Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 40-113)

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 40-113) Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2. The ED 50 for this effect is 0.2-1 µg/mL.
• Source: E. coli-derived mouse CXCL7/Thymus Chemokine-1 protein Lys40-Tyr113
• Accession #: AAG36786
• N-terminal Sequence: Lys40
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ppbp
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 40-113)

• Beta-TG;B-TG1;CTAP3;CTAPIII;CTAP-III;CXCL7;LA-PF4;LDGF;MDGF;NAP-2;PBP;Platelet basic protein;PPBP;SCYB7;TC1;TC2;TGB;TGB1;THBGB;THBGB1
• Thymus Chemokine1
• Thymus Chemokine-1

Background

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein. Signal P prediction program analysis indicates that this precursor protein contains an amino terminal 39 amino acid residue signal peptide and a 74 amino acid mature chemokine. Mouse CXCL7 shares 72% amino acid sequence identity with rat CXCL7. The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract mouse BaF3 cells transfected with human CXCR2, but not human CXCR1.

Bioinformatics

• Gene Symbol: Ppbp
• Uniprot:
  • AAG36786()