Recombinant Mouse Chitinase 3-like 1/YKL-40 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Chitinase 3-like 1/YKL-40 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 10⁴ cells per well are added to rmCHI3-L1 coated plate, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37 °C. The ED₅₀ for this effect is 0.8-3.2 μg/mL.

  Optimal concentrations should be determined by each laboratory for each application.

• Source: Mouse myeloma cell line, NS0-derived mouse Chitinase 3-like 1/YKL-40 protein
  Tyr22-Ala381, with a C-terminal 6-His tag
• Accession #: Q61362
• N-terminal Sequence: Tyr22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Chil1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 41 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Chitinase 3-like 1/YKL-40 Protein, CF

• AW208766
• Brp39
• CHI3L1
• Chitinase 3 like 1
• Chitinase 3-like 1
• gp39
• HCgp39
• YKL-40

Background

Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1 ‑ 4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5 ‑ 7). The mouse CHI3L1 cDNA encodes 381 amino acids (aa), including a 21 aa signal sequence and a 360 aa mature region with two intermolecular disulfides (3). Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin (1 ‑ 4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6 ‑ 9). Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9 ‑ 12). Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15). Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1 (16).

1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
7. Shao, R. et al. (2009) Oncogene 28:4456.
8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
10. Letuve, S. et al. (2008) J Immunol. 181:5167.
11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
16. Lee, C.G. et al. (2009) J. Exp. Med. 206:1149.

Publications for Chitinase 3-like 1/YKL-40 (2649-CH)(4)

Publications using 2649-CH

• Ham, HJ;Lee, YS;Koo, JK;Yun, J;Son, DJ;Han, SB;Hong, JT; Inhibition of Amyloid-? (A?)-Induced Cognitive Impairment and Neuroinflammation in CHI3L1 Knockout Mice through Downregulation of ERK-PTX3 Pathway International journal of molecular sciences 2024-05-19 [PMID: 38791588] (Bioassay, Mouse)
• C Matute-Bla, L Calvo-Barr, I Carballo-C, R Gonzalo, A Sanchez, M Vila, X Montalban, M Comabella Chitinase 3-like 1 is neurotoxic in primary cultured neurons Sci Rep, 2020-04-28;10(1):7118. 2020-04-28 [PMID: 32346016] (Bioassay, Mouse)
• IL-13R?2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses Nat Commun, 2016-09-15;7(0):12752. 2016-09-15 [PMID: 27629921] (Bioassay, Human)
• Tanaka Y, Matsumoto I, Inoue A, Umeda N, Takai C, Sumida T Antigen-specific over-expression of human cartilage glycoprotein 39 on CD4+ CD25+ forkhead box protein 3+ regulatory T cells in the generation of glucose-6-phosphate isomerase-induced arthritis. Clin Exp Immunol, 2014-08-01;177(2):419-27. 2014-08-01 [PMID: 24730590] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Chil1
• Uniprot:
  • Q61362()