Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.03‑0.1 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 3-15 ng/mL.
E. coli-derived mouse CCL19/MIP-3 beta protein Gly26-Val107-Leu-Glu, with a substitution of Ser108LeuGlu
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
9.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
small inducible cytokine subfamily A (Cys-Cys), member 19
Small-inducible cytokine A19
CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta chemokine that binds specifically to the chemokine receptor CCR‑7/EBI‑1/BLR‑2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of MIP-3 beta is down‑regulated by the anti‑inflammatory cytokine IL‑10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR-7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.
Kim, C.H. et al. (1998) J. Immunol. 160:2418.
Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
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