Recombinant Mouse IL-1 alpha/IL-1F1 Protein

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1 μg/lane of Recombinant Mouse IL‑1 alpha /IL‑1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.
Recombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 3-7 pg/mL.

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-1 alpha/IL-1F1 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 3-7 pg/mL.
Source
E. coli-derived mouse IL-1 alpha/IL-1F1 protein
Ser6-Ser161
Accession #
N-terminal Sequence
Ser6
Protein/Peptide Type
Recombinant Proteins
Gene
Il1a
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
400-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

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Publications for IL-1 alpha/IL-1F1 (400-ML)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(12)
In Vivo
(4)
All Applications
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Human
(1)
Mouse
(15)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 400-ML Applications Species
PC Duncker, JS Stoolman, AK Huber, BM Segal GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction J. Immunol., 2017;0(0):. 2017 [PMID: 29288202] (Bioassay, Mouse) Bioassay Mouse
Jiang H, Promchan K, Lin B, Lockett S, Chen D, Marshall H, Badralmaa Y, Natarajan V LZTFL1 Upregulated by All-Trans Retinoic Acid during CD4+ T Cell Activation Enhances IL-5 Production. J Immunol, 2015;196(3):1081-90. 2015 [PMID: 26700766] (Bioassay, Mouse) Bioassay Mouse
Jules J, Wang S, Shi Z, Liu J, Wei S, Feng X The IVVY Motif and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Sites in the Cytoplasmic Domain of the Receptor Activator of Nuclear Factor kappaB (RANK) Cooperate to Induce Osteoclastogenesis. J Biol Chem, 2015;290(39):23738-50. 2015 [PMID: 26276390] (Bioassay, Mouse) Bioassay Mouse
Lin D, Lei L, Zhang Y, Hu B, Bao G, Liu Y, Song Y, Liu C, Wu Y, Zhao L, Yu X, Liu H Secreted IL-1alpha promotes T-cell activation and expansion of CD11b(+) Gr1(+) cells in carbon tetrachloride-induced liver injury in mice. Eur J Immunol, 2015;45(7):2084-98. 2015 [PMID: 25870999] (Bioassay, Mouse) Bioassay Mouse
Rabeony H, Petit-Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, Jegou J, Guillet G, Huguier V, Lecron J, Bernard F, Morel F Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M. PLoS ONE, 2014;9(7):e101937. 2014 [PMID: 25010647] (In Vivo, Mouse) In Vivo Mouse
Szewczyk K, Fuller K, Chambers T Distinctive subdomains in the resorbing surface of osteoclasts. PLoS ONE, 2013;8(3):e60285. 2013 [PMID: 23555944] (Bioassay, Mouse) Bioassay Mouse
Jayaraman P, Sada-Ovalle I, Nishimura T, Anderson A, Kuchroo V, Remold H, Behar S IL-1beta promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation. J Immunol, 2013;190(8):4196-204. 2013 [PMID: 23487424] (Bioassay, Mouse) Bioassay Mouse
Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J. Exp. Med., 2012;209(8):1505-17. 2012 [PMID: 22802353] (Bioassay, Mouse) Bioassay Mouse
Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010;84(24):12703-12. 2010 [PMID: 20881038] (In Vivo, Mouse) In Vivo Mouse
Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis. J. Immunol., 2010;184(0):5263-70. 2010 [PMID: 20335534] (In Vivo, Mouse) In Vivo Mouse
Show All 16 Publications.

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Bioinformatics

Gene Symbol Il1a
Entrez
Uniprot