Recombinant Mouse beta-NGF Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse beta-NGF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.1-1 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse beta-NGF protein
Ser122-Gly241
Accession #
N-terminal Sequence
Ser122
Protein/Peptide Type
Recombinant Proteins
Gene
Ngf
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1156-NG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse beta-NGF Protein

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428

Background

NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2).  beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF (6).

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).

  1. Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
  2. Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
  3. Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
  4. Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
  5. Angeletti, R H. et al. (1973) Biochemistry 12:100.
  6. Ullrich, A. et al. (1983) Nature 303:821.
  7. Gotz, R. et al. (1994) Nature 372:266.
  8. Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
  9. Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
  10. E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.

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1156-NG
Species: Mu
Applications: Bioactivity

Publications for beta-NGF (1156-NG)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Amphibian.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(11)
In Vivo
(2)
All Applications
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Human
(1)
Mouse
(9)
Rat
(2)
Amphibian
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 1156-NG Applications Species
Y Gu, W Wei, Y Cheng, B Wan, X Ding, H Wang, Y Zhang, M Jin A pivotal role of BEX1 in liver progenitor cell expansion in mice Stem Cell Res Ther, 2018;9(1):164. 2018 [PMID: 29907129] (Bioassay, Mouse) Bioassay Mouse
Y Zhai, G Yao, F Rao, Y Wang, X Song, F Sun Excessive nerve growth factor impairs bidirectional communication between the oocyte and cumulus cells resulting in reduced oocyte competence Reprod. Biol. Endocrinol., 2018;16(1):28. 2018 [PMID: 29580253] (Bioassay, Mouse) Bioassay Mouse
LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko Epiregulin and EGFR interactions are involved in pain processing J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28783046] (In Vivo, Mouse) In Vivo Mouse
F Zeng, Z Li, Q Zhu, R Dong, C Zhao, G Li, G Li, W Gao, G Jiang, E Zheng, G Cai, S Moisyadi, J Urschitz, H Yang, D Liu, Z Wu Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors Sci Rep, 2017;7(0):41270. 2017 [PMID: 28117418] (Bioassay, Human) Bioassay Human
Laguerre Filter Analysis with Partial Least Square Regression Reveals a Priming Effect of ERK and CREB on c-FOS Induction PLoS ONE, 2016;11(8):e0160548. 2016 [PMID: 27513954] (Bioassay, Rat) Bioassay Rat
Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy. PLoS ONE, 2014;9(2):e89881. 2014 [PMID: 24587096] (Bioassay, Mouse) Bioassay Mouse
Hedstrom K, Murtie J, Albers K, Calcutt N, Corfas G Treating small fiber neuropathy by topical application of a small molecule modulator of ligand-induced GFRalpha/RET receptor signaling. Proc Natl Acad Sci U S A, 2014;111(6):2325-30. 2014 [PMID: 24449858] (Bioassay, Rat) Bioassay Rat
Wang Y, Liang N, Yao G, Tian H, Zhai Y, Yin Y, Sun F Knockdown of TrkA in cumulus oocyte complexes (COCs) inhibits EGF-induced cumulus expansion by down-regulation of IL-6. Mol Cell Endocrinol, 2014;382(2):804-13. 2014 [PMID: 24215827] (Bioassay, Mouse) Bioassay Mouse
Howard, Laura, Wyatt, Sean, Nagappan, Guhan, Davies, Alun M ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75NTR-dependent mechanism. Development, 2013;140(10):2108-17. 2013 [PMID: 23633509] (Bioassay, Mouse) Bioassay Mouse
Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013;33(12):5399-410. 2013 [PMID: 23516305] (Bioassay, Mouse) Bioassay Mouse
Show All 14 Publications.

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Bioinformatics

Gene Symbol Ngf
Entrez
Uniprot