Recombinant Mouse ALCAM Fc Chimera Protein, CF

Images

 
There are currently no images for ALCAM/CD166 (1172-AL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse ALCAM Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to block adhesion of HuT 78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6 Fc Chimera (Catalog # 627-CD). Recombinant Mouse ALCAM/CD166 Fc Chimera at 10 µg/mL (50 µL/well) blocked >90% of HUT-78 cell adhesion (1 x 106 cells/mL, 50 µL/well) to immobilized recombinant human CD6 Fc Chimera (10 µg/mL, 100 µL/well).
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse ALCAM/CD166 protein
Mouse ALCAM
(Trp28-Lys527)
Accession # AAC06342
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Trp28
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Alcam
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
82 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
114-122 kDa, reducing conditions
Publications
Read Publications using
1172-AL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ALCAM Fc Chimera Protein, CF

  • activated leucocyte cell adhesion molecule
  • activated leukocyte cell adhesion moleculeFLJ38514
  • ALCAM
  • CD166 antigen
  • CD166
  • MEMDMGC71733

Background

ALCAM (activated leukocyte cell adhesion molecule), designated CD166 and also called MEMD and SC‑1/DM‑GRASP/BEN in the chicken, is a 100 ‑ 110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte‑derived dendritic cells (1, 2). Mouse ALCAM cDNA encodes 583 amino acid (aa), including signal peptide (27 aa), extracellular domain (ECD, 500 aa) with two V‑type and three C2‑type Ig‑like domains, transmembrane (22  aa) and cytoplasmic (34 aa) domains (1). Mouse ALCAM ECD shares 98%, 93% and 92% aa sequence identity with rat, human/porcine and bovine/equine ALCAM, respectively. A secreted isoform in endothelial cells that is truncated at aa 133 (sALCAM) antagonizes full‑length ALCAM (3, 4). ALCAM mediates low‑affinity adhesion with itself or the cysteine‑rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1 ‑ 11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM‑expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (2, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on human melanoma cell lines appears to be pro‑metastatic, but anti‑metastatic activity has been reported in human breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM‑L1, both in the developing retina and invasive melanoma (3, 12).

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Zimmerman, A.W. et al. (2006) Blood 107:3212.
  3. van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
  4. Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
  5. van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
  6. Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
  7. Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
  8. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
  9. Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
  10. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  11. King, J.A. et al. (2010) Mol. Cancer 9:266.
  12. Buhusi, M. et al. (2009) J. Neurosci. 29:15630.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-36561
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, B/N
NB100-77417
Species: Hu, Mu, Rt
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, In vivo, CyTOF-ready
NB100-65543
Species: Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IP
NBP1-85740
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-56244
Species: Hu
Applications: WB, ICC/IF
AF3628
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NB120-16518
Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-41083
Species: Hu
Applications: WB, ChIP, ELISA, ICC/IF
NBP2-93743
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
AF932
Species: Hu
Applications: WB, Simple Western, IHC, ICC

Publications for ALCAM/CD166 (1172-AL)(3)

Reviews for ALCAM/CD166 (1172-AL) (0)

There are no reviews for ALCAM/CD166 (1172-AL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for ALCAM/CD166 (1172-AL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional ALCAM/CD166 Products

Bioinformatics Tool for ALCAM/CD166 (1172-AL)

Discover related pathways, diseases and genes to ALCAM/CD166 (1172-AL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ALCAM/CD166 (1172-AL)

Discover more about diseases related to ALCAM/CD166 (1172-AL).
 

Pathways for ALCAM/CD166 (1172-AL)

View related products by pathway.

PTMs for ALCAM/CD166 (1172-AL)

Learn more about PTMs related to ALCAM/CD166 (1172-AL).

Blogs on ALCAM/CD166

There are no specific blogs for ALCAM/CD166, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse ALCAM Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Alcam
Uniprot