Recombinant Mouse FGF-10 Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse FGF-10 Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED₅₀ for this effect is 20-120 ng/mL
• Source: E. coli-derived mouse FGF-10 protein
  Ser62-Thr209
• Accession #: NP_032028.1
• N-terminal Sequence: Ser62
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fgf10
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF-10 Protein

• FGF10
• FGF-10
• fibroblast growth factor 10
• Keratinocyte growth factor 2
• KGF2
• KGF-2
• produced by fibroblasts of urinary bladder lamina propria

Background

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature mouse FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). C-terminal to this region (aa 62 ‑ 209), it shares  94% and 100% amino acid sequence identity with human and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (2, 8), formation of the otic vesicle and chochlea (9), adipogenesis (10), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (11 - 14). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 9, 12 ‑ 14). The expression and function of FGF‑10 are negatively regulated by Shh and BMP-4 in the developing lung (2, 8). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF-10 biology (7, 9, 13, 14). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (15).

1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
2. Bellusci, S. et al. (1997) Development 124:4867.
3. Tagashira, S. et al. (1997) Gene 197:399.
4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
6. Min, H. et al. (1998) Genes Dev. 12:3156.
7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
8. Weaver, M. et al. (2000) Development 127:2695.
9. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
10. Sakaue, H. et al. (2002) Genes Dev. 16:908.
11. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
12. Mailleux, A.A. et al. (2002) Development 129:53.
13. Makarenkova, H.P. et al. (2000) Development 127:2563.
14. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
15. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

Publications for FGF-10 (6224-FG)(31)

Publications using 6224-FG

• Ji, X;Sun, Z;Wu, H;Zhang, J;Liu, S;Cao, X;Wang, B;Wang, F;Zhang, Y;Li, B;Feng, J;Zhao, H; More powerful dysregulation of Helicobacter pylori East Asian-type CagA on intracellular signalings BMC microbiology 2024-11-11 [PMID: 39528935] (Bioassay, Mouse)
• Patel, VN;Aure, MH;Choi, SH;Ball, JR;Lane, ED;Wang, Z;Xu, Y;Zheng, C;Liu, X;Martin, D;Pailin, JY;Prochazkova, M;Kulkarni, AB;van Kuppevelt, TH;Ambudkar, IS;Liu, J;Hoffman, MP; Specific 3-O-sulfated heparan sulfate domains regulate salivary gland basement membrane metabolism and epithelial differentiation Nature communications 2024-08-31 [PMID: 39217171] (Immunohistochemistry, Human)
• Aure, MH;Symonds, JM;Villapudua, CU;Dodge, JT;Werner, S;Knosp, WM;Hoffman, MP; FGFR2 is essential for salivary gland duct homeostasis and MAPK-dependent seromucous acinar cell differentiation Nature communications 2023-10-14 [PMID: 37838739] (Bioassay, Mouse)
• DG Brownfield, AD de Arce, E Ghelfi, A Gillich, TJ Desai, MA Krasnow Alveolar cell fate selection and lifelong maintenance of AT2 cells by FGF signaling Nature Communications, 2022-11-21;13(1):7137. 2022-11-21 [PMID: 36414616] (Bioassay, Mouse)
• H Kim, BW Song, SJ Park, SW Choi, H Moon, KC Hwang, SW Kang, SH Moon, Y Yang, IC Kwon, SH Kim Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytes Science Advances, 2022-02-25;8(8):eabj6621. 2022-02-25 [PMID: 35213232] (Bioassay, Mouse)
• N Hosaka, S Kanda, T Shimono, T Nishiyama Induction of gammadeltaT cells from HSC-enriched BMCs co-cultured with iPSC-derived thymic epithelial cells Journal of Cellular and Molecular Medicine, 2021-10-23;0(0):. 2021-10-23 [PMID: 34687276] (Cell Culture, Mouse)
• H Tomita, K Tanaka, A Hirata, H Okada, H Imai, Y Shirakami, K Ohnishi, S Sugie, H Aoki, Y Hatano, K Noguchi, T Kanayama, A Niwa, N Suzui, T Miyazaki, T Tanaka, H Akiyama, M Shimizu, K Yoshida, A Hara Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas Cell Reports, 2021-02-23;34(8):108772. 2021-02-23 [PMID: 33626352] (Mouse)
• N Sankoda, W Tanabe, A Tanaka, H Shibata, K Woltjen, T Chiba, H Haga, Y Sakai, M Mandai, T Yamamoto, Y Yamada, S Uemoto, Y Kawaguchi Epithelial expression of Gata4 and Sox2 regulates specification of the squamous-columnar junction via MAPK/ERK signaling in mice Nature Communications, 2021-01-25;12(1):560. 2021-01-25 [PMID: 33495473] (Bioassay, Mouse)
• H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction Cell Stem Cell, 2020-10-21;0(0):. 2020-10-21 [PMID: 33128895] (Bioassay, Mouse)
• Y Kiyokawa, M Sato, H Noguchi, E Inada, Y Iwase, N Kubota, T Sawami, M Terunuma, T Maeda, H Hayasaki, I Saitoh Drug-Induced Na�ve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic &amp;beta-Cell Lineage Journal of Clinical Medicine, 2020-09-02;9(9):. 2020-09-02 [PMID: 32887316] (Bioassay, Human)
• KA Lloyd, BN Parsons, MD Burkitt, AR Moore, S Papoutsopo, M Boyce, CA Duckworth, K Exarchou, N Howes, L Rainbow, Y Fang, C Oxvig, S Dodd, A Varro, N Hall, DM Pritchard Netazepide Inhibits Expression of Pappalysin 2 in Type-1 Gastric Neuroendocrine Tumors Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):. 2020-01-28 [PMID: 32004755] (Bioassay, Mouse)
• T Momozane, E Fukui, S Funaki, M Fujii, Y Kinehara, E Ito, S Miyagawa, Y Ohno, Y Sawa, M Okumura, Y Shintani Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor Stem Cells Int, 2019-12-27;2019(0):1271682. 2019-12-27 [PMID: 31949433] (Bioassay, Mouse)
• I Guerriero, MT De Angelis, F D'Angelo, R Leveque, E Savignano, L Roberto, V Lucci, P Mazzone, S Laurino, G Storto, A Nardelli, A Sgambato, M Ceccarelli, M De Felice, E Amendola, G Falco Exploring the Molecular Crosstalk between Pancreatic Bud and Mesenchyme in Embryogenesis: Novel Signals Involved Int J Mol Sci, 2019-10-03;20(19):. 2019-10-03 [PMID: 31623299] (Cell Culture, Mouse)
• T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals Nat Commun, 2019-10-11;10(1):4647. 2019-10-11 [PMID: 31604927] (Bioassay, Mouse)
• S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes Sci Rep, 2019-06-11;9(1):8470. 2019-06-11 [PMID: 31186471] (Bioassay, Mouse)
• C Miyagi-Shi, Y Nakashima, N Kobayashi, I Saitoh, M Watanabe, H Noguchi Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA Sci Rep, 2018-08-17;8(1):12341. 2018-08-17 [PMID: 30120295] (Bioassay, Mouse)
• C Zhong, Z Chen, X Luo, C Wang, H Jiang, J Shao, M Guan, L Huang, X Huang, J Wang Barhl1 is required for the differentiation of inner ear hair cell-like cells from mouse embryonic stem cells Int. J. Biochem. Cell Biol., 2018-02-06;96(0):79-89. 2018-02-06 [PMID: 29413750] (Cell Culture, Mouse)
• MA Scavuzzo, D Yang, M Borowiak Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells Sci Rep, 2017-09-07;7(1):10810. 2017-09-07 [PMID: 28883507] (Bioassay, Mouse)
• L Chatzeli, M Gaete, AS Tucker Fgf10-Sox9 are essential for establishment of distal progenitor cells during salivary gland development Development, 2017-05-15;0(0):. 2017-05-15 [PMID: 28506998] (Bioassay, Mouse)
• N Cerdá-Este, H Naumann, S Ruzittu, N Mah, IM Pongrac, C Cozzitorto, A Hommel, MA Andrade-Na, E Bonifacio, FM Spagnoli Stepwise reprogramming of liver cells to a pancreas progenitor state by the transcriptional regulator Tgif2 Nat Commun, 2017-02-13;8(0):14127. 2017-02-13 [PMID: 28193997] (Bioassay, Mouse)
• KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells Wnt/?-catenin promotes gastric fundus specification in mice and humans Nature, 2017-01-04;0(0):. 2017-01-04 [PMID: 28052057] (Bioassay, Mouse)
• Tissue-Specific Stem Cells Obtained by Reprogramming of Non-Obese Diabetic (NOD) Mouse-Derived Pancreatic Cells Confer Insulin Production in Response to Glucose PLoS ONE, 2016-09-23;11(9):e0163580. 2016-09-23 [PMID: 27662374] (Bioassay, Mouse)
• Jennifer Quantius Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair PLoS Pathog, 2016-06-20;12(6):e1005544. 2016-06-20 [PMID: 27322618] (Bioassay, In Vivo, Mouse)
• Marie-Isabelle Garcia Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage Development, 2016-03-17;143(9):1452-63. 2016-03-17 [PMID: 26989172] (Bioassay, Mouse)
• Chan W, Howe K, Clegg J, Guimond S, Price D, Turnbull J, Pratt T 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline. PLoS ONE, 2015-06-15;10(6):e0130147. 2015-06-15 [PMID: 26075383] (Bioassay, Mouse)
• Yang Z, Balic A, Michon F, Juuri E, Thesleff I Mesenchymal Wnt/beta-Catenin Signaling Controls Epithelial Stem Cell Homeostasis in Teeth by Inhibiting the Antiapoptotic Effect of Fgf10. Stem Cells, 2015-05-01;33(5):1670-81. 2015-05-01 [PMID: 25693510] (Bioassay, Mouse)
• Noguchi , H, Saitoh , I, Tsugata , T, Kataoka , H, Watanabe , M, Noguchi , Y Induction of tissue-specific stem cells by reprogramming factors, and tissue-specific selection. Cell Death Differ, 2014-09-05;22(1):145-55. 2014-09-05 [PMID: 25190146] (Bioassay, Mouse)
• Cai Z, Grobe K, Zhang X Role of heparan sulfate proteoglycans in optic disc and stalk morphogenesis. Dev Dyn, 2014-05-06;243(10):1310-6. 2014-05-06 [PMID: 24753163] (Bioassay, Mouse)
• Song Z, Liu C, Iwata J, Gu S, Suzuki A, Sun C, He W, Shu R, Li L, Chai Y, Chen Y Mice with Tak1 deficiency in neural crest lineage exhibit cleft palate associated with abnormal tongue development. J Biol Chem, 2013-03-04;288(15):10440-50. 2013-03-04 [PMID: 23460641] (Bioassay, Mouse)
• Sneddon , Julie B, Borowiak , Malgorza, Melton , Douglas Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 2012-10-07;491(7426):765-8. 2012-10-07 [PMID: 23041930] (Cell Culture, Human)
• Rebustini IT, Hayashi T, Reynolds AD miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis. Development, 2011-11-24;139(1):191-202. 2011-11-24 [PMID: 22115756] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Fgf10
• Uniprot:
  • NP_032028.1()