Recombinant Mouse Integrin alpha V beta 3 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Integrin alpha V beta 3 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Vitronectin (Catalog # 2308-VN) at 1 µg/mL can bind Recombinant Mouse Integrin alpha V beta 3 with an apparent KD <5 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha V beta 3 protein
Mouse Integrin alpha V
(Phe31-Val988)
Accession # P43406
His-Pro GGGSGGGS Acidic Tail 6-His tag
Mouse Integrin beta 3
(Glu26-Asp717)
Accession # O54890
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe31 (Integrin alpha V) & Glu26 (Integrin beta 3)

Structure / Form
Noncovalently-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
115 kDa (Integrin alpha V) & 84.5 kDa (Integrin beta 3).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
135-145 kDa & 100-115 kDa, reducing conditions
Publications
Read Publication using
7889-AV in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha V beta 3 Protein, CF

  • antigen identified by monoclonal L230
  • CD51 antigen
  • CD51
  • Integrin alpha V beta 3
  • integrin alpha-V
  • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
  • MSK8
  • Vitronectin receptor subunit alpha
  • VNRADKFZp686A08142

Background

Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1‑3). The non‑covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N‑terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine‑rich I‑EGF folds. The alpha V subunit domains termed thigh, calf‑1 and calf‑2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). The 958 aa mouse alpha V ECD shares 92‑95% aa sequence identity with human, rat and bovine  alpha V while the 692 aa mouse beta 3 ECD shares 95% aa identity with rat and 89‑91% with human, canine, equine, bovine and porcine beta 3. alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (5, 6). M‑CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (5, 6). alpha V beta 3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF‑1 to promote cancer cell proliferation and invasiveness (7, 8). Also, cell entry of several viruses is mediated by alpha V beta 3 (4, 9).

  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  6. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  7. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
  8. Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106.
  9. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.

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Publications for Integrin alpha V beta 3 (7889-AV)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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