YM1/Chitinase 3-like 3 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3‑like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by ...read more
Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3‑like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

YM1/Chitinase 3-like 3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like 3
Tyr22-Tyr398
Accession # O35744
Specificity
Detects mouse YM1/Chitinase 3-like 3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHI3L2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 2.5 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF2446 in the following applications:

Publications
Read Publications using
AF2446 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for YM1/Chitinase 3-like 3 Antibody [Unconjugated]

  • AI505981
  • CHI3L3
  • Chitinase 3 like 3
  • Chitinase 3-like 3
  • ECF-L
  • YM1

Background

YM1, also known as Chitinase 3-like 3 and ECF-L, is a secreted 377 amino acid residue lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. YM1 shares homology with chitinase family proteins but lacks enzymatic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for YM1/Chitinase 3-like 3 Antibody (AF2446)(14)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC
(5)
IHC-Fr
(1)
IHC-P
(3)
Western Blot
(4)
All Applications
Filter By Species
Mouse
(14)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF2446 Applications Species
D Cerezo-Wal, M Contreras-, K Troulé, X Catena, C Mucientes, TG Calvo, E Cañón, C Tejedo, PC Pennacchi, S Hogan, P Kölblinger, H Tejero, AX Chen, N Ibarz, O Graña-Cast, L Martinez, J Muñoz, P Ortiz-Rome, JL Rodriguez-, G Gómez-Lópe, F Al-Shahrou, R Rabadán, MP Levesque, D Olmeda, MS Soengas Midkine rewires the melanoma microenvironment toward a tolerogenic and immune-resistant state Nat Med, 2020;0(0):. 2020 [PMID: 33077955] (IHC, Mouse) IHC Mouse
AL Chenery, R Alhallaf, Z Agha, J Ajendra, JE Parkinson, MM Cooper, BHK Chan, RM Eichenberg, LA Dent, AAB Robertson, A Kupz, D Brough, A Loukas, TE Sutherland, JE Allen, PR Giacomin Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung J. Immunol., 2019;203(10):2724-2734. 2019 [PMID: 31586037] (Flow Cytometry, Mouse) Flow Cytometry Mouse
LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism Mucosal Immunol, 2018;0(0):. 2018 [PMID: 30337651] (IHC, Mouse) IHC Mouse
H Lu, S Chelvanamb, C Poirier, J Saliba, KL March, M Clauss, NV Bogatcheva EMAPII Monoclonal Antibody Ameliorates Influenza A Virus-Induced Lung Injury Mol. Ther., 2018;0(0):. 2018 [PMID: 29910176] (Western Blot, Mouse) Western Blot Mouse
C Draijer, P Robbe, CE Boorsma, MN Hylkema, BN Melgert Dual role of YM1+ M2 macrophages in allergic lung inflammation Sci Rep, 2018;8(1):5105. 2018 [PMID: 29572536] (IHC-P, Mouse) IHC-P Mouse
J Dong, Q Ma Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis Nanotoxicology, 2018;0(0):1-16. 2018 [PMID: 29338488] (Western Blot, Mouse) Western Blot Mouse
TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158. 2016 [PMID: 27911817] (Western Blot, Mouse) Western Blot Mouse
Laurie P Shornick Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20 Adv Wound Care (New Rochelle), 2016;5(11):486-494. 2016 [PMID: 27867753] (IHC, Mouse) IHC Mouse
Severe Reduction in Number and Function of Peripheral T Cells Does Not Afford Protection toward Emphysema and Bronchial Remodeling Induced in Mice by Cigarette Smoke Am J Pathol, 2016;0(0):. 2016 [PMID: 27157991] (IHC-P, Mouse) IHC-P Mouse
Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema. PLoS ONE, 2015;10(3):e0119797. 2015 [PMID: 25790295] (IHC, Mouse) IHC Mouse
Show All 14 Publications.

Review for YM1/Chitinase 3-like 3 Antibody (AF2446) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2446:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen YM1/Chitinase 3-like 3 AF2446
Enlarge
5
reviewed by:
Branislav Krljanac
IHC-Fr Mouse 07/16/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedNippostrongylus brasiliensis infected mouse lung (day 14 post infection)
SpeciesMouse
LotUNU0115051

Comments

CommentsNippostrongylus brasiliensis infected mouse lung (day 14 post infection)
Frozen section (acetone fixed)
Blocking in 2,5% donkey serum (30min)
Goat-Ym-1 (AF2446) 1/100 in PBS+2,5% BSA (1h)
Donkey-anti-Goat-A488 1/150 in 2,5% donkey serum (1h)
Mounting media containing DAPI.

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FAQs for YM1/Chitinase 3-like 3 Antibody (AF2446) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2446

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Diseases for YM1/Chitinase 3-like 3 Antibody (AF2446)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Branislav Krljanac
07/16/2017
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CHI3L2
Uniprot