Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF

Recombinant Mouse CD36/SR‑B3 Fc Chimera (Catalog # 2519-CD) is used at 1 µg/mL, Recombinant Human Thrombospondin-2 (Catalog # 1635-T2) binds with an ED50 of 0.06‑0.6 μg/mL.

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. In a Goat anti-Human IgG Fc antibody (Catalog # G-102-C) coated plate, Recombinant Mouse CD36/SR‑B3 Fc Chimera using at 1 µg/mL binds Recombinant Human Thrombospondin-2 (Catalog # 1635-T2) with an ED₅₀ of 0.06-0.6 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse CD36/SR-B3 protein
  

  Mouse CD36 (Gly30-Lys439) Accession # Q3UAI3	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q3UAI3
• N-terminal Sequence: Gly30
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd36
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 72.9 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-130 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF

• BDPLT10
• CD_antigen: CD36
• CD36 antigen (collagen type I receptor, thrombospondin receptor)
• CD36 antigen
• CD36 molecule (thrombospondin receptor)
• CD36
• CHDS7
• cluster determinant 36
• Collagen R
• FAT
• Fatty acid translocase
• Glycoprotein IIIb
• GP3B
• GP4
• GPIIIb
• GPIV
• PAS IV
• PAS-4 protein
• PAS-4
• PASIV
• Platelet collagen receptor
• platelet glycoprotein 4
• SCARB3
• scavenger receptor class B, member 3
• SRB3
• SR-B3
• Thrombospondin R
• Thrombospondin receptor

Background

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N-terminal cytoplasmic domain, a 22 aa N-terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C-terminal transmembrane segment, and a 9 aa C-terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C-terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N-terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84% and 84% aa identical to the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 
2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 
3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 
4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 
5. Malaud, E. et al. (2002) Biochem. J. 364:507.
6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.

Publications for CD36/SR-B3 (2519-CD)(3)

Publications using 2519-CD

• Alvarez, JA;Gas-Pascual, E;Malhi, S;Sánchez-Arcila, JC;Njume, FN;van der Wel, H;Zhao, Y;García-López, L;Ceron, G;Posada, J;Souza, SP;Yap, GS;West, CM;Jensen, KDC; The GPI sidechain of Toxoplasma gondii inhibits parasite pathogenesis mBio 2024-09-20 [PMID: 39302131] (Bioassay, T. gondii)
• S Silva Pere, M De Niz, K Serre, M Ouarné, JE Coelho, CA Franco, LM Figueiredo Immunopathology and Trypanosoma congolense parasite sequestration cause acute cerebral trypanosomiasis Elife, 2022-07-05;11(0):. 2022-07-05 [PMID: 35787830] (Bioassay, Mouse)
• Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages. PLoS ONE, 2015-04-07;10(4):e0123293. 2015-04-07 [PMID: 25849867] (Binding Assay, Mouse)

Bioinformatics

• Gene Symbol: Cd36
• Uniprot:
  • Q3UAI3()