Recombinant Mouse IL-4 Protein

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1 μg/lane of Recombinant Mouse IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.
Recombinant Mouse IL-4 (Catalog # 404‑ML) stimulates cell proliferation of the HT‑2 mouse T cell line. The ED50 for this effect is 0.3-1.5 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse IL-4 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using HT‑2 mouse T cells. The ED50 for this effect is 0.3‑1.5 ng/mL.
Source
E. coli-derived mouse IL-4 protein
His23-Ser140, with an N-terminal Met
Accession #
N-terminal Sequence
His23
Protein/Peptide Type
Recombinant Proteins
Gene
Il4
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
404-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 aa signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and
-21). The type II receptor on nonhematopoietic cells consists of IL‑4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL‑13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 ‑ 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Lee, F. et al. (1986) Proc. Natl. Acad. Sci. 83:2061.
  4. Noma, Y. et al. (1986) Nature 319:640.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  12. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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Publications for IL-4 (404-ML)(248)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, Cell Culture Substrate, ELISA Standard, In Vivo, Luminex standard, Media Supplement - Cell Prep.


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Bioassay
(227)
Cell Culture Substrate
(1)
ELISA Standard
(6)
In Vivo
(8)
Luminex standard
(1)
Media Supplement - Cell Prep
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(239)
N/A
(4)
All Species
Showing Publications 1 - 10 of 248. Show All 248 Publications.
Publications using 404-ML Applications Species
R Sugamata, A Donko, Y Murakami, HE Boudreau, CF Qi, J Kwon, TL Leto Duox1 Regulates Primary B Cell Function under the Influence of IL-4 through BCR-Mediated Generation of Hydrogen Peroxide J. Immunol., 2018;0(0):. 2018 [PMID: 30559322] (Bioassay, Mouse) Bioassay Mouse
L Simula, I Pacella, A Colamatteo, C Procaccini, V Cancila, M Bordi, C Tregnago, M Corrado, M Pigazzi, V Barnaba, C Tripodo, G Matarese, S Piconese, S Campello Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming Cell Rep, 2018;25(11):3059-3073.e10. 2018 [PMID: 30540939] (Bioassay, Mouse) Bioassay Mouse
H Chiu, LV Jackson, KI Oh, A Mai, ZA Ronai, D Ruggero, DA Fruman The mTORC1/4E-BP/eIF4E Axis Promotes Antibody Class Switching in B Lymphocytes J. Immunol., 2018;0(0):. 2018 [PMID: 30530594] (Bioassay, Mouse) Bioassay Mouse
X Liu, P Zhang, Y Zhang, Z Wang, S Xu, Y Li, W Huai, Q Zhou, X Chen, X Chen, N Li, P Wang, Y Li, X Cao Glycolipid iGb3 feedback amplifies innate immune responses via CD1d reverse signaling Cell Res., 2018;0(0):. 2018 [PMID: 30514903] (Bioassay, Mouse) Bioassay Mouse
W Sun, N Meednu, A Rosenberg, J Rangel-Mor, V Wang, J Glanzman, T Owen, X Zhou, H Zhang, BF Boyce, JH Anolik, L Xing B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation Nat Commun, 2018;9(1):5127. 2018 [PMID: 30510188] (Bioassay, Mouse) Bioassay Mouse
C Skon-Hegg, J Zhang, X Wu, M Sagolla, N Ota, A Wuster, J Tom, E Doran, N Ramamoorth, P Caplazi, J Monroe, WP Lee, TW Behrens LACC1 Regulates TNF and IL-17 in Mouse Models of Arthritis and Inflammation J. Immunol., 2018;0(0):. 2018 [PMID: 30510070] (Bioassay, Mouse) Bioassay Mouse
C Farruggia, MB Kim, M Bae, Y Lee, TX Pham, Y Yang, MJ Han, YK Park, JY Lee Astaxanthin exerts anti-inflammatory and antioxidant effects in macrophages in NRF2-dependent and independent manners J. Nutr. Biochem., 2018;62(0):202-209. 2018 [PMID: 30308382] (Bioassay, Mouse) Bioassay Mouse
M Becerra-Dí, AB Strickland, A Keselman, NM Heller Androgen and Androgen Receptor as Enhancers of M2 Macrophage Polarization in Allergic Lung Inflammation J. Immunol., 2018;0(0):. 2018 [PMID: 30305328]
Y Wang, S Lv, Q Wang, C Wang, M Zhu, Z Ma, W Zhao Mechanisms underlying immune tolerance caused by recombinant Echinococcus granulosus antigens Eg mMDH and Eg10 in dendritic cells PLoS ONE, 2018;13(9):e0204868. 2018 [PMID: 30261049] (Bioassay, Mouse) Bioassay Mouse
S Trezise, A Karnowski, PL Fedele, S Mithraprab, Y Liao, K D'Costa, AJ Kueh, MP Hardy, CM Owczarek, MJ Herold, A Spencer, W Shi, SN Willis, SL Nutt, LM Corcoran Mining the Plasma Cell Transcriptome for Novel Cell Surface Proteins Int J Mol Sci, 2018;19(8):. 2018 [PMID: 30042348] (Bioassay, Mouse) Bioassay Mouse
Show All 248 Publications.

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Bioinformatics

Gene Symbol Il4
Uniprot