WIF-1 Antibody (133015) [Unconjugated]

WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 133015
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

WIF-1 Antibody (133015) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human WIF-1
  Gly29-Trp379
  Accession # AAD25402
• Specificity: Detects human WIF-1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse WIF‑1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: WIF1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WIF-1 Antibody (133015) [Unconjugated]

• WIF1
• WIF-1
• WNT inhibitory factor 1

Background

WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WIF-1 Antibody (MAB134)(3)

Publications using MAB134

• Gudjonsson JE, Johnston A, Stoll SW Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 2010;130(7):1849-59. 2010 [PMID: 20376066] (IHC-Fr, Human)
• Kawakami K, Hirata H, Yamamura S, Kikuno N, Saini S, Majid S, Tanaka Y, Kawamoto K, Enokida H, Nakagawa M, Dahiya R Functional significance of Wnt inhibitory factor-1 gene in kidney cancer. Cancer Res., 2009;69(22):8603-10. 2009 [PMID: 19887605] (IHC-P, Human)
• Elston MS, Gill AJ, Conaglen JV, Clarkson A, Shaw JM, Law AJ, Cook RJ, Little NS, Clifton-Bligh RJ, Robinson BG, McDonald KL Wnt pathway inhibitors are strongly down-regulated in pituitary tumors. Endocrinology, 2007;149(3):1235-42. 2007 [PMID: 18079202] (IHC-P, Human)

Review for WIF-1 Antibody (MAB134) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 02/19/2019

Summary

• Application: Western Blot
• Sample Tested: HCT116 cell line lysate
• Species: Human
• Lot: EZP0416081

Comments

• Comments: Human HCT116 cells transfected with WIF1 expression vector for 24 hours.

Bioinformatics

• Gene Symbol: WIF1
• Uniprot:
  • AAD25402()