Recombinant Mouse BMP-4 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse BMP-4 Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 2-12 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse BMP-4 protein
Ser293-Arg408
Accession #
N-terminal Sequence
Ser293
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Bmp4
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-26 kDa, reducing conditions
Publications
Read Publications using
5020-BP/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BMP-4 Protein, CF

  • BMP-2B
  • BMP2B1
  • BMP2BMCOPS6
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • OFC11
  • ZYME

Background

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The mouse BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). The propeptide is cleaved intracellularly by furin or proprotein convertase 6, enabling the 15 kDa mature BMP-4 monomer to form an active disulfide linked homodimer or heterodimer with BMP-7 (7-9). Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85% aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).

  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Oida, S. et al. (1995) DNA Seq. 5:273.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

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5020-BP/CF
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Publications for BMP-4 (5020-BP/CF)(64)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Rabbit, Sea Urchin.

We have publications tested in 4 applications: Bioassay, Cell Culture Substrate, ELISA capture, In Vivo.


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Bioassay
(60)
Cell Culture Substrate
(1)
ELISA capture
(1)
In Vivo
(1)
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Human
(1)
Mouse
(60)
Rat
(1)
Rabbit
(1)
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Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using 5020-BP/CF Applications Species
P Andersen, E Tampakakis, DV Jimenez, S Kannan, M Miyamoto, HK Shin, A Saberi, S Murphy, E Sulistio, SP Chelko, C Kwon Precardiac organoids form two heart fields via Bmp/Wnt signaling Nat Commun, 2018;9(1):3140. 2018 [PMID: 30087351] (Mouse) Mouse
T Bongiorno, J Gura, P Talwar, D Chambers, KM Young, D Arafat, G Wang, EL Jackson-Ho, P Qiu, TC McDevitt, T Sulchek Biophysical subsets of embryonic stem cells display distinct phenotypic and morphological signatures PLoS ONE, 2018;13(3):e0192631. 2018 [PMID: 29518080] (Bioassay, Mouse) Bioassay Mouse
Y Chen, H Xu, G Lin Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse Cell Discov, 2017;3(0):17046. 2017 [PMID: 29263795] (Bioassay, Mouse) Bioassay Mouse
C Yoon, H Song, T Yin, D Bausch-Flu, AP Frei, S Kattman, N Dubois, AD Witty, JA Hewel, H Guo, A Emili, B Wollscheid, G Keller, PW Zandstra FZD4 Marks Lateral Plate Mesoderm and Signals with NORRIN to Increase Cardiomyocyte Induction from Pluripotent Stem Cell-Derived Cardiac Progenitors Stem Cell Reports, 2017;0(0):. 2017 [PMID: 29249665] (Bioassay, Mouse) Bioassay Mouse
S Bao, WW Tang, B Wu, S Kim, J Li, L Li, T Kobayashi, C Lee, Y Chen, M Wei, S Li, S Dietmann, F Tang, X Li, MA Surani Derivation of hypermethylated pluripotent embryonic stem cells with high potency Cell Res., 2017;0(0):. 2017 [PMID: 29076502] (Bioassay, Mouse) Bioassay Mouse
SN Kim, HJ Kwon, SW Im, YH Son, S Akindehin, YS Jung, SJ Lee, IJ Rhyu, IY Kim, JK Seong, J Lee, HC Yoo, JG Granneman, YH Lee Connexin 43 is required for the maintenance of mitochondrial integrity in brown adipose tissue Sci Rep, 2017;7(1):7159. 2017 [PMID: 28769076] (Bioassay, Mouse) Bioassay Mouse
JD Glover, KL Wells, F Matthäus, KJ Painter, W Ho, J Riddell, JA Johansson, MJ Ford, CAB Jahoda, V Klika, RL Mort, DJ Headon Hierarchical patterning modes orchestrate hair follicle morphogenesis PLoS Biol., 2017;15(7):e2002117. 2017 [PMID: 28700594] (Bioassay, Mouse) Bioassay Mouse
J Feng, J Jing, J Li, H Zhao, V Punj, T Zhang, J Xu, Y Chai BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice Development, 2017;0(0):. 2017 [PMID: 28576771] (Bioassay, Mouse) Bioassay Mouse
Y Liu, SY Du, M Ding, X Dou, FF Zhang, ZY Wu, SW Qian, W Zhang, QQ Tang, CJ Xu The BMP4-Smad Signaling Pathway Regulates Hyperandrogenism Development in a Female Mouse Model J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28572510] (Bioassay, Mouse) Bioassay Mouse
H Ohta, K Kurimoto, I Okamoto, T Nakamura, Y Yabuta, H Miyauchi, T Yamamoto, Y Okuno, M Hagiwara, K Shirane, H Sasaki, M Saitou In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate EMBO J., 2017;0(0):. 2017 [PMID: 28559416] (Bioassay, Mouse) Bioassay Mouse
Show All 64 Publications.

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Bioinformatics

Gene Symbol Bmp4
Uniprot