Recombinant Mouse CCL25/TECK Protein, CF


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Recombinant Mouse CCL25/TECK Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR9. The ED50 for this effect is 0.1-0.5 µg/mL.
E. coli-derived mouse CCL25/TECK protein
Gln24-Asn144, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
16-17 kDa doublet, reducing conditions
Read Publications using
481-TK/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL25/TECK Protein, CF

  • C-C motif chemokine 25
  • CCL25
  • chemokine (C-C motif) ligand 25
  • Chemokine TECK
  • Ck beta-15
  • Ckb15
  • SCYA25TECKvar
  • small inducible cytokine subfamily A (Cys-Cys), member 25
  • Small-inducible cytokine A25
  • TECK
  • TECKMGC150327
  • thymus expressed chemokine
  • Thymus-expressed chemokine


CCL25, also known as TECK (thymus-expressed chemokine), is a CC chemokine that regulates the trafficking of lymphocytes in the thymus and small intestine. Mature mouse CCL25 shares 40% and 81% amino acid sequence identity with human and rat CCL25, respectively (1). CCL25 is produced by stromal cells in the thymus and epithelial cells of the small intestine, particularly the jejunum and ileum (1-3). It binds to and induces chemoattraction through CCR9 (1, 4, 5), and both human and mouse proteins act on human CCR9 (4). CCR9 is expressed on immature pre-T cells and thymocytes (5, 6). CCL25 induces the homing of several lymphocyte populations to the small intestine (3), including Integrin alpha 4 beta 7+ gamma δ T cells (6, 7), Integrin alpha E beta 7+ CD8+ T cells (8), and IgA-producing plasma cells (2). In cancer, functional CCR9 mediates the metastasis of melanoma cells to the small intestine (9), contributes to the CCL25-dependent migration and invasion of some breast carcinomas (10), and attracts mesenchymal stromal cells to CCL25-expressing multiple myelomas (11). CCL25 contributes to the severity of chronic inflammation in rheumatoid arthritis where it attracts CCR9+ monocytes and macrophages (12), in endometriosis where it promotes the invasiveness of stromal cells (13), and in atherosclerosis where it contributes to the accumulation of CCR9+ macrophages in arterial plaques (14).
  1. Vicari, A.P. et al. (1997) Immunity 7:291.
  2. Bowman, E.P. et al. (2002) J. Exp. Med. 195:269.
  3. Kunkel, E.J. et al. (2000) J. Exp. Med. 192:761.
  4. Zaballos, A. et al. (1999) J. Immunol. 162:5671.
  5. Youn, B.-S. et al. (1999) Blood 94:2533.
  6. Uehara, S. et al. (2002) J. Immunol. 168:134.
  7. Costa, M.F.S. et al. (2012) Eur. J. Immunol. 42:1250.
  8. Svensson, M. et al. (2002) J. Clin. Invest. 110:1113.
  9. Amersi, F.F. et al. (2008) Clin. Cancer Res. 14:638.
  10. Johnson-Holiday, C. et al. (2011) Int. J. Oncol. 38:1279.
  11. Xu, S. et al. (2012) Stem Cells 30:266.
  12. Schmutz, C. et al. (2010) Arthritis Res. Ther. 12:R161.
  13. Wang, Y. et al. (2010) Cell. Mol. Immunol. 7:51.
  14. Alla, J.A. et al. (2010) J. Biol. Chem. 285:23496.

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Publications for CCL25/TECK (481-TK/CF)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 481-TK/CF Applications Species
C Li, HK Kim, P Prakhar, S Luo, A Crossman, DL Ligons, MA Luckey, P Awasthi, RE Gress, JH Park Chemokine receptor CCR9 suppresses the differentiation of CD4+CD8alphaalpha+ intraepithelial T cells in the gut Mucosal Immunology, 2022-07-01;0(0):. 2022-07-01 [PMID: 35778600] (Bioassay, Mouse) Bioassay Mouse
V Tereshchen, A Bulygin, R Zavodskii, A Maksyutov, V Kurilin, M Fisher, N Semenyuk, S Aladev, S Sennikov The murine DCs transfected with DNA-plasmid encoding CCR9 demonstrate the increased migration to CCL25 and thymic cells in vitro and to the thymus in vivo Cytokine, 2021-02-26;142(0):155473. 2021-02-26 [PMID: 33647585] (Bioassay, Mouse) Bioassay Mouse
H Zhang, Y Zheng, Y Pan, C Lin, S Wang, Z Yan, L Lu, G Ge, J Li, YA Zeng, J Chen A mutation that blocks integrin alpha4beta7 activation prevents adaptive immune-mediated colitis without increasing susceptibility to innate colitis BMC Biol., 2020-06-10;18(1):64. 2020-06-10 [PMID: 32522281] (Bioassay, Mouse) Bioassay Mouse
SM Pontejo, C Sanchez, B Ruiz-Argüe, A Alcami Insights into ligand binding by a viral tumor necrosis factor (TNF) decoy receptor yield a selective soluble human type 2 TNF receptor J. Biol. Chem., 2019-02-05;0(0):. 2019-02-05 [PMID: 30723161] (Bioassay, Mouse) Bioassay Mouse
FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus Elife, 2018-09-06;7(0):. 2018-09-06 [PMID: 30187863] (Bioassay, Mouse) Bioassay Mouse
S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 2017-04-10;14(5):531-538. 2017-04-10 [PMID: 28394335] (Bioassay, Mouse) Bioassay Mouse
Chen H, Sun J, Huang Z, Hou H, Arcilla M, Rakhilin N, Joe D, Choi J, Gadamsetty P, Milsom J, Nandakumar G, Longman R, Zhou X, Edwards R, Chen J, Chen K, Bu P, Wang L, Xu Y, Munroe R, Abratte C, Miller A, Gumus Z, Shuler M, Nishimura N, Edelmann W, Shen X, Lipkin S Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting. Nat Biotechnol, 2015-05-25;33(6):656-60. 2015-05-25 [PMID: 26006007] (Bioassay, Human) Bioassay Human
Graham K, Zhang J, Lewen S, Burke T, Dang T, Zoudilova M, Sobel R, Butcher E, Zabel B A novel CMKLR1 small molecule antagonist suppresses CNS autoimmune inflammatory disease. PLoS ONE, 2014-12-01;9(12):e112925. 2014-12-01 [PMID: 25437209] (Bioassay, Mouse) Bioassay Mouse
Miething , Corneliu, Scuoppo , Claudio, Bosbach , Benedikt, Appelmann , Iris, Nakitandwe , Joy, Ma , Jing, Wu , Gang, Lintault , Laura, Auer , Martina, Premsrirut , Prem K, Teruya-Feldstein , Julie, Hicks , James, Benveniste , Helene, Speicher , Michael, Downing , James R, Lowe , Scott W PTEN action in leukaemia dictated by the tissue microenvironment. Nature, 2014-05-04;510(7505):402-6. 2014-05-04 [PMID: 24805236] (Bioassay, Mouse) Bioassay Mouse
Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction. Eur. J. Immunol., 2012-05-01;42(5):1250-60. 2012-05-01 [PMID: 22539297] (In Vivo, Mouse) In Vivo Mouse
Show All 16 Publications.

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Gene Symbol Ccl25