Recombinant Mouse CD163 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD163 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD163 is immobilized at 5 μg/mL (100 μL/well), the concentration of mouse Haptoglobin/Hemoglobin (Hp/Hb) that produces 50% optimal binding response is approximately 0.2-1 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CD163 protein
  Val39-Thr1045, with a C-terminal 10-His tag
• Accession #: AAK16065
• N-terminal Sequence: Val39
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd163
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-160 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD163 Protein, CF

• CD_antigen: CD163
• CD163 molecule
• CD163
• GHI/61
• HbSR
• Hemoglobin scavenger receptor
• M130
• macrophage-associated antigen
• MM130
• RM3/1
• SCARI1
• scavenger receptor cysteine-rich type 1 protein M130
• sCD163
• Soluble CD163

Background

CD163, also known as M130 and  p155, is a 130-160 kDa type I transmembrane protein belonging to group B of the cysteine-rich scavenger receptor family (1). Mature mouse CD163 consists of a 1007 amino acid (aa) extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (2). Alternative splicing of mouse CD163 generates an isoform with a substitution in the cytoplasmic region. Within the ECD, mouse CD163 shares 75% and 90% aa sequence identity with human and rat CD163, respectively. CD163 is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10 (3-5). A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage (6, 7) in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6 (8-10). CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines (11). It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin (12, 13). It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion (14). CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis (15, 16).

1. Kowal, K. et al. (2011) Folia Histochem. Cytobiol. 49:365.
2. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
3. Hogger, P. et al. (1998) J. Immunol. 161:1883.
4. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
5. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
6. Etzerodt, A. et al. (2010) J. Leukoc. Biol. 88:1201.
7. Moreno, J.A. et al. (2012) Eur. Heart J. 33:252.
8. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
9. Timmermann, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
10. Sulahian, T.H. et al. (2004) J. Leukoc. Biol. 76:271.
11. Fabriek, B.O. et al. (2009) Blood 113:887.
12. Kristiansen, M. et al. (2001) Nature 409:198.
13. Schaer, D.J. et al. (2006) Blood 107:373.
14. Fabriek, B.O. et al. (2007) Blood 109:5223.
15. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
16. Moreno, J.A. et al. (2009) Atherosclerosis 207:103.

Publications for CD163 (7435-CD)(2)

Publications using 7435-CD

• Chen, L;Mei, W;Song, J;Chen, K;Ni, W;Wang, L;Li, Z;Ge, X;Su, L;Jiang, C;Liu, B;Dai, C; CD163 protein inhibits lipopolysaccharide-induced macrophage transformation from M2 to M1 involved in disruption of the TWEAK-Fn14 interaction Heliyon 2024-01-15 [PMID: 38148798] (Bioassay, Mouse)
• P Scodeller, L Simón-Grac, S Kopanchuk, A Tobi, K Kilk, P Säälik, K Kurm, ML Squadrito, VR Kotamraju, A Rinken, M De Palma, E Ruoslahti, T Teesalu Precision Targeting of Tumor Macrophages with a CD206 Binding Peptide Sci Rep, 2017-11-07;7(1):14655. 2017-11-07 [PMID: 29116108] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Cd163
• Uniprot:
  • AAK16065()