Recombinant Mouse Angiopoietin-like 3 Protein, CF

Images

 
There are currently no images for Angiopoietin-like Protein 3/ANGPTL3 (136-AN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Inhibition Activity
Format
Carrier-Free

Order Details

Recombinant Mouse Angiopoietin-like 3 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770. The IC50 value under conditions in which lipoprotein lipase is present at 5 units/mL, and p-nitrophenyl butyrate concentration is 1 mM in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is approximately 2.5‑12.5 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Angiopoietin-like Protein 3/ANGPTL3 protein
Ser17-Thr455, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ser17
Structure / Form
Oligomer
Protein/Peptide Type
Recombinant Proteins
Gene
Angptl3
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
51.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa, reducing conditions
Publications
Read Publications using
136-AN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

Triton is a registered trademark of Union Carbide Corp.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Angiopoietin-like 3 Protein, CF

  • AGNPT5
  • Ang-5
  • angiopoietin 5
  • Angiopoietin-5
  • angiopoietin-like 3
  • Angiopoietin-like Protein 3
  • angiopoietin-related protein 3
  • ANGPT5angiopoietin-5
  • ANGPTL3
  • FHBL2

Background

Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins (1 - 3). Mature mouse ANGPTL3 contains an N-terminal coiled-coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C‑terminal fragments containing the coiled‑coil domain and fibrinogen-like domains, respectively (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL), an enzyme responsible for hydrolyzing circulating triglycerides (8). This activity requires a putative heparin-binding motif that is N-terminal to the coiled-coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6 - 8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (9). ANGPTL3 expression in vivo is upregulated by LXR agonists and downregulated by insulin, leptin, and TR beta agonists (10 - 12). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice, (7, 8, 11). ANGPTL3 does not bind Tie-1 or Tie-2 but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (13). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (14). Mature mouse ANGPTL3 shares 22% - 30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 6, and 7. It shares 77% aa sequence identity with human ANGPTL3.

  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
  3. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
  4. Conklin, D. et al. (1999) Genomics 62:477.
  5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  7. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Koster, A. et al. (2005) Endocrinology 146:4943.
  10. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  11. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  12. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  13. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  14. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Customers Who Viewed This Item Also Viewed...

AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
AF3485
Species: Hu, Pm
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP1-88057
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NB400-111
Species: Hu, Mu(-)
Applications: WB, ICC/IF, IHC-Fr
NBP2-15394
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB200-527
Species: Hu, Mu
Applications: WB
NBP2-46205
Species: Hu
Applications: WB, IHC
AF1444
Species: Mu
Applications: WB, IHC
NBP1-83394
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-41537
Species: Hu, Mu, Rt, Bv
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NBP1-85870
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB400-139
Species: Hu, Ha
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for Angiopoietin-like Protein 3/ANGPTL3 (136-AN)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for Angiopoietin-like Protein 3/ANGPTL3 (136-AN) (0)

There are no reviews for Angiopoietin-like Protein 3/ANGPTL3 (136-AN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Angiopoietin-like Protein 3/ANGPTL3 (136-AN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Angiopoietin-like Protein 3/ANGPTL3 (136-AN)

Discover related pathways, diseases and genes to Angiopoietin-like Protein 3/ANGPTL3 (136-AN). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Angiopoietin-like Protein 3/ANGPTL3 (136-AN)

Discover more about diseases related to Angiopoietin-like Protein 3/ANGPTL3 (136-AN).
 

Pathways for Angiopoietin-like Protein 3/ANGPTL3 (136-AN)

View related products by pathway.

PTMs for Angiopoietin-like Protein 3/ANGPTL3 (136-AN)

Learn more about PTMs related to Angiopoietin-like Protein 3/ANGPTL3 (136-AN).

Blogs on Angiopoietin-like Protein 3/ANGPTL3

There are no specific blogs for Angiopoietin-like Protein 3/ANGPTL3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Angiopoietin-like 3 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Angptl3
Entrez
Uniprot