Recombinant Mouse IL-23 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-23 Protein Summary

Details of Functionality
Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.05-0.25 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse IL-23 protein
Mouse IL-23 p40
(Met23-Ser335)
Accession # P43432
IGSGSSRGGSGSGGSGGGGSK Mouse IL-23 p19
(Leu20-Ala196)
Accession # Q9EQ14
N-terminus C-terminus
Accession #
N-terminal Sequence
Met23
Protein/Peptide Type
Recombinant Proteins
Gene
Il23a
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
66 kDa, reducing conditions
Publications
Read Publications using
1887-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-23 Protein

  • IL-23 p19/IL-12 p40
  • IL23
  • IL-23
  • IL23A
  • IL-23A
  • IL-23-A
  • IL-23p19
  • IL-23p19/IL-12p40
  • IL23P19P19
  • interleukin 23 p19 subunit
  • interleukin 23, alpha subunit p19
  • interleukin-23 subunit alpha
  • Interleukin-23 subunit p19
  • JKA3 induced upon T-cell activation
  • MGC79388
  • SGRF
  • SGRFIL-23 subunit alpha

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.
  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

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Publications for IL-23A/IL-23 P19 (1887-ML)(118)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture Substrate, In Vivo, cell culture.


Filter By Application
Bioassay
(99)
Cell Culture Substrate
(1)
In Vivo
(14)
cell culture
(1)
All Applications
Filter By Species
Mouse
(115)
All Species
Showing Publications 1 - 10 of 118. Show All 118 Publications.
Publications using 1887-ML Applications Species
L Bouchareyc, EM Grössinger, M Kang, IE Adamopoulo ??TCR regulates production of interleukin-27 by neutrophils and attenuates inflammatory arthritis Sci Rep, 2018;8(1):7590. 2018 [PMID: 29765156] (Bioassay, Mouse) Bioassay Mouse
VL Ngo, H Abo, E Maxim, A Harusato, D Geem, O Medina-Con, D Merlin, AT Gewirtz, A Nusrat, TL Denning A cytokine network involving IL-36?, IL-23, and IL-22 promotes antimicrobial defense and recovery from intestinal barrier damage Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29760082] (Bioassay, Mouse) Bioassay Mouse
R Wu, J Zeng, J Yuan, X Deng, Y Huang, L Chen, P Zhang, H Feng, Z Liu, Z Wang, X Gao, H Wu, H Wang, Y Su, M Zhao, Q Lu MicroRNA-210 overexpression promotes psoriasis-like inflammation by inducing Th1 and Th17 cell differentiation J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29757188] (In Vivo, Mouse) In Vivo Mouse
Y Jiang, Y Liu, H Lu, SC Sun, W Jin, X Wang, C Dong Epigenetic activation during T helper 17 cell differentiation is mediated by Tripartite motif containing 28 Nat Commun, 2018;9(1):1424. 2018 [PMID: 29651155] (Bioassay, Mouse) Bioassay Mouse
Y Mikami, G Scarno, B Zitti, HY Shih, Y Kanno, A Santoni, JJ O'Shea, G Sciumè NCR+ ILC3 maintain larger STAT4 reservoir via T-BET to regulate type 1 features upon IL-23 stimulation in mice Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29524223] (Bioassay, Mouse) Bioassay Mouse
B Sun, L Zhu, Y Tao, HX Sun, Y Li, P Wang, Y Hou, Y Zhao, X Zhang, L Zhang, N Na, Y Zhao Characterization and allergic role of IL-33-induced neutrophil polarization Cell. Mol. Immunol., 2018;0(0):. 2018 [PMID: 29503441] (Bioassay, Mouse) Bioassay Mouse
PC Duncker, JS Stoolman, AK Huber, BM Segal GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction J. Immunol., 2017;0(0):. 2017 [PMID: 29288202] (Bioassay, Mouse) Bioassay Mouse
T He, Y Huang, C Zhang, D Liu, C Cheng, W Xu, X Zhang Interleukin-17A-promoted MSC2 polarization related with new bone formation of ankylosing spondylitis Oncotarget, 2017;8(57):96993-97008. 2017 [PMID: 29228588] (Bioassay, Mouse) Bioassay Mouse
HA Purvis, F Clarke, CK Jordan, CS Blanco, GH Cornish, X Dai, DJ Rawlings, R Zamoyska, AP Cope Protein Tyrosine Phosphatase PTPN22 regulates IL-1? dependent Th17 responses by modulating dectin-1 signaling in mice Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 28948613] (Bioassay, Mouse) Bioassay Mouse
A Muschaweck, F Petermann, T Korn IL-1? and IL-23 Promote Extrathymic Commitment of CD27(+)CD122(-) ?? T Cells to ??T17 Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28855314] (Bioassay, Mouse) Bioassay Mouse
Show All 118 Publications.

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Bioinformatics

Gene Symbol Il23a
Entrez
Uniprot