Recombinant Human WIF-1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human WIF-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.3-1.5 µg/mL in the presence of 10 ng/mL of Wnt-3a.
Source
Mouse myeloma cell line, NS0-derived human WIF-1 protein
Gly29-Trp379, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Gly29
Protein/Peptide Type
Recombinant Proteins
Gene
WIF1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
39.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50 kDa, reducing conditions
Publications
Read Publications using
1341-WF/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetic Acid, NaCl, PEG3350 and CHAPS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human WIF-1 Protein, CF

  • WIF1
  • WIF-1
  • WNT inhibitory factor 1

Background

WIF (Wnt Inhibitory Factor) is a secreted protein that binds to Wnt proteins and inhibits their activity. The protein is synthesized as a 379 amino acid (AA) molecule that contains an N-terminal signal sequence, 150 AA WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus (1). In situ hybridization analysis from the frog, Xenopus laevis, and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles (1). WIF inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos (1). In vitro, WIF binds to Drosophila Wingless and Xenopus Wnt8 proteins (1). WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung and bladder (2). However, WIF-1’s role in carcinogenesis may not be that simple since in other cancer types, such as colon adenocarcinoma, WIF facilitates tumorigenesis (3). Human WIF-1 shares 94% and 82% amino acid identity with mouse and frog, respectively.

  1. Hsieh, J-C. et al. (1999) Nature 398:431.
  2. Wissmann, C. et al. (2003) J. Pathol 201:204.
  3. Cebrat, M. et al. (2004) Cancer Lett. 206:107.

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Publications for WIF-1 (1341-WF/CF)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(10)
All Applications
Filter By Species
Human
(7)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 10.
Publications using 1341-WF/CF Applications Species
S Pan, Y Deng, J Fu, Y Zhang, Z Zhang, X Ru, X Qin Tumor Suppressive Role of ARHGAP17 in Colon Cancer Through Wnt/?-Catenin Signaling Cell. Physiol. Biochem., 2018;46(5):2138-2148. 2018 [PMID: 29730655] (Bioassay, Human) Bioassay Human
T Skaria, E Bachli, G Schoedon WIF1 prevents Wnt5A mediated LIMK/CFL phosphorylation and adherens junction disruption in human vascular endothelial cells J Inflamm (Lond), 2017;14(0):10. 2017 [PMID: 28529460] (Bioassay, Human) Bioassay Human
Scalable Differentiation of Human iPSCs in a Multicellular Spheroid-based 3D Culture into Hepatocyte-like Cells through Direct Wnt/?-catenin Pathway Inhibition Sci Rep, 2016;6(0):32888. 2016 [PMID: 27616299] (Bioassay, Human) Bioassay Human
IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling PLoS ONE, 2016;11(5):e0156002. 2016 [PMID: 27214384] (Bioassay, Human) Bioassay Human
T Skaria, E Bachli, G Schoedon Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells Cell Adh Migr, 2017;11(1):24-38. 2017 [PMID: 27159116] (Bioassay, Human) Bioassay Human
Tsakiridis, Anestis, Huang, Yali, Blin, Guillaum, Skylaki, Stavroul, Wymeersch, Filip, Osorno, Rodrigo, Economou, Costas, Karagianni, Eleni, Zhao, Suling, Lowell, Sally, Wilson, Valerie Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors. Development, 2014;141(6):1209-21. 2014 [PMID: 24595287] (Bioassay, Mouse) Bioassay Mouse
Keil K, Mehta V, Branam A, Abler L, Buresh-Stiemke R, Joshi P, Schmitz C, Marker P, Vezina C Wnt inhibitory factor 1 (Wif1) is regulated by androgens and enhances androgen-dependent prostate development. Endocrinology, 2012;153(12):6091-103. 2012 [PMID: 23087175] (Bioassay, Mouse) Bioassay Mouse
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Gudjonsson JE, Johnston A, Stoll SW Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 2010;130(7):1849-59. 2010 [PMID: 20376066] (Bioassay, Human) Bioassay Human
Cho SW, Yang JY, Sun HJ, Jung JY, Her SJ, Cho HY, Choi HJ, Kim SW, Kim SY, Shin CS Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells. Bone, 2009;44(6):1069-77. 2009 [PMID: 19254785] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol WIF1
Entrez
Uniprot