Recombinant Mouse IL-31 Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

Order Details

Recombinant Mouse IL-31 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑31 RA Fc Chimera (Catalog # 1253-IL) is immobilized at 5 µg/mL (100 µL/well), Recombinant Mouse IL‑31 binds with an ED50 of 0.9-5.4 μg/mL.
E. coli-derived mouse IL-31 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
Read Publication using
3028-ML in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetic Acid and NaCl.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in 10 mM Acetic Acid.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-31 Protein, CF

  • IL31
  • IL-31
  • IL-31interleukin-31
  • interleukin 31


Mouse Interleukin-31 (IL-31) is likely a secreted, 24‑33 kDa short-chain member of the alpha -helical IL-6 family of cytokines (1, 2). The mouse IL‑31 cDNA encodes a 163 amino acid (aa) precursor that contains a 23 aa signal peptide and a 140 aa mature protein (3, 4). The mature region shows four alpha ‑helices which would be expected to generate a typical up-up-down-down topology. There are three potential sites for N‑linked glycosylation. Mature mouse IL-31 shares 29% and 63% aa sequence identity with human and rat IL-31, respectively. Neither mouse nor human IL-31 are active on their counterparts receptors (1). IL-31 is associated with activated T cells and is preferentially expressed by Th2 rather than Th1 cells (1). IL-31 signals via a heterodimeric receptor complex composed of a newly identified, 120 kDa, gp130-related molecule termed IL-31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ) (4‑8). In the complex, IL‑31 directly binds to IL‑31 RA, not OSM R (4, 5). IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and MAP kinase pathway. Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable (4‑7). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFN-gamma on monocytes (1, 3). Other cells known to be responsive to IL-31 include bronchial epithelium, type II alveolar cells, goblet cells, and likely hematopoietic progenitor cells (9‑11). Functionally, it has been shown that IL-31 may contribute to clinical pruritis (itching) associated with nonatopic dermatitis (1, 3, 12). This may be a consequence of IL-31’s ability to induce keratinocyte secretion of multiple cytokines, including TARC, GRO‑ alpha, MIP‑3 beta and I-309 (1). In addition, IL-31 promotes proliferation of high density cell populations such as myeloid progenitor cells, but conversely suppresses proliferation of lung epithelial cells (1). Finally, IL-31 may actually have a modulating effect on T cell subsets, influencing the ratio between Th1 and Th2 cells (1).

  1. Zhang, Q. et al. (2008) Cytokine Growth Factor Rev. 19:347.
  2. Venereau, E. et al. (2009) J. Biol. Chem. 285:14955.
  3. Dillon SR, et al. (2004) Nat. Immunol. 5:752.
  4. Diveu C, et al. (2004) Eur. Cytokine Netw. 15:291.
  5. Dreuw A, et al. (2004) J. Biol. Chem. 279:36112.
  6. Dieu C, et al. (2003) J. Biol. Chem. 278:49850.
  7. Ghilardi N, et al. (2002) J. Biol. Chem 277:16831.
  8. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
  9. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
  10. Perrigoue, J.G. (2009) J. Immunol. 182:6088.
  11. Broxmeyer, H.E. et al. (2007) Exp. Hematol. 35 (Suppl 1):78.
  12. Takaoka, A. et al. (2005) Eur. J. Pharmacol. 516:180.

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Publications for IL-31 (3028-ML)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: In Vivo.

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Gene Symbol Il31