Recombinant Mouse IL-4 Protein

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1 μg/lane of Recombinant Mouse IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.
Recombinant Mouse IL-4 (Catalog # 404‑ML) stimulates cell proliferation of the HT‑2 mouse T cell line. The ED50 for this effect is 0.3-1.5 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications BA

Order Details

Recombinant Mouse IL-4 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using HT‑2 mouse T cells. The ED50 for this effect is 0.3‑1.5 ng/mL.
Source
E. coli-derived
His23-Ser140, with an N-terminal Met
Accession #
N-terminal Sequence
His23
Protein/Peptide Type
Recombinant Proteins
Gene
Il4
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
404-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 aa signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and
-21). The type II receptor on nonhematopoietic cells consists of IL‑4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL‑13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 ‑ 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

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Publications for (205)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, Cell Culture Substrate, ELISA Standard, In Vivo, Luminex standard, Media Supplement - Cell Prep.


Filter By Application
Bioassay
(187)
Cell Culture Substrate
(1)
ELISA Standard
(4)
In Vivo
(8)
Luminex standard
(1)
Media Supplement - Cell Prep
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(197)
N/A
(4)
All Species
Showing Publications 1 - 10 of 205. Show All 205 Publications.
Publications using 404-ML Applications Species
S Mroczek, J Chlebowska, TM Kuli?ski, O Gewartowsk, J Gruchota, D Cysewski, V Liudkovska, E Borsuk, D Nowis, A Dziembowsk The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma Nat Commun, 2017;8(1):619. 2017 [PMID: 28931820] (Bioassay, Mouse) Bioassay Mouse
T Rivera Var, Z Cai, Y Shen, M Dosset, I Benoit-Liz, T Martin, A Roussey, RA Flavell, F Ghiringhel, L Apetoh Selective degradation of PU.1 during autophagy represses the differentiation and antitumour activity of TH9 cells Nat Commun, 2017;8(1):559. 2017 [PMID: 28916785] (Bioassay, Mouse) Bioassay Mouse
DK Chiu, AP Tse, IM Xu, J Di Cui, RK Lai, LL Li, HY Koh, FH Tsang, LL Wei, CM Wong, IO Ng, CC Wong Hypoxia inducible factor HIF-1 promotes myeloid-derived suppressor cells accumulation through ENTPD2/CD39L1 in hepatocellular carcinoma Nat Commun, 2017;8(1):517. 2017 [PMID: 28894087] (Bioassay, Mouse) Bioassay Mouse
N Akbar, JE Digby, TJ Cahill, AN Tavare, AL Corbin, S Saluja, S Dawkins, L Edgar, N Rawlings, K Ziberna, E McNeill,, E Johnson, AA Aljabali, RA Dragovic, M Rohling, TG Belgard, IA Udalova, DR Greaves, KM Channon, PR Riley, DC Anthony, RP Choudhury Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction JCI Insight, 2017;2(17):. 2017 [PMID: 28878126] (Bioassay, Mouse) Bioassay Mouse
SMP Vadevoo, JE Kim, GR Gunassekar, HK Jung, L Chi, DE Kim, SH Lee, SH Im, B Lee Interleukin 4 receptor-targeted pro-apoptotic peptide blocks tumor growth and metastasis by enhancing anti-tumor immunity Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28878029] (Bioassay, Mouse) Bioassay Mouse
JP Antonios, H Soto, RG Everson, DL Moughon, AC Wang, J Orpilla, C Radu, BM Ellingson, JT Lee, T Cloughesy, ME Phelps, J Czernin, LM Liau, RM Prins Detection of immune responses after immunotherapy in glioblastoma using PET and MRI Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28874539] (Bioassay, Mouse) Bioassay Mouse
MH Ross, AK Esser, GC Fox, AH Schmieder, X Yang, G Hu, D Pan, X Su, Y Xu, DV Novack, T Walsh, GA Colditz, GH Lukaszewic, E Cordell, JS Novack, JAJ Fitzpatric, DL Waning, KS Mohammad, TA Guise, GM Lanza, KN Weilbaeche Bone-induced expression of integrin ?3 enables targeted nanotherapy of breast cancer metastases Cancer Res., 2017;0(0):. 2017 [PMID: 28855208] (Bioassay, Mouse) Bioassay Mouse
KA Read, MD Powell, CE Baker, BK Sreekumar, VM Ringel-Sca, H Bachus, RE Martin, ID Cooley, IC Allen, A Ballestero, KJ Oestreich Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4(+) Th Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28848064] (Bioassay, Mouse) Bioassay Mouse
T Hosokawa, T Kimura, S Nada, T Okuno, D Ito, S Kang, S Nojima, K Yamashita, T Nakatani, Y Hayama, Y Kato, Y Kinehara, M Nishide, N Mikami, S Koyama, H Takamatsu, D Okuzaki, N Ohkura, S Sakaguchi, M Okada, A Kumanogoh Lamtor1 Is Critically Required for CD4(+) T Cell Proliferation and Regulatory T Cell Suppressive Function J. Immunol., 2017;0(0):. 2017 [PMID: 28768723] (Bioassay, Mouse) Bioassay Mouse
RD Vicetti Mi, NE Quispe Cal, D Dixon, RA Foster, A Gambotto, SD Pavelko, L Hall-Stood, TL Cherpes IL-4-secreting eosinophils promote endometrial stromal cell proliferation and prevent Chlamydia-induced upper genital tract damage Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28765368] (Bioassay, Mouse) Bioassay Mouse
Show All 205 Publications.

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Bioinformatics

Gene Symbol Il4
Entrez
Uniprot