Chitinase 3-like 1/YKL-40 Antibodies: Novus Biologicals

AF2599

Chitinase 3-like 1/YKL-40 Antibody [Unconjugated]

100 μg

AF2599

HAF109). A specific band was detected for Chitinase 3-like 1/YKL-40 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of THP-1 human acute monocytic leukemia cell line treated (+) with 50 ng/mL of PMA for 72 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). A specific band was detected for Chitinase 3-like 1/YKL-40 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line treated (+) with 50 ng/mL of PMA for 72 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Chitinase 3-like 1/YKL-40 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

<b>Biological Strategies Validation. </b>Chitinase 3-like 1/YKL-40 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer using Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (<a class=

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>Chitinase 3‑like 1/YKL-40 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line treated with PMA (positive staining) and THP‑1 human acute monocytic leukemia cell line (untreated; negative staining) using Goat Anti-Human Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Chitinase 3-like 1/YKL-40 was detected in immersion fixed paraffin-embedded sections of human cartilage using Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human bone marrow, mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows lysates of Human bone marrow, loaded at 0.2 mg/mL. A specific band was detected for Chitinase 3‑like 1 at approximately 50 kDa (as indicated) using 25 µg/mL of Goat Anti-Human Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 ug/ml LPS for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 50 kDa (as indicated) using 25 µg/mL of Goat Anti-Human Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

YKL-40 expression in AD brain tissue (a) RT-qPCR analysis of YKL-40 in the frontal cortex of control, AD (I-III), AD (IV-VI) and rpAD (IV-VI) samples. GAPDH was used for normalization. Kruskal-Wallis and Dunn’s post-hoc tests were used to estimate statistical differences. b Western blot analysis of YKL-40 in the frontal cortex of control, AD (IV-VI) and rpAD (IV-VI) samples. Normalization was based on GAPDH levels. Graphic summary of densitometry analyses performed on western blot results acquired from 8 control, 8 AD and 6 rpAD samples. c Immunohistochemical analysis of YKL-40 in the cerebral cortex, white matter, subpial layer and cerebellum in control and AD cases. d Immunohistochemical analysis of YKL-40 in the temporal cortex and hippocampus in AD cases. e Immunohistochemical analysis of YKL-40+ astrocytes surrounding beta -amyloid plaques (left) and in blood vessels with amyloid angiopathy (right) in the hippocampal region of AD cases Brown staining corresponds to YKL-40 staining and light blue to haematoxylin counterstaining. f Double-labeling immunofluorescence of YKL-40 (green) and amyloid beta (red) in the hippocampus of AD. g Double-labeling immunofluorescence of YKL-40 (green) and GFAP (red) in cerebral cortex and white matter in AD tissues. Fold changes in expression of mRNA and protein were determined relative to the control cases. *p < 0.05, ***p < 0.001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29126445), licensed under a CC-BY license. Not internally tested by R&D Systems.

YKL-40 expression in DLB brain tissue. a RT-qPCR analysis of YKL-40 in the frontal cortex of control, DLB and rpDLB samples. Normalization was based on GAPDH levels. b Western blot analysis of YKL-40 in the frontal cortex of control, DLB and rpDLB samples. Normalization was carried out with GAPDH. Kruskal-Wallis and Dunn’s post-hoc tests were used for determination of statistical differences. c Immunohistochemical analysis of YKL-40 in the cerebral cortex, white matter and subpial layer in DLB cases. Brown staining corresponds to YKL-40 staining and light blue to haematoxylin counterstaining. Fold changes in expression (mRNA and protein) were determined relative to the control cases Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29126445), licensed under a CC-BY license. Not internally tested by R&D Systems.

YKL-40 expression in the brain tissue of sCJD and related mouse model. a RT-qPCR analysis of YKL-40 in the frontal cortex (left panel) and cerebellum (right panel) of control, sCJD MM1 and sCJD VV2 samples. GAPDH was used for normalization. b Western blot analysis of YKL-40 in the frontal cortex (upper panel) and cerebellum (bottom panel) of control, sCJD MM1 and sCJD VV2 samples. For normalization GAPDH was used. Graphical representation of Western Blot data acquired from the analysis of eight samples per group. Fold changes in the expression of mRNA and protein were determined relative to the control cases. Kruskal-Wallis and Dunn’s post-hoc tests were used to determine statistical differences. ***p < 0.001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29126445), licensed under a CC-BY license. Not internally tested by R&D Systems.

YKL-40 expression in experimental models of prion diseases. a RT-qPCR analysis of YKL-40 in the cortex of control and sCJD MM1 inoculated tg340PRNP129MM mice at 120 dpi (pre-clinical), 160 dpi (early clinical), 180 dpi (clinical) and 210 dpi (clinical with 10–1 diluted inoculum). Four animals per group were analyzed. Normalization was performed using Hprt. b Representative Western-blot analyses for YKL-40 immunodetection in the cortex of control and sCJD MM1-inoculated tg340PRNP129MM mice at 120 dpi (pre-clinical) and 180 dpi (clinical). Three animals per group were analyzed. Normalization was based on beta -actin levels. Numbers indicate densitometry results from three animals per group. Unpaired t-tests were performed to determine statistical differences. c RT-qPCR analysis of YKL-40 in the whole brain of control and RML scrapie-infected mice at pre-clinical (120 dpi) and clinical disease (180 dpi) stages. GAPDH was used for normalization. Similar results were acquired when normalization was based on Hprt expression levels (not shown). Unpaired t-tests were used for estimation of statistical differences. d Immunohistochemical analysis of YKL-40 expression in the cerebral cortex, hippocampus and thalamus of control and RML scrapie-infected mice at pre-clinical (60 and 90 dpi) and clinical (150 dpi) disease stages. Scale bar = 50 μm. Arrows indicate YKL-40 positive reactive astrocytes. Three animals per time point were used. Two sections were stained per animal (sagittal and coronal sections were used). Brown staining corresponds to YKL-40 staining and light blue to haematoxylin counterstaining. e RT-qPCR analysis of YKL-40 in the cerebral cortex of control and 22 L scrapie-infected mice at pre-clinical (60 dpi) and clinical (140 dpi) disease stages. f RT-qPCR analysis of YKL-40 in the cerebellum of control and 22 L scrapie-infected mice at clinical (140 dpi) disease stages. In all cases GAPDH was used for normalization. Similar results were obtained when Hprt was used

2599-CH) was serially diluted and captured by Rat Anti-Human/Primate Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB25991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Chitinase 3‑like 1 (Catalog # <a class=

2599-CH) was serially diluted and captured by Rat Anti-Human/Primate Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB25991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2599U-100UG
Alexa Fluor 405	AF2599V-100UG
Alexa Fluor 488	AF2599G-100UG
Alexa Fluor 532	AF2599X-100UG
Alexa Fluor 594	AF2599T-100UG
Alexa Fluor 647	AF2599R-100UG
Alexa Fluor 700	AF2599N-100UG
Alexa Fluor 750	AF2599S-100UG
Alexa Fluor Plus 405	AF2599AFP405-100UG
Alexa Fluor Plus 488	AF2599AFP488-100UG
Alexa Fluor Plus 555	AF2599AFP555-100UG
Alexa Fluor Plus 594	AF2599AFP594-100UG
Alexa Fluor Plus 647	AF2599AFP647-100UG
Alexa Fluor Plus 680	AF2599AFP680-100UG
Allophycocyanin	AF2599A
Biotin	BAF2599
CoraFluor 1	AF2599CL1
DyLight 350	AF2599D
DyLight 405	AF2599E
DyLight 488	AF2599K
DyLight 550	AF2599L
DyLight 594	AF2599M
DyLight 650	AF2599W
DyLight 680	AF2599Y
DyLight 755	AF2599Z
FITC	AF2599F
HRP	AF2599H
Janelia Fluor 525	AF2599JF525
Janelia Fluor 549	AF2599I
Janelia Fluor 646	AF2599J
Janelia Fluor 669	AF2599JF669
PE	AF2599P
PerCP	AF2599C
mFluor Violet 450 SE	AF2599MFV450
mFluor Violet 500 SE	AF2599MFV500
mFluor Violet 610 SE	AF2599MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2599-SP

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MAB2649

Chitinase 3-like 1/YKL-40 Antibody (321924) [Unconjugated]

(1 Review)

(7 Publications)

100 μg

MAB2649

HAF005). A specific band was detected for Chitinase 3-like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse lung tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB2649) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

HAF005). A specific band was detected for Chitinase 3-like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse lung tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB2649) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Chitinase 3-like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Targeted inhibition of Chi3l1 by dNP2-siChi3l1 complex enhances effector cytokine production in Th1 & CD8 T . a Schematic diagram of the complex formation of dNP2-HA2 peptide & Chi3l1 siRNA (siChi3l1) at indicated N/P ratios. b Gel retardation assay of dNP2-siChi3l1 complex. c The size of free siRNA, free peptide, & dNP2-siRNA complexes measured by Nano particle size analyzer. d Dose dependent reduction of Chi3l1 mRNA expression by dNP2-siChi3l1 complex at 1:25 N/P ratio. e Chi3l1 protein level in culture supernatants measured by ELISA. f Chi3l1 mRNA in the lung after intranasal administration of dNP2-siEGFP or dNP2-siChi3l1 complex at 1:25 N/P ratio. Chi3l1 mRNA normalized to beta -actin, & presented as relative to expression at day 0. g Chi3l1 protein level in the lung was analyzed by WBting. Chi3l1 protein relative to expression at day 0. h Densitometric values of band intensity was calculated by normalization to the value of beta -actin, then the value was represented as relative to the value at day 0. Statistical significance of dNP2-siChi3l1 treated group was analyzed to dNP2-siEGFP treated group on each day. i FACS-sorted WT naïve CD4 T differentiated into Th1 & WT naïve CD8 T activated by plate-bound anti-CD3 & anti-CD28 antibodies with IL-2 for 5 days with indicated concentrations of dNP2-siEGFP or dNP2-siChi3l1 complexes. IFN gamma & TNF alpha expression analyzed by flow cytometry. j % of TNF alpha +IFN gamma + represented as bar graph. k Chi3l1 & IFN gamma mRNA expression in dNP2-siChi3l1 treated Th1 . Each gene expression level was normalized to beta -actin, & represented as fold change to those of WT Th1 cell. Data are presented as mean ± SD of three sets of independent experiments (n = 6). n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed Student’s t-test) Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29403003), licensed under a CC-BY license. Not internally tested by R&D Systems.

Chitinase and Chitinase-like protein expressions in T cells. a, b mRNA expression of chitinase (Chitotriosidase, AMCase) and chitinase-like protein (Chi3l1, Ym-1) in mouse CD4 and CD8 T cells upon anti-CD3 and anti-CD28 stimulation for 3 days. Each gene expression level was normalized to beta -actin, and represented as fold change to Non-activated (NA). c, d Chi3l1 protein level in CD4 and CD8 T cells stimulated by plate-bound anti-CD3 and anti-CD28 antibodies for indicated time point. Densitometric values of band intensity were calculated by normalization to the value of beta -actin. e Chi3l1 mRNA expression in naïve and effector T cell subsets. Chi3l1 mRNA expression level was normalized to beta -actin level. Data are mean ± SD of three sets of independent experiments (n = 6). n.d., not detected; **p < 0.01, ***p < 0.001 (two-tailed Student’s t-test) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29403003), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2649U-100UG
Alexa Fluor 405	FAB2649V-100UG
Alexa Fluor 488	FAB2649G-100UG
Alexa Fluor 532	FAB2649X-100UG
Alexa Fluor 594	FAB2649T-100UG
Alexa Fluor 647	FAB2649R-100UG
Alexa Fluor 700	FAB2649N-100UG
Alexa Fluor 750	FAB2649S-100UG
Alexa Fluor Plus 405	FAB2649AFP405-100UG
Alexa Fluor Plus 488	FAB2649AFP488-100UG
Alexa Fluor Plus 555	FAB2649AFP555-100UG
Alexa Fluor Plus 594	FAB2649AFP594-100UG
Alexa Fluor Plus 647	FAB2649AFP647-100UG
Alexa Fluor Plus 680	FAB2649AFP680-100UG
Allophycocyanin	FAB2649A
Biotin	FAB2649B
CoraFluor 1	FAB2649CL1
DyLight 350	FAB2649D
DyLight 405	FAB2649E
DyLight 488	FAB2649K
DyLight 550	FAB2649L
DyLight 594	FAB2649M
DyLight 650	FAB2649W
DyLight 680	FAB2649Y
DyLight 755	FAB2649Z
FITC	FAB2649F
HRP	FAB2649H
Janelia Fluor 525	FAB2649JF525
Janelia Fluor 549	FAB2649I
Janelia Fluor 646	FAB2649J
Janelia Fluor 669	FAB2649JF669
PE	FAB2649P
PerCP	FAB2649C
mFluor Violet 450 SE	FAB2649MFV450
mFluor Violet 500 SE	FAB2649MFV500
mFluor Violet 610 SE	FAB2649MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2649-SP

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NBP3-21212

Chitinase 3-like 1/YKL-40 Antibody (CL12241) - BSA Free

100 ul

NBP3-21212

Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.

Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in glial cells.

Staining of human astrocytoma shows strong cytoplasmic positivity in tumor cells.

Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human glioblastoma shows moderate to strong cytoplasmic positivity in tumor cells.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44298AF350
Alexa Fluor 405	NBP3-44298AF405
Alexa Fluor 488	NBP3-44298AF488
Alexa Fluor 532	NBP3-44298AF532
Alexa Fluor 594	NBP3-44298AF594
Alexa Fluor 647	NBP3-44298AF647
Alexa Fluor 700	NBP3-44298AF700
Alexa Fluor 750	NBP3-44298AF750
Alexa Fluor Plus 405	NBP3-44298AFP405
Alexa Fluor Plus 488	NBP3-44298AFP488
Alexa Fluor Plus 555	NBP3-44298AFP555
Alexa Fluor Plus 594	NBP3-44298AFP594
Alexa Fluor Plus 647	NBP3-44298AFP647
Alexa Fluor Plus 680	NBP3-44298AFP680
Allophycocyanin	NBP3-44298APC
Biotin	NBP3-44298B
CoraFluor 1	NBP3-44298CL1
DyLight 350	NBP3-44298UV
DyLight 405	NBP3-44298V
DyLight 488	NBP3-44298G
DyLight 550	NBP3-44298R
DyLight 594	NBP3-44298DL594
DyLight 650	NBP3-44298C
DyLight 680	NBP3-44298FR
DyLight 755	NBP3-44298IR
FITC	NBP3-44298F
HRP	NBP3-44298H
Janelia Fluor 525	NBP3-44298JF525
Janelia Fluor 549	NBP3-44298JF549
Janelia Fluor 646	NBP3-44298JF646
Janelia Fluor 669	NBP3-44298JF669
PE	NBP3-44298PE
PerCP	NBP3-44298PCP
mFluor Violet 450 SE	NBP3-44298MFV450
mFluor Violet 500 SE	NBP3-44298MFV500
mFluor Violet 610 SE	NBP3-44298MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21212-25ul
Azide and BSA Free	NBP3-44298

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AF2649

Chitinase 3-like 1/YKL-40 Antibody [Unconjugated]

(3 Publications)

100 μg

AF2649

HAF016). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, THP‑1 human acute monocytic leukemia cell line + PMA/LPS untreated (-) or treated (+) with 200 nm, 10 μg/ml PMA and LPS for 24hr, 3hr. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2649) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, THP‑1 human acute monocytic leukemia cell line + PMA/LPS untreated (-) or treated (+) with 200 nm, 10 μg/ml PMA and LPS for 24hr, 3hr. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2649) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF016). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2649) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows lysates of Mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 50 kDa (as indicated) using 25 µg/mL of Sheep Anti-Mouse Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2649) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunodepletion of CHIL1 in A-Neu supernatant attenuated the inhibitory effect on pro-inflammatory macrophages. (A) Schematic representation of the experimental design: bone marrow-derived macrophages were stimulated with LPS 10 ng/mL for 3 hours, washed with PBS, and then cultured in the presence of A-Neu supernatant (SUP) or A-Neu supernatant immunodepleted with anti-CHIL1 (SUP-anti-CHIL1). Expressions of CCL4, TNF and NOS2 in indicated groups were detected by RT-qPCR (B, n=6), CBA (C, D, n=4) and WB (E, n=3). The results were presented as mean ± SD. One-way ANOVA was used. *P < 0.05. A-Neu, activated neutrophil; N-Neu, non-activated neutrophil; SUP, activated neutrophil supernatant; SUP-anti-CHIL1, activated neutrophil supernatant immunodepletion CHIL1; Ccl4, C-C chemokine motif ligand 4; Tnf, tumor necrosis factor; Nos2, nitric oxide synthase 2; LPS, lipopolysaccharide. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35529851), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2649U-100UG
Alexa Fluor 405	AF2649V-100UG
Alexa Fluor 488	AF2649G-100UG
Alexa Fluor 532	AF2649X-100UG
Alexa Fluor 594	AF2649T-100UG
Alexa Fluor 647	AF2649R-100UG
Alexa Fluor 700	AF2649N-100UG
Alexa Fluor 750	AF2649S-100UG
Alexa Fluor Plus 405	AF2649AFP405-100UG
Alexa Fluor Plus 488	AF2649AFP488-100UG
Alexa Fluor Plus 555	AF2649AFP555-100UG
Alexa Fluor Plus 594	AF2649AFP594-100UG
Alexa Fluor Plus 647	AF2649AFP647-100UG
Alexa Fluor Plus 680	AF2649AFP680-100UG
Biotin	BAF2649

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2649-SP

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MAB25991

Chitinase 3-like 1/YKL-40 Antibody (384327) [Unconjugated]

(3 Publications)

100 μg

MAB25991

CTLA4-related gene expression in TN-BCSCs. A Expression heatmap of the DEGs between TN-BCSCs and B.C.s (CD44− and CD24+). The red indicates the upregulated expression, and the green indicates the downregulated expression. B Intersection between DEGs in TN-BCSC and CTLA4-related genes (CTLA4 Gene Term). C Expression distribution plot of CTLA4-related DEGs in TN-BCSCs in eight cell subsets. D Violin plots of FBXO9, CHI3L1 and MYC. E The correlation between CHI3L1 and CTLA4 expression in B.C. was obtained from the TIMER2.0 database. The result is corrected by tumor purity. F and G The CHI3L1 expression in BCSCs obtained from MDA-MB-231 and MDA-MB-453 cells and the parental cells of BCSCs as measured by RT-qPCR and Western blot. H The expression of CHI3L1 in the supernatant of conditioned medium of BCSCs obtained from MDA-MB-231 and MDA-MB-453 cells and the parental cells of BCSCs as determined by ELISA. nsp > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. Cell experiments were repeated three times Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37838657), licensed under a CC-BY license. Not internally tested by R&D Systems.

2599-CH) was serially diluted and captured by Rat Anti-Human/Primate Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB25991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

2599-CH) was serially diluted and captured by Rat Anti-Human/Primate Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB25991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB25991U-100UG
Alexa Fluor 405	FAB25991V-100UG
Alexa Fluor 488	FAB25991G-100UG
Alexa Fluor 532	FAB25991X-100UG
Alexa Fluor 594	FAB25991T-100UG
Alexa Fluor 647	FAB25991R-100UG
Alexa Fluor 700	FAB25991N-100UG
Alexa Fluor 750	FAB25991S-100UG
Alexa Fluor Plus 405	FAB25991AFP405-100UG
Alexa Fluor Plus 488	FAB25991AFP488-100UG
Alexa Fluor Plus 555	FAB25991AFP555-100UG
Alexa Fluor Plus 594	FAB25991AFP594-100UG
Alexa Fluor Plus 647	FAB25991AFP647-100UG
Alexa Fluor Plus 680	FAB25991AFP680-100UG
Allophycocyanin	FAB25991A
Biotin	FAB25991B
CoraFluor 1	FAB25991CL1
DyLight 350	FAB25991D
DyLight 405	FAB25991E
DyLight 488	FAB25991K
DyLight 550	FAB25991L
DyLight 594	FAB25991M
DyLight 650	FAB25991W
DyLight 680	FAB25991Y
DyLight 755	FAB25991Z
FITC	FAB25991F
HRP	FAB25991H
Janelia Fluor 525	FAB25991JF525
Janelia Fluor 549	FAB25991I
Janelia Fluor 646	FAB25991J
Janelia Fluor 669	FAB25991JF669
PE	FAB25991P
PerCP	FAB25991C
mFluor Violet 450 SE	FAB25991MFV450
mFluor Violet 500 SE	FAB25991MFV500
mFluor Violet 610 SE	FAB25991MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB25991-SP
Carrier Free	MAB25991-500

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NBP3-15132

Chitinase 3-like 1/YKL-40 Antibody (CL12314) - BSA Free

100 ul

NBP3-15132

Immunohistochemistry-Paraffin: Chitinase 3-like 1 Antibody (CL12314) [NBP3-15132] - Staining of human astrocytoma shows strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: Chitinase 3-like 1 Antibody (CL12314) [NBP3-15132] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in glial cells.

Immunohistochemistry-Paraffin: Chitinase 3-like 1 Antibody (CL12314) [NBP3-15132] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Chitinase 3-like 1 Antibody (CL12314) [NBP3-15132] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44361AF350
Alexa Fluor 405	NBP3-44361AF405
Alexa Fluor 488	NBP3-44361AF488
Alexa Fluor 532	NBP3-44361AF532
Alexa Fluor 594	NBP3-44361AF594
Alexa Fluor 647	NBP3-44361AF647
Alexa Fluor 700	NBP3-44361AF700
Alexa Fluor 750	NBP3-44361AF750
Alexa Fluor Plus 405	NBP3-44361AFP405
Alexa Fluor Plus 488	NBP3-44361AFP488
Alexa Fluor Plus 555	NBP3-44361AFP555
Alexa Fluor Plus 594	NBP3-44361AFP594
Alexa Fluor Plus 647	NBP3-44361AFP647
Alexa Fluor Plus 680	NBP3-44361AFP680
Allophycocyanin	NBP3-44361APC
Biotin	NBP3-44361B
CoraFluor 1	NBP3-44361CL1
DyLight 350	NBP3-44361UV
DyLight 405	NBP3-44361V
DyLight 488	NBP3-44361G
DyLight 550	NBP3-44361R
DyLight 594	NBP3-44361DL594
DyLight 650	NBP3-44361C
DyLight 680	NBP3-44361FR
DyLight 755	NBP3-44361IR
FITC	NBP3-44361F
HRP	NBP3-44361H
Janelia Fluor 525	NBP3-44361JF525
Janelia Fluor 549	NBP3-44361JF549
Janelia Fluor 646	NBP3-44361JF646
Janelia Fluor 669	NBP3-44361JF669
PE	NBP3-44361PE
PerCP	NBP3-44361PCP
mFluor Violet 450 SE	NBP3-44361MFV450
mFluor Violet 500 SE	NBP3-44361MFV500
mFluor Violet 610 SE	NBP3-44361MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-15132-25ul
Azide and BSA Free	NBP3-44361

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NBP3-28760

Chitinase 3-like 1/YKL-40 Antibody (anti-CHI3L1) - Chimeric - Low Endotoxin, Azide and BSA Free

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28760AF350
Alexa Fluor 405	NBP3-28760AF405
Alexa Fluor 488	NBP3-28760AF488
Alexa Fluor 532	NBP3-28760AF532
Alexa Fluor 594	NBP3-28760AF594
Alexa Fluor 647	NBP3-28760AF647
Alexa Fluor 700	NBP3-28760AF700
Alexa Fluor 750	NBP3-28760AF750
Alexa Fluor Plus 405	NBP3-28760AFP405
Alexa Fluor Plus 488	NBP3-28760AFP488
Alexa Fluor Plus 555	NBP3-28760AFP555
Alexa Fluor Plus 594	NBP3-28760AFP594
Alexa Fluor Plus 647	NBP3-28760AFP647
Alexa Fluor Plus 680	NBP3-28760AFP680
Allophycocyanin	NBP3-28760APC
Biotin	NBP3-28760B
CoraFluor 1	NBP3-28760CL1
DyLight 350	NBP3-28760UV
DyLight 405	NBP3-28760V
DyLight 488	NBP3-28760G
DyLight 550	NBP3-28760R
DyLight 594	NBP3-28760DL594
DyLight 650	NBP3-28760C
DyLight 680	NBP3-28760FR
DyLight 755	NBP3-28760IR
FITC	NBP3-28760F
HRP	NBP3-28760H
Janelia Fluor 525	NBP3-28760JF525
Janelia Fluor 549	NBP3-28760JF549
Janelia Fluor 646	NBP3-28760JF646
Janelia Fluor 669	NBP3-28760JF669
PE	NBP3-28760PE
PerCP	NBP3-28760PCP
mFluor Violet 450 SE	NBP3-28760MFV450
mFluor Violet 500 SE	NBP3-28760MFV500
mFluor Violet 610 SE	NBP3-28760MFV610

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NBP3-16077

Chitinase 3-like 1/YKL-40 Antibody (7T1R3)

100 ul

NBP3-16077

Immunocytochemistry/ Immunofluorescence: Chitinase 3-like 1/YKL-40 Antibody (7T1R3) [NBP3-16077] - Confocal imaging of THP-1 cells using Chitinase 3-like 1/YKL-40 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunocytochemistry/ Immunofluorescence: Chitinase 3-like 1/YKL-40 Antibody (7T1R3) [NBP3-16077] - Confocal imaging of paraffin-embedded Rat brain tissue using Chitinase 3-like 1/YKL-40 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-57913

Chitinase 3-like 1/YKL-40 Antibody - BSA Free

(1 Publication)

100 ul

NBP1-57913

Reactivity:

Hu, Pm, V-Vi

Species Glossary

Reactivity: Hu, Pm, V-Vi

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-21409

Chitinase 3-like 1/YKL-40 Antibody - BSA Free

100 ul

NBP3-21409

Staining of human cell line ASC TERT1 shows localization to the Golgi apparatus.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21409-25ul

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NBP3-41449

Chitinase 3-like 1/YKL-40 Antibody (C4) - BSA Free

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-48367

Chitinase 3-like 1/YKL-40 Antibody (DMC483) - Chimeric

100 μg

NBP3-48367

Flow cytometry analysis with Anti-Chitinase 3-like 1/YKL-40 (DMC483) on HEK293 cells transfected with human Chitinase 3-like 1/YKL-40 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Chitinase 3-like 1/YKL-40 Antibody [Unconjugated]

Chitinase 3-like 1/YKL-40 Antibody (321924) [Unconjugated]

Chitinase 3-like 1/YKL-40 Antibody (CL12241) - BSA Free

Chitinase 3-like 1/YKL-40 Antibody [Unconjugated]

Chitinase 3-like 1/YKL-40 Antibody (384327) [Unconjugated]

Chitinase 3-like 1/YKL-40 Antibody (CL12314) - BSA Free

Chitinase 3-like 1/YKL-40 Antibody (anti-CHI3L1) - Chimeric - Low Endotoxin, Azide and BSA Free

Chitinase 3-like 1/YKL-40 Antibody (7T1R3)

Chitinase 3-like 1/YKL-40 Antibody - BSA Free

Chitinase 3-like 1/YKL-40 Antibody - BSA Free

Chitinase 3-like 1/YKL-40 Antibody (C4) - BSA Free

Chitinase 3-like 1/YKL-40 Antibody (DMC483) - Chimeric