBA) (Sigma, Catalog # 126209)
  • F16 Black Maxisorp Plate (Nunc, Catalog #: 475515)
  • Fluorescent Plate Reader (Model: Spectramax Gemini EM by Molecular Devices) or equivalent
  1. Prepare a curve of Fe3+ in deionized water with the following serial dilutions: 640, 320, 160, 80, 40, 20, 10, 5, and 2.5 µM.
  2. Prepare 1 mM DHBA in Ligand Buffer from powder stock.
  3. Combine equal volumes of the Fe3+ curve with 1 mM DHBA. Include a control of 1 mM DHBA and Ligand Buffer.
  4. Incubate at room temperature for 10 minutes.
  5. After incubation, perform 5 fold dilutions to the curve with Assay Buffer.
  6. Dilute rmLipocalin-2 (MW: 21905 Da) to 4 µM in Assay Buffer.
  7. In a plate, load 50 µL of the diluted Fe(DHBA)3 complex curve and 50 µL of 4 µM rmLipocalin-2.
  8. Incubate reaction at room temperature for 30 minutes.
  9. Read at excitation and emission wavelengths of 280 nM and 340 nM, respectively in endpoint mode.
  10. Plot a 4-parameter curve of Fe(DHBA)3 concentration (x-axis) versus RFUs (y-axis), and calculate a BC50 from the curve.
Per Well:
  • Fe(DHBA)3 complex: 0.125, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16, and 32 µM.
  • rmLipocalin-2: 2 µM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Lipocalin-2/NGAL Protein, CF

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin


Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (KD = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

  1. Hraba-Renevey, s. et al. (1989) Oncogene. 4:601. 
  2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293:829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

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Publications for Lipocalin-2/NGAL (1857-LC)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.

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Cell Culture
In Vivo
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 1857-LC Applications Species
L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking Cell Rep, 2019;29(7):2067-2077.e6. 2019 [PMID: 31722218] (Cell Culture, Human) Cell Culture Human
T Ondee, J Gillen, P Visitchana, P Somparn, J Issara-Amp, C Dang Phi, W Chancharoe, D Gurusamy, A Nita-Lazar, A Leelahavan Lipocalin-2 (Lcn-2) Attenuates Polymicrobial Sepsis with LPS Preconditioning (LPS Tolerance) in FcGRIIb Deficient Lupus Mice Cells, 2019;8(9):. 2019 [PMID: 31514375] (In Vivo, Mouse) In Vivo Mouse
A Ishii, G Katsuura, H Imamaki, H Kimura, KP Mori, T Kuwabara, M Kasahara, H Yokoi, K Ohinata, T Kawanishi, J Tsuchida, Y Nakamoto, K Nakao, M Yanagita, M Mukoyama, K Mori Obesity-promoting and anti-thermogenic effects of neutrophil gelatinase-associated lipocalin in mice Sci Rep, 2017;7(1):15501. 2017 [PMID: 29138470] (In Vivo, Mouse) In Vivo Mouse
AC Ferreira, T Santos, B Sampaio-Ma, A Novais, SD Mesquita, P Ludovico, L Bernardino, M Correia-Ne, N Sousa, JA Palha, JC Sousa, F Marques Lipocalin-2 regulates adult neurogenesis and contextual discriminative behaviours Mol. Psychiatry, 2017;0(0):. 2017 [PMID: 28485407] (Bioassay, Mouse) Bioassay Mouse
The adipokine lipocalin-2 in the context of the osteoarthritic osteochondral junction Sci Rep, 2016;6(0):29243. 2016 [PMID: 27385438] (Bioassay, Mouse) Bioassay Mouse
Rucci N, Capulli M, Piperni S, Cappariello A, Lau P, Frings-Meuthen P, Heer M, Teti A Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis. J Bone Miner Res, 2015;30(2):357-68. 2015 [PMID: 25112732] (Bioassay, Mouse) Bioassay Mouse
Maung R, Hoefer M, Sanchez A, Sejbuk N, Medders K, Desai M, Catalan I, Dowling C, de Rozieres C, Garden G, Russo R, Roberts A, Williams R, Kaul M CCR5 knockout prevents neuronal injury and behavioral impairment induced in a transgenic mouse model by a CXCR4-using HIV-1 glycoprotein 120. J Immunol, 2014;193(4):1895-910. 2014 [PMID: 25031461] (Bioassay, Mouse) Bioassay Mouse
Miao Q, Ku A, Nishino Y, Howard J, Rao A, Shaver T, Garcia G, Le D, Karlin K, Westbrook T, Poli V, Nguyen H Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2. Nat Commun, 2014;5(0):4088. 2014 [PMID: 24909826] (In Vivo, Mouse) In Vivo Mouse
Jeon, Sangmin, Jha, Mithiles, Ock, Jiyeon, Seo, Jungwan, Jin, Myungwon, Cho, Heejung, Lee, Won-Ha, Suk, Kyoungho Role of lipocalin-2-chemokine axis in the development of neuropathic pain following peripheral nerve injury. J Biol Chem, 2013;288(33):24116-27. 2013 [PMID: 23836894] (In Vivo, Mouse) In Vivo Mouse
Shashidharamurthy R, Machiah D, Aitken J, Putty K, Srinivasan G, Chassaing B, Parkos C, Selvaraj P, Vijay-Kumar M Differential role of lipocalin 2 during immune complex-mediated acute and chronic inflammation in mice. Arthritis Rheum, 2013;65(4):1064-73. 2013 [PMID: 23280250] (In Vivo, Mouse) In Vivo Mouse
Show All 12 Publications.

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Gene Symbol Lcn2