>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Theoretical MW
14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
16-17 kDa doublet, reducing conditions
Publications
Read Publications using 481-TK/CF in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse CCL25/TECK Protein, CF
C-C motif chemokine 25
CCL25
chemokine (C-C motif) ligand 25
Chemokine TECK
Ck beta-15
Ckb15
SCYA25TECKvar
small inducible cytokine subfamily A (Cys-Cys), member 25
Small-inducible cytokine A25
TECK
TECKMGC150327
thymus expressed chemokine
Thymus-expressed chemokine
Background
CCL25, also known as TECK (thymus-expressed chemokine), is a CC chemokine that regulates the trafficking of lymphocytes in the thymus and small intestine. Mature mouse CCL25 shares 40% and 81% amino acid sequence identity with human and rat CCL25, respectively (1). CCL25 is produced by stromal cells in the thymus and epithelial cells of the small intestine, particularly the jejunum and ileum (1-3). It binds to and induces chemoattraction through CCR9 (1, 4, 5), and both human and mouse proteins act on human CCR9 (4). CCR9 is expressed on immature pre-T cells and thymocytes (5, 6). CCL25 induces the homing of several lymphocyte populations to the small intestine (3), including Integrin alpha 4 beta 7+ gamma δ T cells (6, 7), Integrin alpha E beta 7+ CD8+ T cells (8), and IgA-producing plasma cells (2). In cancer, functional CCR9 mediates the metastasis of melanoma cells to the small intestine (9), contributes to the CCL25-dependent migration and invasion of some breast carcinomas (10), and attracts mesenchymal stromal cells to CCL25-expressing multiple myelomas (11). CCL25 contributes to the severity of chronic inflammation in rheumatoid arthritis where it attracts CCR9+ monocytes and macrophages (12), in endometriosis where it promotes the invasiveness of stromal cells (13), and in atherosclerosis where it contributes to the accumulation of CCR9+ macrophages in arterial plaques (14).
Vicari, A.P. et al. (1997) Immunity 7:291.
Bowman, E.P. et al. (2002) J. Exp. Med. 195:269.
Kunkel, E.J. et al. (2000) J. Exp. Med. 192:761.
Zaballos, A. et al. (1999) J. Immunol. 162:5671.
Youn, B.-S. et al. (1999) Blood 94:2533.
Uehara, S. et al. (2002) J. Immunol. 168:134.
Costa, M.F.S. et al. (2012) Eur. J. Immunol. 42:1250.
Svensson, M. et al. (2002) J. Clin. Invest. 110:1113.
Amersi, F.F. et al. (2008) Clin. Cancer Res. 14:638.
Johnson-Holiday, C. et al. (2011) Int. J. Oncol. 38:1279.
Xu, S. et al. (2012) Stem Cells 30:266.
Schmutz, C. et al. (2010) Arthritis Res. Ther. 12:R161.
Wang, Y. et al. (2010) Cell. Mol. Immunol. 7:51.
Alla, J.A. et al. (2010) J. Biol. Chem. 285:23496.
Discover related pathways, diseases and genes to CCL25/TECK (481-TK/CF). Need help?
Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for CCL25/TECK (481-TK/CF)
Discover more about diseases related to CCL25/TECK (481-TK/CF).
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our Recombinant Mouse CCL25/TECK Protein, CF and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.