Recombinant Mouse IL-1 alpha/IL-1F1 Protein, CF

Recombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 3-7 pg/mL.

1 μg/lane of Recombinant Mouse IL‑1 alpha /IL‑1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-1 alpha/IL-1F1 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is 3-7 pg/mL.
• Source: E. coli-derived mouse IL-1 alpha/IL-1F1 protein
  Ser6-Ser161
• Accession #: Q62161
• N-terminal Sequence: Ser6
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il1a
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 50 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-1 alpha/IL-1F1 Protein, CF

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta , that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta , designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Publications for IL-1 alpha/IL-1F1 (400-ML/CF)(22)

Publications using 400-ML/CF

• A Matsunami, M Mizuno-Kam, H Kawaki, E Takayama, K Ueno, M Ando, H Morimoto-I, Y Muramatsu, S Sumitomo, N Kondoh Augmented secretion of IL-1&amp;alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells Journal of oral biosciences, 2021-06-18;0(0):. 2021-06-18 [PMID: 34153475] (IHC, Mouse)
• KE Salmeron, ME Maniskas, DN Edwards, R Wong, I Rajkovic, A Trout, AA Rahman, S Hamilton, JF Fraser, E Pinteaux, GJ Bix Interleukin 1 alpha administration is neuroprotective and neuro-restorative following experimental ischemic stroke J Neuroinflammation, 2019-11-14;16(1):222. 2019-11-14 [PMID: 31727174] (In Vivo, Mouse)
• N Ketelut-Ca, COS Souza, L Benevides, LG Gardinassi, MC Silva, LA Tavares, DS Zamboni, JS Silva Caspase-11-dependent IL-1α release boosts Th17 immunity against Paracoccidioides brasiliensis PLoS Pathog., 2019-08-19;15(8):e1007990. 2019-08-19 [PMID: 31425553] (Bioassay, In Vivo, Mouse)
• H Lindahl, AO Guerreiro-, SK Bedri, M Linnerbaue, M Lindén, N Abdelmagid, K Tandre, C Hollins, L Irving, C Glover, C Jones, L Alfredsson, L Rönnblom, I Kockum, M Khademi, M Jagodic, T Olsson IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis J. Immunol., 2019-07-10;0(0):. 2019-07-10 [PMID: 31292217] (Cell Culture, Mouse)
• X Liu, Y Sun, Y Zheng, M Zhang, X Jin, K Kang, Y Wang, S Li, H Zhang, Q Zhao, S Zhang, J Wu, B Yu Administration of Interleukin-35-Conditioned Autologous Tolerogenic Dendritic Cells Prolong Allograft Survival After Heart Transplantation Cell. Physiol. Biochem., 2018-09-07;49(3):1180-1196. 2018-09-07 [PMID: 30196277] (Bioassay, Mouse)
• PC Duncker, JS Stoolman, AK Huber, BM Segal GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction J. Immunol., 2017-12-29;0(0):. 2017-12-29 [PMID: 29288202] (Bioassay, Mouse)
• Jiang H, Promchan K, Lin B, Lockett S, Chen D, Marshall H, Badralmaa Y, Natarajan V LZTFL1 Upregulated by All-Trans Retinoic Acid during CD4+ T Cell Activation Enhances IL-5 Production. J Immunol, 2015-12-23;196(3):1081-90. 2015-12-23 [PMID: 26700766] (Bioassay, Mouse)
• Jules J, Wang S, Shi Z, Liu J, Wei S, Feng X The IVVY Motif and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Sites in the Cytoplasmic Domain of the Receptor Activator of Nuclear Factor kappaB (RANK) Cooperate to Induce Osteoclastogenesis. J Biol Chem, 2015-08-14;290(39):23738-50. 2015-08-14 [PMID: 26276390] (Bioassay, Mouse)
• Lin D, Lei L, Zhang Y, Hu B, Bao G, Liu Y, Song Y, Liu C, Wu Y, Zhao L, Yu X, Liu H Secreted IL-1alpha promotes T-cell activation and expansion of CD11b(+) Gr1(+) cells in carbon tetrachloride-induced liver injury in mice. Eur J Immunol, 2015-04-29;45(7):2084-98. 2015-04-29 [PMID: 25870999] (Bioassay, Mouse)
• Rabeony H, Petit-Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, Jegou J, Guillet G, Huguier V, Lecron J, Bernard F, Morel F Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M. PLoS ONE, 2014-07-10;9(7):e101937. 2014-07-10 [PMID: 25010647] (In Vivo, Mouse)
• Chen D, Goswami C, Burnett R, Anjanappa M, Bhat-Nakshatri P, Muller W, Nakshatri H Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle. Cancer Res, 2014-06-30;74(16):4270-81. 2014-06-30 [PMID: 24980554] (Bioassay, Mouse)
• Szewczyk K, Fuller K, Chambers T Distinctive subdomains in the resorbing surface of osteoclasts. PLoS ONE, 2013-03-21;8(3):e60285. 2013-03-21 [PMID: 23555944] (Bioassay, Mouse)
• Jayaraman P, Sada-Ovalle I, Nishimura T, Anderson A, Kuchroo V, Remold H, Behar S IL-1beta promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation. J Immunol, 2013-03-13;190(8):4196-204. 2013-03-13 [PMID: 23487424] (Bioassay, Mouse)
• Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J. Exp. Med., 2012-07-16;209(8):1505-17. 2012-07-16 [PMID: 22802353] (Bioassay, Mouse)
• Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010-09-29;84(24):12703-12. 2010-09-29 [PMID: 20881038] (In Vivo, Mouse)
• Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis. J. Immunol., 2010-03-24;184(0):5263-70. 2010-03-24 [PMID: 20335534] (In Vivo, Mouse)
• Dienz O, Eaton SM, Bond JP, Neveu W, Moquin D, Noubade R, Briso EM, Charland C, Leonard WJ, Ciliberto G, Teuscher C, Haynes L, Rincon M The induction of antibody production by IL-6 is indirectly mediated by IL-21 produced by CD4+ T cells. J. Exp. Med., 2009-01-12;206(1):69-78. 2009-01-12 [PMID: 19139170] (Bioassay, Mouse)
• Chackerian AA, Oldham ER, Murphy EE, Schmitz J, Pflanz S, Kastelein RA IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol., 2007-08-15;179(4):2551-5. 2007-08-15 [PMID: 17675517] (Bioassay, Mouse)
• Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H Protection of CD8+ T cells from activation-induced cell death by IL-18. J. Leukoc. Biol., 2007-03-30;82(1):142-51. 2007-03-30 [PMID: 17400610] (Bioassay, Mouse)
• Wei S, Kitaura H, Zhou P, Ross FP, Teitelbaum SL IL-1 mediates TNF-induced osteoclastogenesis. J. Clin. Invest., 2005-02-01;115(2):282-90. 2005-02-01 [PMID: 15668736] (Bioassay, Human, Mouse)
• Scharstuhl A, van Beuningen HM, Vitters EL, van der Kraan PM, van den Berg WB Loss of transforming growth factor counteraction on interleukin 1 mediated effects in cartilage of old mice. Ann. Rheum. Dis., 2002-12-01;61(12):1095-8. 2002-12-01 [PMID: 12429542] (In Vivo, Mouse)
• Coope HJ, Atkinson PG, Huhse B, Belich M, Janzen J, Holman MJ, Klaus GG, Johnston LH, Ley SC CD40 regulates the processing of NF-kappaB2 p100 to p52. EMBO J., 2002-10-15;21(20):5375-85. 2002-10-15 [PMID: 12374738] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Il1a
• Uniprot:
  • Q62161()