Recombinant Mouse FGF-10 Protein

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse FGF-10 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 20-120 ng/mL
Source
E. coli-derived mouse FGF-10 protein
Ser62-Thr209
Accession #
N-terminal Sequence
Ser62
Protein/Peptide Type
Recombinant Proteins
Gene
Fgf10
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
6224-FG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF-10 Protein

  • FGF10
  • FGF-10
  • fibroblast growth factor 10
  • Keratinocyte growth factor 2
  • KGF-2
  • produced by fibroblasts of urinary bladder lamina propria

Background

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature mouse FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). C-terminal to this region (aa 62 ‑ 209), it shares  94% and 100% amino acid sequence identity with human and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (2, 8), formation of the otic vesicle and chochlea (9), adipogenesis (10), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (11 - 14). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 9, 12 ‑ 14). The expression and function of FGF‑10 are negatively regulated by Shh and BMP-4 in the developing lung (2, 8). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF-10 biology (7, 9, 13, 14). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (15).
  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Bellusci, S. et al. (1997) Development 124:4867.
  3. Tagashira, S. et al. (1997) Gene 197:399.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Weaver, M. et al. (2000) Development 127:2695.
  9. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  10. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  11. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  12. Mailleux, A.A. et al. (2002) Development 129:53.
  13. Makarenkova, H.P. et al. (2000) Development 127:2563.
  14. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  15. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

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6224-FG
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Publications for FGF-10 (6224-FG)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture Substrate, In Vivo.


Filter By Application
Bioassay
(11)
Cell Culture Substrate
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(12)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 6224-FG Applications Species
MA Scavuzzo, D Yang, M Borowiak Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells Sci Rep, 2017;7(1):10810. 2017 [PMID: 28883507] (Bioassay, Mouse) Bioassay Mouse
L Chatzeli, M Gaete, AS Tucker Fgf10-Sox9 are essential for establishment of distal progenitor cells during salivary gland development Development, 2017;0(0):. 2017 [PMID: 28506998] (Bioassay, Mouse) Bioassay Mouse
N Cerdá-Este, H Naumann, S Ruzittu, N Mah, IM Pongrac, C Cozzitorto, A Hommel, MA Andrade-Na, E Bonifacio, FM Spagnoli Stepwise reprogramming of liver cells to a pancreas progenitor state by the transcriptional regulator Tgif2 Nat Commun, 2017;8(0):14127. 2017 [PMID: 28193997] (Bioassay, Mouse) Bioassay Mouse
KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells Wnt/?-catenin promotes gastric fundus specification in mice and humans Nature, 2017;0(0):. 2017 [PMID: 28052057] (Bioassay, Mouse) Bioassay Mouse
Tissue-Specific Stem Cells Obtained by Reprogramming of Non-Obese Diabetic (NOD) Mouse-Derived Pancreatic Cells Confer Insulin Production in Response to Glucose PLoS ONE, 2016;11(9):e0163580. 2016 [PMID: 27662374] (Bioassay, Mouse) Bioassay Mouse
Jennifer Quantius Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair PLoS Pathog, 2016;12(6):e1005544. 2016 [PMID: 27322618] (In Vivo, Mouse) In Vivo Mouse
Marie-Isabelle Garcia Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage Development, 2016;143(9):1452-63. 2016 [PMID: 26989172] (Bioassay, Mouse) Bioassay Mouse
Chan W, Howe K, Clegg J, Guimond S, Price D, Turnbull J, Pratt T 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline. PLoS ONE, 2015;10(6):e0130147. 2015 [PMID: 26075383] (Bioassay, Mouse) Bioassay Mouse
Yang Z, Balic A, Michon F, Juuri E, Thesleff I Mesenchymal Wnt/beta-Catenin Signaling Controls Epithelial Stem Cell Homeostasis in Teeth by Inhibiting the Antiapoptotic Effect of Fgf10. Stem Cells, 2015;33(5):1670-81. 2015 [PMID: 25693510] (Bioassay, Mouse) Bioassay Mouse
Noguchi, H, Saitoh, I, Tsugata, T, Kataoka, H, Watanabe, M, Noguchi, Y Induction of tissue-specific stem cells by reprogramming factors, and tissue-specific selection. Cell Death Differ, 2015;22(1):145-55. 2015 [PMID: 25190146]
Show All 14 Publications.

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Bioinformatics

Gene Symbol Fgf10
Entrez
Uniprot