Recombinant Mouse B7-H2 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse B7-H2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured in a competitive binding assay. When rmICOS/Fc Chimera (Catalog # 168-CS) is immobilized at 1 µg/mL (100 µL/well), rmB7-H2 inhibits 50% binding of biotinylated rhB7-H2/Fc Chimera (2.5 ng/mL) at the concentration range of 80‑120 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse B7-H2 protein
  

  Mouse B7-H2 (Glu47 - Lys279) Accession # Q9JHJ8	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q9JHJ8
• N-terminal Sequence: Glu47
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Icosl
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 53 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse B7-H2 Fc Chimera Protein, CF

• B7H2
• B7-H2
• B7H2B7 homolog 2
• B7-H2B7-related protein 1
• B7RP-1
• B7RP1B7-like protein Gl50
• B7RP-1LICOS
• CD275 antigen
• CD275
• GL50
• GL50transmembrane protein B7-H2 ICOS ligand
• ICOSL
• ICOS-L
• ICOSLG
• inducible T-cell co-stimulator ligand
• KIAA0653ICOS ligand

Background

Mouse B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune co‑stimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. The extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Mouse and human B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of co‑stimulatory receptors. Mouse B7-H2 is a 322 amino acid (aa) protein with a putative 46 aa signal peptide, a 233 aa extracellular domain, a 19 aa transmembrane region, and a 24 aa cytoplasmic domain. Mouse B7-H2 is expressed constitutively on resting B cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
2. Ling, V. et al. (2000) J. Immunol. 164:1653.
3. Wang, S. et al. (2000) Blood 96:2808.
4. Brodie, D. et al. (2000) Curr. Biol. 10:333.
5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
6. Swallow, M.M. et al. (1999) Immunity 11:423.
7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Publications for B7-H2/ICOSLG (158-B7)(1)

Publication using 158-B7

• M Montes-Cas, G Ojeda, L Aragoneses, D López, B de Andrés, ML Gaspar, U Dianzani, JM Rojo, P Portolés ICOS deficiency hampers the homeostasis, development and function of NK cells PLoS ONE, 2019-07-08;14(7):e0219449. 2019-07-08 [PMID: 31283790] (Cell Culture, Mouse)

Bioinformatics

• Gene Symbol: Icosl
• Uniprot:
  • Q9JHJ8()