Recombinant Mouse Angiopoietin-like 3 (aa 17-220) Biotin, CF

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Both Biotinylated rmANGPTL3 (Catalog # BT8344) and unlabeled rmANGPTL3 (Catalog # 8344-AN) stimulate the expansion of E16-E18 rat liver mononuclear cells link, in the presence of rmSCF (Catalog # 455-MC), ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Angiopoietin-like 3 (aa 17-220) Biotin, CF Summary

Additional Information
N-Terminal Fragment
Details of Functionality
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is 20-120 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Angiopoietin-like Protein 3/ANGPTL3 protein
Ser17-Thr206, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser17
Structure / Form
Biotinylated protein via sugars
Protein/Peptide Type
Recombinant Proteins
Gene
Angptl3
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
23 kDa (unlabeled).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Angiopoietin-like 3 (aa 17-220) Biotin, CF

  • AGNPT5
  • Ang-5
  • angiopoietin 5
  • Angiopoietin-5
  • angiopoietin-like 3
  • Angiopoietin-like Protein 3
  • angiopoietin-related protein 3
  • ANGPT5angiopoietin-5
  • ANGPTL3
  • FHBL2

Background

Angiopoietin-like Protein 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins (1). Mature mouse ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (2). Mature mouse ANGPTL3 shares 77% aa sequence identity with human ANGPTL3. ANGPTL3 is expressed in the liver from early in development through adulthood (2, 3). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated
N- and C-terminal fragments containing the coiled coil domain and fibrinogen-like domains, respectively (4, 5). The cleavage of ANGPTL3 by Furin and Proprotein Convertase 5/6 is enhanced by its interaction with the related ANGPTL8 (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (3, 4). ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled coil domain (4). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo [Ono 41804]. ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (4, 5, 8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is upregulated by LXR agonists and downregulated by insulin, leptin, and agonists of TR beta or PPAR beta (11-14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (5, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with Integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (16).
  1. Santulli, G. (2014) Front. Endocrinol. (Lausanne) 5:4.
  2. Conklin, D. et al. (1999) Genomics 62:477.
  3. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  4. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  5. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  6. Essalmani, R. et al. (2013) J. Biol. Chem. 288:26410.
  7. Quagliarini, F. et al. (2012) Proc. Natl. Acad. Sci. USA 109:19751.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366.
  10. Koster, A. et al. (2005) Endocrinology 146:4943.
  11. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  12. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  13. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  14. Matsusue, K. et al. (2006) Mol. Cell Endocrinol. 256:23.
  15. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  16. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

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Bioinformatics

Gene Symbol Angptl3
Entrez
Uniprot