Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109)

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109) Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED₅₀ for this effect is 1-5 ng/mL.
• Source: E. coli-derived mouse CXCL7/Thymus Chemokine-1 protein
  Ile48-Ile109
• Accession #: Q9EQI5
• N-terminal Sequence: Ile48
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ppbp
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 6.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109)

• Beta-TG;B-TG1;CTAP3;CTAPIII;CTAP-III;CXCL7;LA-PF4;LDGF;MDGF;NAP-2;PBP;Platelet basic protein;PPBP;SCYB7;TC1;TC2;TGB;TGB1;THBGB;THBGB1
• Thymus Chemokine1
• Thymus Chemokine-1

Background

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein that shares 72% amino acid sequence identity with rat CXCL7 (accession number AAK30166). The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract human CXCR2 transfected mouse BaF/3 cells, but not human CXCR1 transfected mouse BaF3 cells. Two isoforms of mouse CXCL7 have been produced at R&D Systems. The Ile48 - Ile109 mouse CXCL7 isoform (R&D Systems, Catalog # 1091-CK) has been shown to be 200-fold more potent than the Lys40 - Tyr113 isoform (R&D Systems, Catalog # 763-CK) as a chemoattractant for mouse BaF/3 cells transfected with human CXCR2.

Publications for CXCL7/Thymus Chemokine-1 (1091-CK)(1)

Publication using 1091-CK

• Graca, FA;Stephan, A;Minden-Birkenmaier, BA;Shirinifard, A;Wang, YD;Demontis, F;Labelle, M; Platelet-derived chemokines promote skeletal muscle regeneration by guiding neutrophil recruitment to injured muscles Nature communications 2023-05-22 [PMID: 37217480] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ppbp
• Uniprot:
  • Q9EQI5()