Recombinant Mouse CCL25/TECK Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CCL25/TECK Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR9. The ED₅₀ for this effect is 0.1-0.5 µg/mL.
• Source: E. coli-derived mouse CCL25/TECK protein
  Gln24-Asn144, with an N-terminal Met
• Accession #: O35903.1
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ccl25
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 16-17 kDa doublet, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL25/TECK Protein, CF

• C-C motif chemokine 25
• CCL25
• chemokine (C-C motif) ligand 25
• Chemokine TECK
• Ck beta-15
• Ckb15
• SCYA25TECKvar
• small inducible cytokine subfamily A (Cys-Cys), member 25
• Small-inducible cytokine A25
• TECK
• TECKMGC150327
• thymus expressed chemokine
• Thymus-expressed chemokine

Background

CCL25, also known as TECK (thymus-expressed chemokine), is a CC chemokine that regulates the trafficking of lymphocytes in the thymus and small intestine. Mature mouse CCL25 shares 40% and 81% amino acid sequence identity with human and rat CCL25, respectively (1). CCL25 is produced by stromal cells in the thymus and epithelial cells of the small intestine, particularly the jejunum and ileum (1-3). It binds to and induces chemoattraction through CCR9 (1, 4, 5), and both human and mouse proteins act on human CCR9 (4). CCR9 is expressed on immature pre-T cells and thymocytes (5, 6). CCL25 induces the homing of several lymphocyte populations to the small intestine (3), including Integrin alpha 4 beta 7⁺ gamma δ T cells (6, 7), Integrin alpha E beta 7⁺ CD8⁺ T cells (8), and IgA-producing plasma cells (2). In cancer, functional CCR9 mediates the metastasis of melanoma cells to the small intestine (9), contributes to the CCL25-dependent migration and invasion of some breast carcinomas (10), and attracts mesenchymal stromal cells to CCL25-expressing multiple myelomas (11). CCL25 contributes to the severity of chronic inflammation in rheumatoid arthritis where it attracts CCR9⁺ monocytes and macrophages (12), in endometriosis where it promotes the invasiveness of stromal cells (13), and in atherosclerosis where it contributes to the accumulation of CCR9⁺ macrophages in arterial plaques (14).

1. Vicari, A.P. et al. (1997) Immunity 7:291.
2. Bowman, E.P. et al. (2002) J. Exp. Med. 195:269.
3. Kunkel, E.J. et al. (2000) J. Exp. Med. 192:761.
4. Zaballos, A. et al. (1999) J. Immunol. 162:5671.
5. Youn, B.-S. et al. (1999) Blood 94:2533.
6. Uehara, S. et al. (2002) J. Immunol. 168:134.
7. Costa, M.F.S. et al. (2012) Eur. J. Immunol. 42:1250.
8. Svensson, M. et al. (2002) J. Clin. Invest. 110:1113.
9. Amersi, F.F. et al. (2008) Clin. Cancer Res. 14:638.
10. Johnson-Holiday, C. et al. (2011) Int. J. Oncol. 38:1279.
11. Xu, S. et al. (2012) Stem Cells 30:266.
12. Schmutz, C. et al. (2010) Arthritis Res. Ther. 12:R161.
13. Wang, Y. et al. (2010) Cell. Mol. Immunol. 7:51.
14. Alla, J.A. et al. (2010) J. Biol. Chem. 285:23496.

Publications for CCL25/TECK (481-TK/CF)(13)

Publications using 481-TK/CF

• SM Pontejo, C Sanchez, B Ruiz-Argüe, A Alcami Insights into ligand binding by a viral tumor necrosis factor (TNF) decoy receptor yield a selective soluble human type 2 TNF receptor J. Biol. Chem., 2019;0(0):. 2019 [PMID: 30723161] (Bioassay, Mouse)
• FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus Elife, 2018;7(0):. 2018 [PMID: 30187863] (Bioassay, Mouse)
• S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 2017;14(5):531-538. 2017 [PMID: 28394335] (Bioassay, Mouse)
• Chen H, Sun J, Huang Z, Hou H, Arcilla M, Rakhilin N, Joe D, Choi J, Gadamsetty P, Milsom J, Nandakumar G, Longman R, Zhou X, Edwards R, Chen J, Chen K, Bu P, Wang L, Xu Y, Munroe R, Abratte C, Miller A, Gumus Z, Shuler M, Nishimura N, Edelmann W, Shen X, Lipkin S Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting. Nat Biotechnol, 2015;33(6):656-60. 2015 [PMID: 26006007]
• Graham K, Zhang J, Lewen S, Burke T, Dang T, Zoudilova M, Sobel R, Butcher E, Zabel B A novel CMKLR1 small molecule antagonist suppresses CNS autoimmune inflammatory disease. PLoS ONE, 2014;9(12):e112925. 2014 [PMID: 25437209] (Bioassay, Mouse)
• Miething, Corneliu, Scuoppo, Claudio, Bosbach, Benedikt, Appelmann, Iris, Nakitandwe, Joy, Ma, Jing, Wu, Gang, Lintault, Laura, Auer, Martina, Premsrirut, Prem K, Teruya-Feldstein, Julie, Hicks, James, Benveniste, Helene, Speicher, Michael, Downing, James R, Lowe, Scott W PTEN action in leukaemia dictated by the tissue microenvironment. Nature, 2014;510(7505):402-6. 2014 [PMID: 24805236]
• Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction. Eur. J. Immunol., 2012;42(5):1250-60. 2012 [PMID: 22539297] (In Vivo, Mouse)
• McGuire HM, Vogelzang A, Ma CS, Hughes WE, Silveira PA, Tangye SG, Christ D, Fulcher D, Falcone M, King C A subset of interleukin-21+ chemokine receptor CCR9+ T helper cells target accessory organs of the digestive system in autoimmunity. Immunity, 2011;34(4):602-15. 2011 [PMID: 21511186] (Bioassay, Mouse)
• Penido C, Costa MF, Souza MC, Costa KA, Candea AL, Benjamim CF, Henriques MG Involvement of CC chemokines in gammadelta T lymphocyte trafficking during allergic inflammation: the role of CCL2/CCR2 pathway. Int. Immunol., 2007;20(1):129-39. 2007 [PMID: 18056919] (In Vivo, Mouse)
• Park EJ, Mora JR, Carman CV, Chen J, Sasaki Y, Cheng G, von Andrian UH, Shimaoka M Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut. J. Clin. Invest., 2007;117(9):2526-38. 2007 [PMID: 17786243] (Bioassay, Mouse)
• Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007;179(6):3724-33. 2007 [PMID: 17785809] (Bioassay, Mouse)
• Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2005;105(1):31-9. 2005 [PMID: 15358618] (Bioassay, Mouse)
• Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells. J. Immunol., 2004;173(6):3668-75. 2004 [PMID: 15356112] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ccl25
• Uniprot:
  • O35903.1()