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400-ML), Recombinant Human IL-1a/IL-1F1 (200-LA) and Recombinant Rat IL-1a/IL-1F1 (500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for IL-1a/IL-1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA). The ND50 is typically 0.002‑0.004 µg/mL." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
400-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB400) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB200). The ND50 is typically 0.05-0.15 µg/mL." class="big_lightbox" target="_blank">
200-LA) was serially diluted and captured by Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB200) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA). The ND50 is typically 0.75-4.5 ng/mL." class="big_lightbox" target="_blank">
200-LA) was serially diluted and captured by Mouse Anti-Human IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB200) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.
![Western Blot IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL1_alpha_AF400NA_Western_Blot_21575.jpg)
![Neutralization IL-1 alpha/IL-1F1 Antibody (4414) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-1_alpha_MAB200_Block_Neutralize_9050.jpg)
![Neutralization IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-1_alpha_AF-200-NA_Block_Neutralize_8638.jpg)
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![N/A IL-1 alpha/IL-1F1 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL1_alpha_DLA50_ELISA_735.jpg)
![N/A IL-1 alpha/IL-1F1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL1_alpha_DY400_ELISA_1998.jpg)
![N/A IL-1 alpha/IL-1F1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL1_alpha_DY200_ELISA_1748.jpg)
![Immunohistochemistry IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL1_alpha_AF400NA_Immunohistochemistry_17353.jpg)
![Neutralization IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-1_alpha_AF-400-NA_Block_Neutralize_8699.jpg)
![Immunohistochemistry IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af-400-na_mouse-il-1-alpha-il-1f1-affinity-purified-polyclonal-ab-immunohistochemistry-762023133318.jpg)
![Immunocytochemistry/ Immunofluorescence IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-400-na_mouse-il-1-alpha-il-1f1-affinity-purified-polyclonal-ab-8220249504612.jpg)
![Immunocytochemistry/ Immunofluorescence IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-400-na_mouse-il-1-alpha-il-1f1-affinity-purified-polyclonal-ab-44202415425064.jpg)
![ELISA IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/images/af-400-na_mouse-il-1-alpha-il-1f1-affinity-purified-polyclonal-ab-elisa-639201852917429517.jpg)
![Immunocytochemistry IL-1 alpha/IL-1F1 Antibody (4414) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-1_alpha_MAB200_Immunocytochemistry_6581.jpg)
![Immunohistochemistry IL-1 alpha/IL-1F1 Antibody (4414) [Unconjugated]](http://images.novusbio.com/fullsize/mab200_human-il-1-alpha-il-1f1-mab-clone-4414-immunohistochemistry-12122025743723.jpg)
![ELISA IL-1 alpha/IL-1F1 Antibody (4414) [Unconjugated]](http://images.novusbio.com/images/mab200_human-il-1-alpha-il-1f1-mab-clone-4414-elisa-639201775911376903.jpg)
![Immunocytochemistry IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-1_alpha_AF-200-NA_Immunocytochemistry_9334.jpg)
![Functional IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-200-na_human-il-1-alpha-il-1f1-affinity-purified-polyclonal-ab-8220249503263.jpg)
![ELISA IL-1 alpha/IL-1F1 Antibody [Unconjugated]](http://images.novusbio.com/images/af-200-na_human-il-1-alpha-il-1f1-affinity-purified-polyclonal-ab-elisa-639201852917414571.jpg)
![N/A IL-1 alpha/IL-1F1 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL1_alpha_DLA50_ELISA_736.jpg)
![N/A IL-1 alpha/IL-1F1 [HRP]](http://images.novusbio.com/fullsize/elisa/IL-1_alpha_DLA50_ELISA_128.jpg)
