Recombinant Mouse B7-1/CD80 His tagged Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse B7-1/CD80 His tagged Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse CTLA-4 Fc Chimera (Catalog # 434-CT) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse B7-1/CD80 that produces 50% of the optimal binding response is approximately 5-30 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse B7-1/CD80 protein
  Asp37-Lys245, with a C-terminal 6-His tag
• Accession #: Q00609
• N-terminal Sequence: Asp37
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd80
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 39-54 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse B7-1/CD80 His tagged Protein, CF

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature mouse B7-1 consists of a 209 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane segment, and a 38 aa cytoplasmic domain (7). Within the ECD, mouse B7-1 shares 50% and 68% aa sequence identity with human and rat B7-1, respectively. Alternative splicing generates a short isoform that lacks the second Ig-like domain but retains the ability to bind CD28 (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2). B7-1 and B7-2 interact with CD28 to modulate short- and long-lived plasma cell functions.

1. Ville, S. et al. (2015) Front. Immunol. 6:411.
2. Azuma, M. et al. (1993) Nature 366:76.
3. Freeman, G.J. et al. (1993) Science 262:909.
4. Chen, C. et al. (1994) J. Immunol. 152:4929.
5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
6. Lanier, L. et al. (1995) J. Immunol. 154:97.
7. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
8. Inobe, M. et al. (1994) Biochem. Biophys. Res. Commun. 200:443.
9. Njan, MN. et al. (2012) J. Immunol. 189:2758

Bioinformatics

• Gene Symbol: Cd80
• Uniprot:
  • Q00609()