Recombinant Mouse Lipocalin-2/NGAL Protein, CF


There are currently no images for Lipocalin-2/NGAL (1857-LC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

Order Details

Recombinant Mouse Lipocalin-2/NGAL Protein, CF Summary

Details of Functionality
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. >1.0 µM of Fe(DHBA)3 can be bound under the described conditions.
Mouse myeloma cell line, NS0-derived mouse Lipocalin-2/NGAL protein
Gln21-Asn200 with a C-terminal 10 His tag
Accession #
N-terminal Sequence
No results obtained: Gln21 predicted
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
26-27 kDa doublet, reducing conditions
Read Publications using
1857-LC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in MES and NaCl.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (TCN)
  • Ligand Buffer: 0.1 M Tris, pH 8.0
  • Recombinant Mouse Lipocalin‑2/NGAL (rmLipocalin‑2) (Catalog # 1857-LC)
  • Iron III (Fe3+) (Sigma, Catalog # 16596)
  • 2,3,Dihydroxybenzoic Acid (DHBA) (Sigma, Catalog # 126209)
  • F16 Black Maxisorp Plate (Nunc, Catalog #: 475515)
  • Fluorescent Plate Reader (Model: Spectramax Gemini EM by Molecular Devices) or equivalent
  1. Prepare a curve of Fe3+ in deionized water with the following serial dilutions: 640, 320, 160, 80, 40, 20, 10, 5, and 2.5 µM.
  2. Prepare 1 mM DHBA in Ligand Buffer from powder stock.
  3. Combine equal volumes of the Fe3+ curve with 1 mM DHBA. Include a control of 1 mM DHBA and Ligand Buffer.
  4. Incubate at room temperature for 10 minutes.
  5. After incubation, perform 5 fold dilutions to the curve with Assay Buffer.
  6. Dilute rmLipocalin-2 (MW: 21905 Da) to 4 µM in Assay Buffer.
  7. In a plate, load 50 µL of the diluted Fe(DHBA)3 complex curve and 50 µL of 4 µM rmLipocalin-2.
  8. Incubate reaction at room temperature for 30 minutes.
  9. Read at excitation and emission wavelengths of 280 nM and 340 nM, respectively in endpoint mode.
  10. Plot a 4-parameter curve of Fe(DHBA)3 concentration (x-axis) versus RFUs (y-axis), and calculate a BC50 from the curve.
Per Well:
  • Fe(DHBA)3 complex: 0.125, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16, and 32 µM.
  • rmLipocalin-2: 2 µM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Lipocalin-2/NGAL Protein, CF

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin


Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (KD = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

  1. Hraba-Renevey, s. et al. (1989) Oncogene. 4:601. 
  2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293:829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

Publications for Lipocalin-2/NGAL (1857-LC)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.

Filter By Application
Cell Culture
In Vivo
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using 1857-LC Applications Species
Su, H;Guo, H;Qiu, X;Lin, TY;Qin, C;Celio, G;Yong, P;Senders, M;Han, X;Bernlohr, DA;Chen, X; Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice Nature communications 2023-10-23 [PMID: 37872178] (Bioassay, Mouse) Bioassay Mouse
Liu, Y;Smith, MR;Wang, Y;D'Agostino, R;Ruiz, J;Lycan, T;Kucera, GL;Miller, LD;Li, W;Chan, MD;Farris, M;Su, J;Song, Q;Zhao, D;Chandrasekaran, A;Xing, F; c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities Cancers 2023-05-05 [PMID: 37174093]
JR Sheldon, LE Himmel, DE Kunkle, AJ Monteith, KN Maloney, EP Skaar Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection PloS Pathogens, 2022-09-02;18(9):e1010809. 2022-09-02 [PMID: 36054235] (Bioassay, Mouse) Bioassay Mouse
X Xiang, X Tang, Y Yu, S Xie, L Liu, M Chen, R Zhang, X Kang, Y Zheng, G Yang, S Gan, S Zhu Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia Journal of Neuroinflammation, 2022-04-12;19(1):92. 2022-04-12 [PMID: 35413913] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
C Huisman, MA Norgard, PR Levasseur, SM Krasnow, MGP van der Wi, B Olson, DL Marks Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing Molecular Metabolism, 2022-01-11;0(0):101441. 2022-01-11 [PMID: 35031523] (In Vivo, Mouse) In Vivo Mouse
X Wang, X Li, X Zuo, Z Liang, T Ding, K Li, Y Ma, P Li, Z Zhu, C Ju, Z Zhang, Z Song, H Quan, J Zhang, X Hu, Z Wang Photobiomodulation inhibits the activation of neurotoxic microglia and astrocytes by inhibiting Lcn2/JAK2-STAT3 crosstalk after spinal cord injury in male rats Journal of Neuroinflammation, 2021-11-05;18(1):256. 2021-11-05 [PMID: 34740378] (Bioassay, Rat) Bioassay Rat
YH Chen, SY Xie, CW Chen, DY Lu Electroacupuncture improves repeated social defeat stress-elicited social avoidance and anxiety-like behaviors by reducing Lipocalin-2 in the hippocampus Molecular Brain, 2021-09-26;14(1):150. 2021-09-26 [PMID: 34565419] (In Vivo, Mouse) In Vivo Mouse
B Olson, X Zhu, MA Norgard, P Diba, PR Levasseur, AC Buenafe, C Huisman, KG Burfeind, KA Michaelis, G Kong, T Braun, DL Marks Chronic cerebral lipocalin 2 exposure elicits hippocampal neuronal dysfunction and cognitive impairment Brain, Behavior, and Immunity, 2021-07-08;0(0):. 2021-07-08 [PMID: 34245812] (In Vivo, Mouse) In Vivo Mouse
B Olson, X Zhu, MA Norgard, PR Levasseur, JT Butler, A Buenafe, KG Burfeind, KA Michaelis, KR Pelz, H Mendez, J Edwards, SM Krasnow, AJ Grossberg, DL Marks Lipocalin 2 mediates appetite suppression during pancreatic cancer cachexia Nature Communications, 2021-04-06;12(1):2057. 2021-04-06 [PMID: 33824339] (Bioassay, Mouse) Bioassay Mouse
J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation Cell Rep, 2020-06-30;31(13):107820. 2020-06-30 [PMID: 32610123] (Bioassay, Mouse) Bioassay Mouse
Show All 23 Publications.

Reviews for Lipocalin-2/NGAL (1857-LC) (0)

There are no reviews for Lipocalin-2/NGAL (1857-LC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Lipocalin-2/NGAL (1857-LC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Lipocalin-2/NGAL Products

Blogs on Lipocalin-2/NGAL

There are no specific blogs for Lipocalin-2/NGAL, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse Lipocalin-2/NGAL Protein, CF and receive a gift card or discount.


Gene Symbol Lcn2