Recombinant Mouse IL-23 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-23 Protein, CF Summary

Details of Functionality
Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.05-0.25 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse IL-23 protein
Mouse IL-23 p40
(Met23-Ser335)
Accession # P43432
IGSGSSRGGSGSGGSGGGGSK Mouse IL-23 p19
(Leu20-Ala196)
Accession # Q9EQ14
N-terminus C-terminus
Accession #
N-terminal Sequence
Met23
Protein/Peptide Type
Recombinant Proteins
Gene
Il23a
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
(58-71) kDa, non-reducing conditions
Publications
Read Publications using
1887-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-23 Protein, CF

  • IL-23 p19/IL-12 p40
  • IL23
  • IL-23
  • IL-23A
  • IL-23-A
  • IL-23p19
  • IL-23p19/IL-12p40
  • IL23P19P19
  • interleukin 23 p19 subunit
  • interleukin 23, alpha subunit p19
  • interleukin-23 subunit alpha
  • Interleukin-23 subunit p19
  • JKA3 induced upon T-cell activation
  • MGC79388
  • SGRF
  • SGRFIL-23 subunit alpha

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human Tnbsp;cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.
  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

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Publications for IL-23A/IL-23 P19 (1887-ML/CF)(173)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA Developmet, In Vivo, Organoid Culture.


Filter By Application
Bioassay
(144)
Cell Culture
(7)
ELISA Developmet
(1)
In Vivo
(15)
Organoid Culture
(1)
All Applications
Filter By Species
Mouse
(166)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 173. Show All 173 Publications.
Publications using 1887-ML/CF Applications Species
K Nishida-Ta, A Kimura, T Tsubata, S Takahashi, H Suzuki Antioxidative enzyme NAD(P)H quinone oxidoreductase 1 (NQO1) modulates the differentiation of Th17 cells by regulating ROS levels PLoS ONE, 2022;17(7):e0272090. 2022 [PMID: 35905076] (Bioassay, Mouse) Bioassay Mouse
X Chen, A Jaiswal, Z Costliow, P Herbst, EA Creasey, N Oshiro-Rap, MJ Daly, KL Carey, DB Graham, RJ Xavier pH sensing controls tissue inflammation by modulating cellular metabolism and endo-lysosomal function of immune cells Nature Immunology, 2022;0(0):. 2022 [PMID: 35668320] (Bioassay, Mouse) Bioassay Mouse
YS Zhang, DE Xin, Z Wang, W Peng, Y Zeng, J Liang, M Xu, N Chen, J Zhang, J Yue, M Cao, C Zhang, Y Wang, Z Chang, XM Lu, L Chang, YE Chinn Acetylation licenses Th1 cell polarization to constrain Listeria monocytogenes infection Cell Death and Differentiation, 2022;0(0):. 2022 [PMID: 35614130] (Bioassay, Mouse) Bioassay Mouse
LEA Damasceno, GCM Cebinelli, MF Fernandes, DC Nascimento, GA Públio, MAR Vinolo, SC Oliveira, T Sparwasser, TM Cunha, FQ Cunha, JC Alves-Filh STING is an intrinsic checkpoint inhibitor that restrains the TH17 cell pathogenic program Cell Reports, 2022;39(8):110838. 2022 [PMID: 35613599] (Bioassay, Mouse) Bioassay Mouse
Q Zhang, W Liu, H Wang, H Zhou, K Bulek, X Chen, CJ Zhang, J Zhao, R Zhang, C Liu, Z Kang, RA Bermel, G Dubyak, DW Abbott, TS Xiao, LE Nagy, X Li TH17 cells promote CNS inflammation by sensing danger signals via Mincle Nature Communications, 2022;13(1):2406. 2022 [PMID: 35504893] (Bioassay, Mouse) Bioassay Mouse
HW Cheng, U Mörbe, M Lütge, C Engetschwi, L Onder, M Novkovic, C Gil-Cruz, C Perez-Shib, T Hehlgans, E Scandella, B Ludewig Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function Nature Communications, 2022;13(1):2027. 2022 [PMID: 35440118] (Bioassay, Mouse) Bioassay Mouse
N Yogev, T Bedke, Y Kobayashi, L Brockmann, D Lukas, T Regen, AL Croxford, A Nikolav, N Hövelmeyer, E von Stebut, M Prinz, C Ubeda, KJ Maloy, N Gagliani, RA Flavell, A Waisman, S Huber CD4+ T-cell-derived IL-10 promotes CNS inflammation in mice by sustaining effector T�cell survival Cell Reports, 2022;38(13):110565. 2022 [PMID: 35354043] (Bioassay, Mouse) Bioassay Mouse
M Wakai, R Hayashi, Y Ueno, K Onishi, T Takasago, T Uchida, H Takigawa, R Yuge, Y Urabe, S Oka, Y Kitadai, S Tanaka Promoting mechanism of serum amyloid a family expression in mouse intestinal epithelial cells PLoS ONE, 2022;17(3):e0264836. 2022 [PMID: 35303008] (Organoid Culture, Mouse) Organoid Culture Mouse
S Ma, SA Patel, Y Abe, N Chen, PR Patel, BS Cho, N Abbasi, S Zeng, B Schnabl, JT Chang, WJM Huang RORgammat phosphorylation protects against T�cell-mediated inflammation Cell Reports, 2022;38(11):110520. 2022 [PMID: 35294872] (Cell Culture, Mouse) Cell Culture Mouse
V Patel, A Jayaraman, S Jayaraman Epigenetic drug ameliorated type 1 diabetes via decreased generation of Th1 and Th17 subsets and restoration of self-tolerance in CD4+ T cells International immunopharmacology, 2021;103(0):108490. 2021 [PMID: 34954557] (Bioassay, Mouse) Bioassay Mouse
Show All 173 Publications.

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Bioinformatics

Gene Symbol Il23a
Uniprot