Recombinant Mouse IL-4 Protein

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1 μg/lane of Recombinant Mouse IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.
Recombinant Mouse IL-4 (Catalog # 404‑ML) stimulates cell proliferation of the HT‑2 mouse T cell line. The ED50 for this effect is 0.3-1.5 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse IL-4 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using HT-2 mouse T cells. The ED 50 for this effect is 0.3-1.5 ng/mL.
Source
E. coli-derived mouse IL-4 protein His23-Ser140, with an N-terminal Met
Accession #
N-terminal Sequence
His23
Protein/Peptide Type
Recombinant Proteins
Gene
Il4
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
404-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 aa signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4 + T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4 + T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 - 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Lee, F. et al. (1986) Proc. Natl. Acad. Sci. 83:2061.
  4. Noma, Y. et al. (1986) Nature 319:640.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  12. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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Publications for IL-4 (404-ML)(233)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, Cell Culture Substrate, ELISA Standard, In Vivo, Luminex standard, Media Supplement - Cell Prep.


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Bioassay
(213)
Cell Culture Substrate
(1)
ELISA Standard
(6)
In Vivo
(8)
Luminex standard
(1)
Media Supplement - Cell Prep
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(225)
N/A
(4)
All Species
Showing Publications 1 - 10 of 233. Show All 233 Publications.
Publications using 404-ML Applications Species
BG Barwick, CD Scharer, RJ Martinez, MJ Price, AN Wein, RR Haines, APR Bally, JE Kohlmeier, JM Boss B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation Nat Commun, 2018;9(1):1900. 2018 [PMID: 29765016] (Bioassay, Mouse) Bioassay Mouse
CD Scharer, BG Barwick, M Guo, APR Bally, JM Boss Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs Nat Commun, 2018;9(1):1698. 2018 [PMID: 29703886] (Bioassay, Mouse) Bioassay Mouse
S Nowsheen, K Aziz, A Aziz, M Deng, B Qin, K Luo, KB Jeganathan, H Zhang, T Liu, J Yu, Y Deng, J Yuan, W Ding, JM van Deurse, Z Lou L3MBTL2 orchestrates ubiquitin signalling by dictating the sequential recruitment of RNF8 and RNF168 after DNA damage Nat. Cell Biol., 2018;20(4):455-464. 2018 [PMID: 29581593] (Bioassay, Mouse) Bioassay Mouse
Y Kasagi, PM Chandramou, KA Whelan, K Tanaka, V Giroux, M Sharma, J Wang, AJ Benitez, M DeMarshall, JW Tobias, KE Hamilton, GW Falk, JM Spergel, AJ Klein-Szan, AK Rustgi, AB Muir, H Nakagawa The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial�Changes Cell Mol Gastroenterol Hepatol, 2018;5(3):333-352. 2018 [PMID: 29552622] (Bioassay, Mouse) Bioassay Mouse
C Li, T Irrazabal, CC So, M Berru, L Du, E Lam, AK Ling, JL Gommerman, Q Pan-Hammar, A Martin The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining Nat Commun, 2018;9(1):1006. 2018 [PMID: 29520062] (Bioassay, Mouse) Bioassay Mouse
B Sun, L Zhu, Y Tao, HX Sun, Y Li, P Wang, Y Hou, Y Zhao, X Zhang, L Zhang, N Na, Y Zhao Characterization and allergic role of IL-33-induced neutrophil polarization Cell. Mol. Immunol., 2018;0(0):. 2018 [PMID: 29503441] (Bioassay, Mouse) Bioassay Mouse
N Andreas, M Riemann, CN Castro, M Groth, I Koliesnik, C Engelmann, T Sparwasser, T Kamradt, R Haenold, F Weih A new RelB-dependent CD117+CD172a+murine dendritic cell subset preferentially induces Th2 differentiation and supports airway hyperresponses in vivo Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29485182] (Bioassay, Mouse) Bioassay Mouse
K Lee, N Park, H Jung, YA Rim, Y Nam, J Lee, SH Park, JH Ju Mesenchymal stem cells ameliorate experimental arthritis via expression of interleukin-1 receptor antagonist PLoS ONE, 2018;13(2):e0193086. 2018 [PMID: 29481574] (Bioassay, Mouse) Bioassay Mouse
D Szpak, L Izem, D Verbovetsk, DA Soloviev, VP Yakubenko, E Pluskota ?M?2Is Antiatherogenic in Female but Not Male Mice J. Immunol., 2018;0(0):. 2018 [PMID: 29459405] (Bioassay, Mouse) Bioassay Mouse
MJ Tol, MJC van der Li, TL Gabriel, JJ Hagen, S Scheij, T Veenendaal, J Klumperman, WE Donker-Koo, AJ Verhoeven, H Overkleeft, JM Aerts, CA Argmann, M van Eijk HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution Autophagy, 2018;0(0):1-13. 2018 [PMID: 29455584] (Bioassay, Mouse) Bioassay Mouse
Show All 233 Publications.

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Bioinformatics

Gene Symbol Il4
Entrez
Uniprot