Recombinant Mouse IL-23 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications BA

Order Details

Recombinant Mouse IL-23 Protein Summary

Details of Functionality
Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.05-0.25 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived
Mouse IL-23 p40
(Met23-Ser335)
Accession # P43432
IGSGSSRGGSGSGGSGGGGSK Mouse IL-23 p19
(Leu20-Ala196)
Accession # Q9EQ14
N-terminus C-terminus
Accession #
N-terminal Sequence
Met23
Protein/Peptide Type
Recombinant Proteins
Gene
Il23a
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
66 kDa, reducing conditions
Publications
Read Publications using
1887-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-23 Protein

  • IL23
  • IL-23
  • IL23A
  • IL-23A
  • IL-23-A
  • IL-23p19
  • IL23P19P19
  • interleukin 23 p19 subunit
  • interleukin 23, alpha subunit p19
  • interleukin-23 subunit alpha
  • Interleukin-23 subunit p19
  • JKA3 induced upon T-cell activation
  • MGC79388
  • SGRF
  • SGRFIL-23 subunit alpha

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

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Publications for (108)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture Substrate, In Vivo, cell culture.


Filter By Application
Bioassay
(90)
Cell Culture Substrate
(1)
In Vivo
(13)
cell culture
(1)
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Mouse
(105)
All Species
Showing Publications 1 - 10 of 108. Show All 108 Publications.
Publications using 1887-ML Applications Species
HA Purvis, F Clarke, CK Jordan, CS Blanco, GH Cornish, X Dai, DJ Rawlings, R Zamoyska, AP Cope Protein Tyrosine Phosphatase PTPN22 regulates IL-1? dependent Th17 responses by modulating dectin-1 signaling in mice Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 28948613] (Bioassay, Mouse) Bioassay Mouse
A Muschaweck, F Petermann, T Korn IL-1? and IL-23 Promote Extrathymic Commitment of CD27(+)CD122(-) ?? T Cells to ??T17 Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28855314] (Bioassay, Mouse) Bioassay Mouse
Y Arima, T Ohki, N Nishikawa, K Higuchi, M Ota, Y Tanaka, J Nio-Kobaya, M Elfeky, R Sakai, Y Mori, T Kawamoto, A Stofkova, Y Sakashita, Y Morimoto, M Kuwatani, T Iwanaga, Y Yoshioka, N Sakamoto, A Yoshimura, M Takiguchi, S Sakoda, M Prinz, D Kamimura, M Murakami Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit Elife, 2017;6(0):. 2017 [PMID: 28809157] (Bioassay, Mouse) Bioassay Mouse
AK Savage, HE Liang, RM Locksley The Development of Steady-State Activation Hubs between Adult LTi ILC3s and Primed Macrophages in Small Intestine J. Immunol., 2017;0(0):. 2017 [PMID: 28747343] (In Vivo, Mouse) In Vivo Mouse
WF Carson, KA Cavassani, EM Soares, S Hirai, NA Kittan, MA Schaller, MM Scola, A Joshi, A Matsukawa, DM Aronoff, CN Johnson, Y Dou, KA Gallagher, SL Kunkel The STAT4/MLL1 Epigenetic Axis Regulates the Antimicrobial Functions of Murine Macrophages J. Immunol., 2017;0(0):. 2017 [PMID: 28733487] (Bioassay, Mouse) Bioassay Mouse
W Pan, WT Hao, YJ Shen, XY Li, YJ Wang, FF Sun, JH Yin, J Zhang, RX Tang, JP Cao, KY Zheng The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells Parasit Vectors, 2017;10(1):348. 2017 [PMID: 28732522] (Bioassay, Mouse) Bioassay Mouse
A Aki, M Nagasaki, BA Malynn, A Ma, T Kagari Hypomorphic A20 expression confers susceptibility to psoriasis PLoS ONE, 2017;12(6):e0180481. 2017 [PMID: 28658319] (In Vivo, Mouse) In Vivo Mouse
H Dai, F He, GC Tsokos, VC Kyttaris IL-23 Limits the Production of IL-2 and Promotes Autoimmunity in Lupus J. Immunol., 2017;0(0):. 2017 [PMID: 28646040] (Bioassay, Mouse) Bioassay Mouse
SH Kim, BH Cho, H Kiyono, YS Jang Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer&#039;s patches Sci Rep, 2017;7(1):3980. 2017 [PMID: 28638068] (Bioassay, Mouse) Bioassay Mouse
MM Montoya, J Maul, PB Singh, HH Pua, F Dahlström, N Wu, X Huang, KM Ansel, D Baumjohann A Distinct Inhibitory Function for miR-18a in Th17 Cell Differentiation J. Immunol., 2017;0(0):. 2017 [PMID: 28607111] (Bioassay, Mouse) Bioassay Mouse
Show All 108 Publications.

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Bioinformatics

Gene Symbol Il23a
Entrez
Uniprot