Recombinant Mouse IL-1 alpha/IL-1F1 Protein

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Recombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 3-7 pg/mL.
1 μg/lane of Recombinant Mouse IL‑1 alpha /IL‑1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-1 alpha/IL-1F1 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 3-7 pg/mL.
Source
E. coli-derived mouse IL-1 alpha/IL-1F1 protein
Ser6-Ser161
Accession #
N-terminal Sequence
Ser6
Protein/Peptide Type
Recombinant Proteins
Gene
Il1a
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
400-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta , that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta , designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Publications for IL-1 alpha/IL-1F1 (400-ML)(24)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, IHC, In Vivo.


Filter By Application
Bioassay
(17)
Cell Culture
(1)
IHC
(1)
In Vivo
(6)
All Applications
Filter By Species
Human
(1)
Mouse
(24)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using 400-ML Applications Species
LaMarche, NM;Hegde, S;Park, MD;Maier, BB;Troncoso, L;Le Berichel, J;Hamon, P;Belabed, M;Mattiuz, R;Hennequin, C;Chin, T;Reid, AM;Reyes-Torres, I;Nemeth, E;Zhang, R;Olson, OC;Doroshow, DB;Rohs, NC;Gomez, JE;Veluswamy, R;Hall, N;Venturini, N;Ginhoux, F;Liu, Z;Buckup, M;Figueiredo, I;Roudko, V;Miyake, K;Karasuyama, H;Gonzalez-Kozlova, E;Gnjatic, S;Passegué, E;Kim-Schulze, S;Brown, BD;Hirsch, FR;Kim, BS;Marron, TU;Merad, M; An IL-4 signalling axis in bone marrow drives pro-tumorigenic myelopoiesis Nature 2023-12-06 [PMID: 38057662] (Bioassay, Mouse) Bioassay Mouse
Wang, Y;Wu, T;Tsai, MC;Rezzonico, MG;Abdel-Haleem, AM;Xie, L;Gandham, VD;Ngu, H;Stark, K;Glock, C;Xu, D;Foreman, O;Friedman, BA;Sheng, M;Hanson, JE; TPL2 kinase activity regulates microglial inflammatory responses and promotes neurodegeneration in tauopathy mice eLife 2023-08-09 [PMID: 37555828] (Bioassay, Mouse) Bioassay Mouse
A Matsunami, M Mizuno-Kam, H Kawaki, E Takayama, K Ueno, M Ando, H Morimoto-I, Y Muramatsu, S Sumitomo, N Kondoh Augmented secretion of IL-1&amp;alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells Journal of oral biosciences, 2021-06-18;0(0):. 2021-06-18 [PMID: 34153475] (IHC, Mouse) IHC Mouse
KE Salmeron, ME Maniskas, DN Edwards, R Wong, I Rajkovic, A Trout, AA Rahman, S Hamilton, JF Fraser, E Pinteaux, GJ Bix Interleukin 1 alpha administration is neuroprotective and neuro-restorative following experimental ischemic stroke J Neuroinflammation, 2019-11-14;16(1):222. 2019-11-14 [PMID: 31727174] (In Vivo, Mouse) In Vivo Mouse
N Ketelut-Ca, COS Souza, L Benevides, LG Gardinassi, MC Silva, LA Tavares, DS Zamboni, JS Silva Caspase-11-dependent IL-1α release boosts Th17 immunity against Paracoccidioides brasiliensis PLoS Pathog., 2019-08-19;15(8):e1007990. 2019-08-19 [PMID: 31425553] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
H Lindahl, AO Guerreiro-, SK Bedri, M Linnerbaue, M Lindén, N Abdelmagid, K Tandre, C Hollins, L Irving, C Glover, C Jones, L Alfredsson, L Rönnblom, I Kockum, M Khademi, M Jagodic, T Olsson IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis J. Immunol., 2019-07-10;0(0):. 2019-07-10 [PMID: 31292217] (Cell Culture, Mouse) Cell Culture Mouse
X Liu, Y Sun, Y Zheng, M Zhang, X Jin, K Kang, Y Wang, S Li, H Zhang, Q Zhao, S Zhang, J Wu, B Yu Administration of Interleukin-35-Conditioned Autologous Tolerogenic Dendritic Cells Prolong Allograft Survival After Heart Transplantation Cell. Physiol. Biochem., 2018-09-07;49(3):1180-1196. 2018-09-07 [PMID: 30196277] (Bioassay, Mouse) Bioassay Mouse
PC Duncker, JS Stoolman, AK Huber, BM Segal GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction J. Immunol., 2017-12-29;0(0):. 2017-12-29 [PMID: 29288202] (Bioassay, Mouse) Bioassay Mouse
Jiang H, Promchan K, Lin B, Lockett S, Chen D, Marshall H, Badralmaa Y, Natarajan V LZTFL1 Upregulated by All-Trans Retinoic Acid during CD4+ T Cell Activation Enhances IL-5 Production. J Immunol, 2015-12-23;196(3):1081-90. 2015-12-23 [PMID: 26700766] (Bioassay, Mouse) Bioassay Mouse
Jules J, Wang S, Shi Z, Liu J, Wei S, Feng X The IVVY Motif and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Sites in the Cytoplasmic Domain of the Receptor Activator of Nuclear Factor kappaB (RANK) Cooperate to Induce Osteoclastogenesis. J Biol Chem, 2015-08-14;290(39):23738-50. 2015-08-14 [PMID: 26276390] (Bioassay, Mouse) Bioassay Mouse
Show All 24 Publications.

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Bioinformatics

Gene Symbol Il1a
Uniprot