Recombinant Mouse beta-NGF Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse beta-NGF Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.1-1 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse beta-NGF protein
  Ser122-Gly241
• Accession #: Q6LDU8
• N-terminal Sequence: Ser122
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ngf
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse beta-NGF Protein

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2).  beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and gamma . It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF (6).

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).

1. Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
2. Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
3. Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
4. Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
5. Angeletti, R H. et al. (1973) Biochemistry 12:100.
6. Ullrich, A. et al. (1983) Nature 303:821.
7. Gotz, R. et al. (1994) Nature 372:266.
8. Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
9. Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
10. E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.

Publications for beta-NGF (1156-NG)(20)

Publications using 1156-NG

• Hampton, RF;Jimenez-Gonzalez, M;Kang, R;Alvarsson, A;Espinoza, D;Carty, JRE;Seibold, F;Lambertini, L;Alexeyev, L;Li, R;Devarakonda, K;Lu, G;Agyapong, B;Choudhury, J;Alon, M;Scott, D;Stewart, A;Garcia-Ocana, A;Stanley, SA; Nutrient excess remodels islet autonomic innervation via pancreatic schwann cells bioRxiv : the preprint server for biology 2025-07-05 [PMID: 40631185] (Bioassay, Mouse)
• Deng, T;Wu, X;Wang, Y;Fan, X;Hu, B; Toe1 promotes proliferation and differentiation of neural progenitor cells Heliyon 2024-10-30 [PMID: 39502232] (Bioassay, Mouse)
• El Chemali, L;Boutary, S;Liu, S;Liu, GJ;Middleton, RJ;Banati, RB;Bahrenberg, G;Rupprecht, R;Schumacher, M;Massaad-Massade, L; GRT-X Stimulates Dorsal Root Ganglia Axonal Growth in Culture via TSPO and Kv7.2/3 Potassium Channel Activation International journal of molecular sciences 2024-07-03 [PMID: 39000434] (Bioassay, Mouse, Transgenic Mouse)
• J Sarkar, Y Luo, Q Zhou, E Ivakhnitsk, D Lara, E Katz, MG Sun, V Guaiquil, M Rosenblatt VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types PLoS ONE, 2022-07-21;17(7):e0269818. 2022-07-21 [PMID: 35862373] (Bioassay, Rat)
• P Pagella, J Catón, CT Meisel, TA Mitsiadis Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons Cells, 2020-03-06;9(3):. 2020-03-06 [PMID: 32155948] (Cell Culture, Mouse)
• Y Gu, W Wei, Y Cheng, B Wan, X Ding, H Wang, Y Zhang, M Jin A pivotal role of BEX1 in liver progenitor cell expansion in mice Stem Cell Res Ther, 2018-06-15;9(1):164. 2018-06-15 [PMID: 29907129] (Bioassay, Mouse)
• Y Zhai, G Yao, F Rao, Y Wang, X Song, F Sun Excessive nerve growth factor impairs bidirectional communication between the oocyte and cumulus cells resulting in reduced oocyte competence Reprod. Biol. Endocrinol., 2018-03-27;16(1):28. 2018-03-27 [PMID: 29580253] (Bioassay, Mouse)
• LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko Epiregulin and EGFR interactions are involved in pain processing J. Clin. Invest., 2017-08-07;0(0):. 2017-08-07 [PMID: 28783046] (In Vivo, Mouse)
• F Zeng, Z Li, Q Zhu, R Dong, C Zhao, G Li, G Li, W Gao, G Jiang, E Zheng, G Cai, S Moisyadi, J Urschitz, H Yang, D Liu, Z Wu Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors Sci Rep, 2017-01-24;7(0):41270. 2017-01-24 [PMID: 28117418] (Bioassay, Western Blot, Human)
• Laguerre Filter Analysis with Partial Least Square Regression Reveals a Priming Effect of ERK and CREB on c-FOS Induction PLoS ONE, 2016-08-11;11(8):e0160548. 2016-08-11 [PMID: 27513954] (Bioassay, Rat)
• Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy. PLoS ONE, 2014-02-26;9(2):e89881. 2014-02-26 [PMID: 24587096] (Bioassay, Mouse)
• Hedstrom K, Murtie J, Albers K, Calcutt N, Corfas G Treating small fiber neuropathy by topical application of a small molecule modulator of ligand-induced GFRalpha/RET receptor signaling. Proc Natl Acad Sci U S A, 2014-01-21;111(6):2325-30. 2014-01-21 [PMID: 24449858] (Bioassay, Rat)
• Wang Y, Liang N, Yao G, Tian H, Zhai Y, Yin Y, Sun F Knockdown of TrkA in cumulus oocyte complexes (COCs) inhibits EGF-induced cumulus expansion by down-regulation of IL-6. Mol Cell Endocrinol, 2013-11-08;382(2):804-13. 2013-11-08 [PMID: 24215827] (Bioassay, Mouse)
• Vaishnavi A, Capelletti M, Le A, Kako S, Butaney M, Ercan D, Mahale S, Davies K, Aisner D, Pilling A, Berge E, Kim J, Sasaki H, Park S, Kryukov G, Garraway L, Hammerman P, Haas J, Andrews S, Lipson D, Stephens P, Miller V, Varella-Garcia M, Janne P, Doebele R Oncogenic and drug-sensitive NTRK1 rearrangements in lung cancer. Nat Med, 2013-10-27;19(11):1469-72. 2013-10-27 [PMID: 24162815] (Bioassay, Mouse)
• Howard , Laura, Wyatt , Sean, Nagappan , Guhan, Davies , Alun M ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75NTR-dependent mechanism. Development, 2013-05-01;140(10):2108-17. 2013-05-01 [PMID: 23633509] (Bioassay, Mouse)
• Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013-03-20;33(12):5399-410. 2013-03-20 [PMID: 23516305] (Bioassay, Mouse)
• Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6. 2012-06-04 [PMID: 22665806] (Bioassay, Mouse)
• Ralainirina N, Brons NH, Ammerlaan W, Hoffmann C, Hentges F, Zimmer J Mouse natural killer (NK) cells express the nerve growth factor receptor TrkA, which is dynamically regulated. PLoS ONE, 2010-12-01;5(12):e15053. 2010-12-01 [PMID: 21152021] (Bioassay, Mouse)
• Satoh A, Cummings GM, Bryant SV, Gardiner DM Neurotrophic regulation of fibroblast dedifferentiation during limb skeletal regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol., 2009-11-24;337(2):444-57. 2009-11-24 [PMID: 19944088] (In Vivo, Amphibian)
• Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation. Development, 2007-09-01;134(18):3327-38. 2007-09-01 [PMID: 17720696] (Bioassay, Mouse, Rat)

Bioinformatics

• Gene Symbol: Ngf
• Uniprot:
  • Q6LDU8()