Recombinant Mouse CCL19/MIP-3 beta Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse CCL19/MIP-3 beta Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.03‑0.1 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived mouse CCL19/MIP-3 beta protein
Gly26-Val107-Leu-Glu, with a substitution of Ser108LeuGlu
Accession #
N-terminal Sequence
Gly26
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl19
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
9.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
440-M3/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL19/MIP-3 beta Protein, CF

  • beta chemokine exodus-3
  • Beta-chemokine exodus-3
  • CC chemokine ligand 19
  • C-C motif chemokine 19
  • CCL19
  • chemokine (C-C motif) ligand 19
  • CKb11
  • EBI1-ligand chemokine
  • ELC
  • ELCMIP-3-beta
  • Epstein-Barr virus-induced molecule 1 ligand chemokine
  • exodus-3
  • Macrophage inflammatory protein 3 beta
  • macrophage inflammatory protein 3-beta
  • MGC34433
  • MIP3 beta
  • MIP-3 beta
  • MIP-3b
  • MIP3BCK beta-11
  • SCYA19EBI1 ligand chemokine
  • small inducible cytokine subfamily A (Cys-Cys), member 19
  • Small-inducible cytokine A19

Background

CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR‑7/EBI‑1/BLR‑2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of MIP-3 beta is down‑regulated by the anti‑inflammatory cytokine IL‑10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR-7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

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Publications for CCL19/MIP-3 beta (440-M3/CF)(23)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(19)
In Vivo
(3)
All Applications
Filter By Species
Mouse
(22)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using 440-M3/CF Applications Species
M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista The Small Rho GTPase TC10 Modulates B Cell Immune Responses J. Immunol., 2017;0(0):. 2017 [PMID: 28747344] (Bioassay, Mouse) Bioassay Mouse
Z Dong, Y Chen, Y Peng, F Wang, Z Yang, G Huang, Y Chen, Z Yuan, T Cao, Y Peng Concurrent CCR7 Overexpression and RelB Knockdown in Immature Dendritic Cells Induces Immune Tolerance and Improves Skin-Graft Survival in a Murine Model Cell. Physiol. Biochem., 2017;42(2):455-468. 2017 [PMID: 28578354] (Bioassay, Mouse) Bioassay Mouse
H Xin, J Zhu, H Miao, Z Gong, X Jiang, X Feng, Y Tong Adenovirus-Mediated CCR7 and BTLA Overexpression Enhances Immune Tolerance and Migration in Immature Dendritic Cells Biomed Res Int, 2017;2017(0):3519745. 2017 [PMID: 28393074] (Bioassay, Mouse) Bioassay Mouse
IY Hwang, C Park, K Harrison, JH Kehrl Normal Thymocyte Egress, T Cell Trafficking, and CD4(+) T Cell Homeostasis Require Interactions between RGS Proteins and G?i2 J. Immunol, 2017;0(0):. 2017 [PMID: 28235863] (Bioassay, Mouse) Bioassay Mouse
Tianliang Sun A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib J. Cell Biol, 2016;0(0):. 2016 [PMID: 28007914] (Bioassay, Mouse) Bioassay Mouse
Istvan Szatmari Immunogenic Dendritic Cell Generation from Pluripotent Stem Cells by Ectopic Expression of Runx3 J. Immunol., 2016;0(0):. 2016 [PMID: 27852743] (Bioassay, Mouse) Bioassay Mouse
A microfluidic device for measuring cell migration towards substrate-bound and soluble chemokine gradients Sci Rep, 2016;6(0):36440. 2016 [PMID: 27819270] (Bioassay, Mouse) Bioassay Mouse
CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima Nat Immunol, 2016;0(0):. 2016 [PMID: 27668800] (In Vivo, Mouse) In Vivo Mouse
Tsun-Ho Lo Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration J Immunol, 2016;0(0):. 2016 [PMID: 27316686] (Bioassay, Mouse) Bioassay Mouse
Poon G, Dong Y, Marshall K, Arif A, Deeg C, Dosanjh M, Johnson P Hyaluronan Binding Identifies a Functionally Distinct Alveolar Macrophage-like Population in Bone Marrow-Derived Dendritic Cell Cultures. J Immunol, 2015;195(2):632-42. 2015 [PMID: 26085682] (Bioassay, Mouse) Bioassay Mouse
Show All 23 Publications.

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Bioinformatics

Gene Symbol Ccl19
Entrez
Uniprot