NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-31 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Human IL-31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2824) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2824-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 2824-IL-010/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3028-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB28241-SP | |||
1253-IL) is immobilized at 5 µg/mL, Recombinant Mouse IL-31 (Catalog # 3028-ML) binds with an ED50 of 0.9-5.4 µg/mL." alt="When Recombinant Mouse IL-31 RA Fc Chimera (1253-IL) is immobilized at 5 µg/mL, Recombinant Mouse IL-31 (Catalog # 3028-ML) binds with an ED50 of 0.9-5.4 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77256-0.025mg | |||
![SDS-Page: Recombinant Canine IL-31 Protein [NBP2-59591] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Canine-IL-31-Protein-SDS-Page-NBP2-59591-img0001.jpg "SDS-Page: Recombinant Canine IL-31 Protein [NBP2-59591] - 15% SDS-PAGE (3ug)")
![N/A IL-31 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL31_DY2824_ELISA_1892.jpg "N/A IL-31 [Biotin]")
2824-IL) was serially diluted and captured by Goat Anti-Human IL‑31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2824) coated on a Clear Polystyrene Microplate (Catalog # 
2824-IL) was serially diluted and captured by Goat Anti-Human IL‑31 Antigen Affinity-purified Polyclonal Antibody (Catalog # ![Immunocytochemistry/Immunofluorescence: IL-31 Antibody (8-C3) [NBP2-80396] - Staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-31-Antibody-8-C3-Immunocytochemistry-Immunofluorescence-NBP2-80396-img0003.jpg "Immunocytochemistry/Immunofluorescence: IL-31 Antibody (8-C3) [NBP2-80396] - Staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: IL-31 Antibody (8-C3) [NBP2-80396] - Analysis in human spleen tissue using IL-31 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IL-31-Antibody-8-C3-Immunohistochemistry-Paraffin-NBP2-80396-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IL-31 Antibody (8-C3) [NBP2-80396] - Staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-31-Antibody-8-C3-Immunocytochemistry-Immunofluorescence-NBP2-80396-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IL-31 Antibody (8-C3) [NBP2-80396] - Staining IL-31 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-31-Antibody-8-C3-Immunocytochemistry-Immunofluorescence-NBP2-80396-img0002.jpg)
![Immunohistochemistry-Paraffin: IL-31 Antibody (8-C3) [NBP2-80396] - Analysis in human tonsil tissue using IL-31 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IL-31-Antibody-8-C3-Immunohistochemistry-Paraffin-NBP2-80396-img0004.jpg)
![SDS-Page: Recombinant Canine IL-31 Protein [NBP2-77493] - 3ug under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Canine-IL-31-Protein-SDS-Page-NBP2-77493-img0001.jpg "SDS-Page: Recombinant Canine IL-31 Protein [NBP2-77493] - 3ug under reducing condition and visualized by coomassie blue stain")
![Western Blot: IL-31 Antibody - BSA Free [NBP1-03044] - Western blot of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 ug/ml.](http://images.novusbio.com/fullsize/IL-31-Antibody---BSA-Free-Western-Blot-NBP1-03044-img0004.jpg "Western Blot: IL-31 Antibody - BSA Free [NBP1-03044] - Western blot of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 ug/ml.")
![Immunohistochemistry-Paraffin: IL-31 Antibody - BSA Free [NBP1-03044] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/IL-31-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-03044-img0002.jpg)
![Immunofluorescence: IL-31 Antibody - BSA Free [NBP1-03044] - Immunofluorescence of IL-31 in Human Skeletal Muscle cells with IL-31 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/IL-31-Antibody---BSA-Free-Immunofluorescence-NBP1-03044-img0003.jpg)
![Western Blot: IL-31 Antibody [NBP1-77256] - RAW264.7 cell lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 ug/ml.](http://images.novusbio.com/fullsize/IL-31-Antibody-Western-Blot-NBP1-77256-img0002.jpg "Western Blot: IL-31 Antibody [NBP1-77256] - RAW264.7 cell lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 ug/ml.")
![Immunohistochemistry: IL-31 Antibody - BSA Free [NBP1-77256] - Immunohistochemistry of IL-31 in rat skeletal muscle tissue with IL-31 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77256_rabbit-polyclonal-il-31-antibody-2711202420141975.jpg)
